Team:FAFU-CHINA/Model
OVERVIEW
In order to determine the specific number of days of cultivation in the industrial production process, we modeling about the effects of nitrogen and phosphorus on the biomass and lipids of C. reinhardtii. Finally, we used the model to determine the maximum number of days of harvesting nitrogen and phosphorus in the C. reinhardtii medium when the lipid content of C. reinhardtii was highest.
In the improvement part, we use the new BBa_K2935073 to improve the flocculation ability of the original BBa_K2407004 .Through protein structure analysis and prediction, we proved that the protein structure of the new partBBa_K2935073 has a very similar functional segment to that of the original BBa_K2407004 part, and also analyzed the protein structure of FLO5( BBA_K2935074).
1.Algal growth
We use the Logistic equation[1] to construct the growth kinetics of C. reinhardtii. The Logistic equation is:
For further sequence Alignment details, please click Sequence Alignment .
3.2Physicochemical property
We used the ProtParam (https://web.expasy.org/protparam/) to predict several kinds of Physicochemical property of these protein which contains amino acids, molecular weight, theoretical pI, amino acid composition, total number of positively/negatively charged residues, amino acid composition, formula, total number of atoms, extinction coefficients, instability index, aliphatic index and grand average of hydropathicity(GRAVY).
For further details, please click Physicochemical property.
the flocculation function as the flocculin FLO1 (BBa_K2407004).
3.5 Protein spatial structure
3.5.1 Secondary protein structure
We used PORTER to build up the protein’s secondary protein structure.For further details, please click Secondary protein structure.
