Team:Grenoble-Alpes/Attributions

WET LAB

TEAM MEMBERS

Lucas worked on the biological aspects and contributed to bibliographic search, to the design of the biobricks and did biological experiments including cloning and characterization of the Biobricks.

Marine worked on the characterization of the Biobrick BBa_J04450 and the bioluminescent reporter gene with Lucas. She wrote the safety form. She organized some events and was in charge of the goodies. She participated in fundraising.

Mélodie worked with Lucas on the gold medal criterion by improving biobricks in order to create a BACterial Two Hybrid (BATCH) (design in silico, cloning, bioluminescence assays). She participated in fundraising and relationships with sponsors. She organized and was part to a lots of events and was in charge of the project presentation slides.

Marvin worked on the bacterial two hybrid with Mélodie.

Fahd worked on the bibliographic search, implementation of protocols and cloning design in silico. He was in charge of the choice of the expression system. Fahd participated in the search for sponsors and the writting of bidding documents to raise funds. He had the opportunity to present the team during the different meetings.

Pauline worked on the whole aptamer part (bibliography, choice of sequences, characterization). She helped Fahd on its part and made tear samples for Maurice Mikkers's photos.

SPECIAL THANKS TO

Dr. Pierre Cavailles, for his priceless assistance with the FACS. He helped us with every experiments, and he supported us whenever he had the time to.

Dr Claire Durmort, who allowed us to have access to the microscope within the laboratory of the IBS. She helped us for the preparation of the samples but especially to make the focus stacking of the microscope.And for and her precious help to the proofreading of parts and files.

Dr Audrey Le Gouellec, for her advise in the construction of the project and proofreading some documents of the WIKI (ideas with BACTH for instance).

Clément Caffaratti, for his time and help on the overall project.

Dr Corinne Ravelet, for her expertise on aptamers, her constant help during experiments and the access she gave us to the locals and material of the “Département de Pharmacochimie Moléculaire”.

Dr Kevin Pounot, for his expertise on alpha-synuclein and Tau proteins, and his huge help for the acquisition of samples of these proteins.

Dr Guillaume Tetreau, for his warm welcome at the Institute of Structural Biology, his help for the plasmid construction and his expertise on the Gibson cloning.

Also, we would like to thank the whole TP team of Jean Roget and especially Sophie who assured we had good work conditions during the whole duration of the project.

DRY LAB

TEAM MEMBERS

Pierre worked on the interface between man and machine for our device, as well as the software to create and implement protocols for the device. He also worked on most of the parts of the hardware, especially on the theory side, to analyze how it should work, and how to command everything from his computer.

Romain was the beta tester for all the software created by Pierre. His time was heavily taken by the conception and debugging of the whole hardware, as he took an interest in every part of it as well as in the global conception of the device. He was also present on the mechanical production side, along with Antoine, and presented the project during the event "Midi Minatech".

Hajar took the entire work of designing the modeling of our biological system. She also worked on almost every part of the device, especially on the temperature control part. She was also in charge of the medical device regulation and the solutions for sterilization and biosafety of the device.

Antoine rebuilt the entire device in a 3D CAO software before any assembling. He was responsible of the global design and the mechanicals conception of the device. He also worked on the other part of the hardware, especially on the differents electronics cards needed for the device.

SPECIAL THANKS TO

Antoine Pisa for his time invested in the production of all of our PCB test cards.

Julien Traveaux for his help on the mechanical design and production of the device.

Félix Piédallu for his advice on the electronics as well as his help in the mechanical conception of the device.

Nicolas Ruty for his help on the electronic design and his critical mind on our solutions.

Delphine Ropers for her help with the creation of our simple bio-informatic model.

HUMAN PRACTICES

TEAM MEMBERS

Aline directed and realized interviews on biological, technical, legal, ethical, commercial, industrial and medical aspects of the project, to assess its viability, integration and legitimity in these fields.

Nina worked on the market study for our project and hired the community members for our online community that she animated and used as a sources for analysis. She also worked on risks analysis and the final SWOT.

SPECIAL THANKS TO

Elena Moro, Audrey Le Gouellec, Corinne Ravelet, Isabelle Pelletier, Mélanie Marcel, Thomas Landrain, Juliette Verseux, Arnaud Le Tiec, Pauline Armand, Yves Fouillet, Anne-Marie Benoit and Thierry Bontems for their contribution to the project.

Finally, thanks to Reia Anquet, Nordine Hocine and Severine Louvel for their feedback and corrections.

WIKI

Gaspard was responsible for our wiki. He created and implemented the whole structure, design and coding of it, methodically uploading our results and explanations up to the last moment.

COLLABORATIONS & PARTENERSHIPS

Our team realised two collaborations and one partnership this year, thanks to Romain, Hajar, Aline, Nina, Antoine and Fahd, and their presence in several meetings and places to meet our partners and co-iGEMers, such as the French meeting held in Poitiers or the European meeting in Paris.

For theses collaborations and partnership, we would like to thanks :
Maurice Mikkers, for his time and his beautiful pictures of crystallized tears. We got to meet him in Amsterdam to make this work, and we are glad of the result. We were also able to help him in his experimentations.
Marc Santoliniand all his team from the CRI. They collaborated with us on iGEM TIES. Lisa Bourne from the iGEM Nantes team with who we planned our collaboration with them.

SPONSORING

The sponsoring was also a huge part of the work we did on this project, as we raised our funds by ourselves. Once again, all the team was involved and spent time in meeting, budget attributions councils and administrations councils in banks, institutions and companies, to collect the funds we needed. On this particular topic, we would like to thanks Gabriel Soleil from Grenoble-INP student’s desk, and Murielle Brachotte from the Grenoble-INP foundation and Nathalie Martino,Mélanie Santailler and Paola Gardellin from the University Grenoble Alps Foundation for their help and constant support.

We also want to thank all of our sponsors and support, who believed in us and in our future achievements. Find out more about them on our sponsor page

COMMUNICATION

Due to the fact that we had to raise our funds by ourselves, we had to make a lot of communication around what we did as a team. In that objectives, all of the team members helped in a way or another for the communication of the team. We were particularly involved in social medias and performed well on facebook, youtube and instagram for example.

On this last one we would like to thank Aymeric Morlé, Stéphanie Grisel and Darcia Schweitzer from Promega, for allowing us to a takeover on the Promega instagram account for one week.
On theses different medias, we were involved in raising the interest for synthetic biology as well as explaining what we do as an iGEM team.

We also succeeded, thanks to the hard work of Melodie, Marvin and Antoine, and the voices of Nina and Hajar, to the realization and publication of 3 different movies, promoting iGEM and our team as well as explaining our work and the theory behind it.

EVENTS AND MEETINGS

In parallel with our massive media presence, we had to be there for a lot of conventions, meeting and events among which the “Fêtes de la Science” in Grenoble (science festival), the “Parvis des sciences” (an other science festival), the international Parkinson Day convention, the Biotechnologies Forum, the Medical Research day and the EXON’s day.

We also organized a conference explaining our project and what is at stakes with it for a “Midi Minatech”,in front of more than 150 high school students (picture).
For this we would like to thank all the staff of these events, and all our numerous contact in these organisations for their help and kindness.