Team:Grenoble-Alpes/Parts

NeuroDrop

Parts

Parts that Team Grenoble-Alpes 2019 worked on.
BioBricks present in the final NeuroDrop system :
BBa_K3128019 : T18 Biosensor
BBa_K3128020 : T25 Biosensor
BBa_K3128001 : Strong Reporter

Significance in the project Purpose Name Type Description Designer Lenght (bp) RFC compatible
+++
 Biosensor for the mBATCH system BBa_K3128019 Composite COMP fused with T18 subpart of Bordetella Pertussis AC under constitutive promoter Lucas PINERO 1354 [10][23][1000]
+++
 Biosensor for the mBATCH system BBa_K3128020 Composite COMP fused with T25 subpart of Bordetella Pertussis AC under constitutive promoter Lucas PINERO 1486 [10][23][25][1000]
+++

 Reporter for our mBATCH system BBa_K3128001 Reporter NanoLuciferase reporter for BACTH assay Lucas PINERO 879 [10][12][21][23][25][1000]
+ To insert NanoLuciferase into other Biobrick BBa_K3128002 Coding NanoLuciferase with restrictions sites : BglII and BamHI Lucas PINERO 546 [10][12][23][25][1000]
+ To be able to change the reporter protein BBa_K3128004 Reporter NanoLuciferase reporter for BACTH assay (with two restriction enzymes around the reporter) Lucas PINERO 911 [10][12][23][25][1000]
- To be able to change the reporter protein BBa_K3128008 Coding Engineered mutant of red fluorescent protein with RFC21 restriction sites Lucas PINERO 690 [10][12][23][1000]
- BBa_K3128009 Reporter RFP protein under PLac promoter (with two restriction sites around the reporter) Lucas PINERO 1055 [10][12][23][1000]
- BBa_K3128011 Intermediate OmpX-WT fused with T18 subpart of Bordetella Pertussis adenylate cyclase under lactose promoter Melodie Dupre 1558 [10][12][23][1000]
- BBa_K3128012 Intermediate OmpX-WT fused with T25 subpart of Bordetella Pertussis adenylate cyclase under lactose promoter Melodie Dupre 1690 [10][12][23][25][1000]
- BBa_K3128015 Intermediate COMP fused with T18 subpart of Bordetella Pertussis adenylate cyclase under lactose promoter Melodie Dupre 1519 [10][12][23][1000]
- BBa_K3128016 Intermediate COMP fused with T25 subpart of Bordetella Pertussis adenylate cyclase under lactose promoter Melodie Dupre 1651 [10][12][23][25][1000]
++ Characterization of the mBATCH system BBa_K3128017 Composite OmpX-WT protein fused with T18 subpart of Bordetella Pertussis AC under constitutive promoter Lucas PINERO 1393 [10][23][1000]
++ Characterization of the mBATCH system BBa_K3128018 Composite OmpX-WT protein fused with T25 subpart of Bordetella Pertussis AC under constitutive promoter Lucas PINERO 1525 [10][23][25][1000]
- BBa_K3128022 Composite OmpX-WT with Leucine Zipper and T18 subpart of Bordetella Pertussis AC under lactose promoter Lucas PINERO 1686 [10][12][23]
- BBa_K3128023 Composite OmpX-WT with Leucine Zipper and T25 subpart of Bordetella Pertussis AC under lactose promoter Lucas PINERO 1818 [10][12][23][25]
- BBa_K3128024 Composite COMP fused with T18 subpart of Bordetella Pertussis AC under lactose promoter Melodie Dupre 1490 [10][21]
- BBa_K3128025 Composite COMP fused with T25 subpart of Bordetella Pertussis AC under lactose promoter Melodie Dupre 1622 [10][23][25]
++ Characterization of the mBATCH system BBa_K3128026 Composite OmpX-WT fused with Leucine Zipper and T18 subpart of B.Pertussis AC under constitutive promoter Lucas PINERO 1521 [10][21]
++ Characterization of the mBATCH system BBa_K3128027 Composite OmpX-WT fused with Leucine Zipper and T25 subpart of B.Pertussis AC under constitutive promoter Lucas PINERO 1622 [10][23][25]
++ Characterization of the mBATCH system BBa_K3128028 Composite COMP fused with Leucine Zipper and T18 subpart of B.Pertussis AC under constitutive promoter Lucas PINERO 1490 [10][21]
++ Characterization of the mBATCH system BBa_K3128029 Composite COMP fused with Leucine Zipper and T18 subpart of B.Pertussis AC under constitutive promoter Lucas PINERO 1622 [10][23][25]
++ Characterization of the mBATCH system BBa_K3128031 Coding OmpX-WT gene Melodie Dupre 513 [10][12][21][23][25][1000]
+++ Parts use in the final Biosensor Biobrick BBa_K3128032 Coding COMP : Clickable OmpX Fahd TIBOURTINE 513 [10][12][21][23][25][1000]
++ Characterization of the mBATCH system BBa_K3128033 Composite COMP under constitutive promoter Fahd TIBOURTINE 623 [10][21][23][25][1000]
++ Characterization of the mBATCH system BBa_K3128050 Coding Signal peptide from OmpX Lucas PINERO 69 [10][12][21][23][25][1000]
+ Characterization of the mBATCH system BBa_K3128021 Coding Leucine Zipper Melodie Dupre 120 [10][12][21][23][25]
+++ Parts use in the final Biosensor Biobrick BBa_K3128010 Coding Flexible Glycine Glycine Serine (GGS) Linker (54 aa) Melodie Dupre 162 [10][12][21][23][25][1000]
++ Characterization of the mBATCH system BBa_K3128025 Coding OmpX gene without signal peptide Lucas PINERO 444 [10][12][21][23][25][1000]