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Team:Tuebingen/Basic Part

GLP.exe - Basic Parts

Basic Parts

Overview

Our best basic part is the coding sequence of the Cas3 Nuclease (BBa_K3096001), which is part of the Type 1 CRISPR/Cas adaptive system in E. coli. Unlike Cas9-based CRISPR systems, our system does not only cut, but also degrade the DNA targeted by the CRISPR arrays. We have amplified this part via PCR from genomic DNA of E. coli MG1655 and confirmed it via gel electrophoresis. With this part, we newly introduce the Cas3 nuclease to the iGEM registry. It is a nuclease bearing a lot of potential regarding biosafety and the knock-out of genes. For further information, please visit our Kill Switch page (Project/Kill Switch) and the iGEM registry of BBa_K3096001.

Basic Parts

Table 1: Our basic parts.
Parts/Name Type Collection Length [bp]
BBa_K3096001
The Cas3 Nuclease is part of the Type I CRISPR/Cas system in E. coli
Coding Kill Switch 2667
BBa_K3096000
RBS
RBS 15
BBa_K3096006
NagC regulated promoter
Regulatory Kill Switch 200
BBa_K3096011
CAP promoter without LacI binding site
Regulatory Exendin-4 expression system 183
BBa_K3096050
Mnt
Regulatory Kill Switch 90
BBa_K3096014
CRISPR Array targeting Genomic E. coli DNA
RNA Kill Switch 206
BBa_K3096052
CRISPR Array targetting the Plasmid DNA of the glucose dependent Exendin-4 expression system
RNA Kill Switch 147
BBa_K3096019
6x His Tag
Tag CPP 18
BBa_K3096007
Type I CRISPR/Cas system surveillance complex Cascade ABCDE
Coding Kill Switch 4351
BBa_K3096013
Penetratin - a cell penetrating peptide
Coding CPP 48
BBa_K3096010
YTA2 - cell penetrating peptide
Coding CPP 48
BBa_K3096015
pVEC - a cell penetrating peptide
Coding CPP 54
BBa_K3096016
Tat-LK15 - improved Cell penetrating peptide
Coding CPP 78
BBa_K3096017
SynB1 - cell penetrating peptide
Coding CPP 54
BBa_K3096018
NagC repressor
Coding Kill Switch 1373
BBa_K3096023
GFP with linkers for fusion with His Tag and CPPs
Coding CPP 726
BBa_K3096030
Exendin-4 (GLP-1 analogue) with secretion signal
Coding Exendin-4 expression system 126