Team:FAFU-CHINA/Improve
OVERVIEW
After visiting Furiqing Algae Company, in order to solve the problem of algae loss encountered in their production, We designed the experiment through the self-flocculation device of Chlamydomonas reinhardtii, and the algae cells aggregated into a larger molecular weight algae complex to achieve the purpose of reducing the economic loss caused by the size of the mesh in the industrial production process.In order to achieve this goal, we found the part BBa_K2407004 ，but the sequence does not meet all the part standards, because this component needs to The other parts are used together, so there will be problems during the assembly process, which will make them unusable, and our team is also facing this problem.
METHODS
In order to solve this problem, we constructed BBa_K2935073 to enhance the flocculation ability of the original part BBa_K2407004, eliminate the problems in the assembly process and design the composite parts to regulate it, so that it can express flocculation function in the stable period of algae.
RESULTS AND DISCUSSIONS
After the Chlamydomonas reinhardtii enters the stationary phase, we irradiate the algae with blue light to excite the expression of the part BBa_K2935073. At the same time, we also transferred the original part BBa_K2407004 into the algae. The results are shown in Fig.2- Fig.5
CONCLUSIONS
1) We eliminated all restriction sites that did not meet the part standards and eliminated problems during assembly. 2) Design the composite part (BBa_K2935909) to regulate it to express the flocculation function during the stable period of the algae. 3) The part BBa_K2935073 we built enhances the flocculation ability of the original part BBa_K2407004.
