Team:Aix-Marseille/Basic Part
Antifreeze proteins (BBa_K2915100)
Antifreeze proteins (AFPs) or ice structuring proteins (ISPs) are a class of polypeptides produced by animals, plants and microorganisms. They promote survival in subzero environments. We used them to avoid the destruction of our designed fusions proteins and to limit the formation of cristal water into the sample. These proteins can bring about the conservation of or fusions proteins. We used IDT tool to optimize to synthesis these proteins with E. coli. This part uses the RFC 10/25 standard.
NlaIII-Methylase (BBa_K2915300)
NlaIII gene code for a Methylase, which is use to recognized and methylated the targeted DNA. This methylase can recognized the site CATG. In order to identify the targeted DNa (amylase gene), the NlaIII-CBD was made as BBa_K2915300. We used the protein sequence to optimize synthesis by E. coli with IDT tool. This part uses the RFC 10/25 standard.
HhaI-Methylasse (BBa_K2915350)
The HhaI- gene code for a Methylase, which is use to recognized and methylated the targeted DNA. This methylase can recognized the site GCGC . In order to identify the targeted DNa ( Rpob and KatG gene), the HhaI-CBD was made as BBa_K2915350. We used the protein sequence to optimize synthesis by E. coli with IDT tool. This part uses the RFC 10/25 standard.
TolA-CBD (BBa_K2915400)
The TolA3 gene codes for the C-terminal domain located in the inner membrane of a bacterium. The third domain of this protein, called TolAE.coli. This protein coresponding is part of the cell divison process of the bacteria. In order to analyse the fixation of the P3-N1-chromoprotein fusion into the cellulose membrane, the TolA3-CBD was made as BBa_K2915400. We used the protein sequence to optimize synthesis by E. coli with IDT tool. This part uses the RFC 10/25 standard.
P3-N1-Green chromoprotein (BBa_K2915500)
The g3p gene code for the minor coat protein, which is located at the distal end of the M13 phage. The third domaine of this protein, called P3-N1CTX. This domain coresponding to the N-terminal domaine of P3 CTX protein and is part of the parasitage process of the M13 phage. In order to analyse the fixation of the chromoprotein into the cellulose membrane, the P3-N1-Green chromoprotein was made as BBa_K2915500. We used the protein sequence to optimize synthesis by E. coli with IDT tool. This part uses the RFC 10/25 standard.