Overview
This year we generated a vast collection of parts which can be divided into two subgroups. On the one hand parts related to our kill switch system consisting of the CRISPR/Cas3 system and its regulators and on the other hand our collection of parts involved in the evaluation of different cell-penetrating peptides (CPP).
Our contributions to the iGEM registry can be used for diverse applications and will be beneficial for future projects. Not only did we contribute numerous valuable new parts, but we also combined existing features with our new parts. Our Cas3 part represents an important contribution to the collection, because CRISPR-technologies are researched worldwide. Furthermore, our CRISPR/Cas3-based kill-switch offers a new approach towards biocontainment and biosafety. It is unique in the registry, which fosters cutting edge science within iGEM. Our three regulatory systems allow for controlled expression of any gene and can be easily implemented into other projects. Additionally, our cell-penetrating peptide (CPP) collection contributes to the registry’s great diversity and provides a variety of functionally related parts to gain the best results. The great diversity and extent of our part collection clearly enriches the iGEM registry and will be of great benefit for future projects.
The individual parts of our collection are listed below and already divided into two subgroups.
Individual Parts
| Collection | Name |
|---|---|
| CPP | BBa_K3096019 6x His Tag |
| CPP | BBa_K3096013 Penetratin - a cell penetrating peptide |
| CPP | BBa_K3096010 YTA2 - cell penetrating peptide |
| CPP | BBa_K3096015 pVEC - a cell penetrating peptide |
| CPP | BBa_K3096016 Tat-LK15 - improved Cell penetrating peptide |
| CPP | BBa_K3096017 SynB1 - cell penetrating peptide |
| CPP | BBa_K3096023 GFP with linkers for fusion with His Tag and CPPs |
| CPP | BBa_K3096026 YTA2 with His-tag and GFP |
| CPP | BBa_K3096029 Tp10 Transportan 10 with His-tag and GFP |
| CPP | BBa_K3096035 SynB1 with His-tag and GFP |
| CPP | BBa_K3096036 pVec with His-tag and GFP |
| CPP | BBa_K3096037 Penetratin with His-tag and GFP |
| CPP | BBa_K3096038 Tat-LK15 with His-tag and GFP |
| Kill Switch | BBa_K3096002 RBS-AraC-Term |
| Kill Switch | BBa_K3096005 constitutive FadR expression and regulated promoter |
| Kill Switch | BBa_K3096008 NagC regulated Mnt repressor |
| Kill Switch | BBa_K3096020 Constitutive nagC expression |
| Kill Switch | BBa_K3096025 Mnt-RFP reporter for Mnt expression |
| Kill Switch | BBa_K3096040 Temperature responsive repression system |
| Kill Switch | BBa_K3096046 Fatty acid responsive repression system |
| Kill Switch | BBa_K3096048 N-Acetyl-Glucosamin-6 phosphate regulated repression system |
| Kill Switch | BBa_K3096051 CRISPR array that targets the genomic DNA of E. coli - expression controlled via the Mnt repressor |
| Kill Switch | BBa_K3096053 CRISPR Array targeting the Plasmid DNA of the Exendin-4 expression system controlled by Lsr |
| Kill Switch | BBa_K3096054 Cas3 Nuclease under the control of the AraC promoter pBAD |
| Kill Switch | BBa_K3096055 Type I CRISPR/Cas system surveillance complex Cascade ABCDE under the control of the AraC promoter |
| Kill Switch | BBa_K3096001 The Cas3 Nuclease is part of the Type I CRISPR/Cas system in E. coli |
| Kill Switch | BBa_K3096006 NagC regulated promoter |
| Kill Switch | BBa_K3096050 Mnt |
| Kill Switch | BBa_K3096014 CRISPR Array targeting Genomic E. coli DNA |
| Kill Switch | BBa_K3096052 CRISPR Array targetting the Plasmid DNA of the glucose dependent |
| Kill Switch | BBa_K3096007 Type I CRISPR/Cas system surveillance complex Cascade ABCDE |
| Kill Switch | BBa_K3096018 NagC repressor |
