Team:Aix-Marseille/Composite Part
Antifreeze proteins (BBa_K2915150)
The AFPs or Ice Structuring Proteins (ISPs) is a class of polypeptide produced by certain animals, plants and bacteria. they promote their survival in subzero environment. Why we've used Antifreeze ? We've used them to avoid the destruction of our designed proteins and to limite the formation of crystal water into the sample. So, these proteins have a protection role in our system. We've used IDT tool to optimize protein production in E.coli. We used BBa_K525998 as a T7-RBS. These elements are inducible by IPTG. This part uses the RFC 10/25 standard.
TAL1 (BBa_K2915225)
The TAL1 gene is a binding protein for the amplicon. We've used the protein sequence to optimize its synthesis by E.coli. The part uses RFC 10 standards. We've used BBa_K525998 as a RBS and T7 promoter that are inducible by IPTG. We have also tested production of the protein and used the His-tag to purify the protein.
TAL2 (BBa_K2915275)
The TAL2 gene is a binding protein for the amplicon. We've used the protein sequence to optimize its synthesis in E.coli. The part RFC 10 standards were used. We've used BBa_K525998 as a RBS and T7 promoter that are inducible by IPTG. We have also tested the production of the protein and used a His-tag to purify the protein. We've tested the activity of our protein using a gel shift.
TAL3 (BBa_K2915285)
The TAL3 gene is a binding protein for the amplicon. We've used the protein sequence to optimize its synthesis in E.coli. The part RFC 10 standards were used. We've usedBBa_K525998 as a RBS and T7 promoter that are inducible by IPTG. We have not tested the production and the activity of our protein.