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Team:Tuebingen/Composite Part

GLP.exe - Composite Parts

Composite Part

Best composite part

Our best composite part is BBa_K3096042, which encodes for Exendin-4 with an N-terminal secretion tag and a C-terminal cell penetrating peptide. In total, this part allows for the expression, secretion and uptake of Exendin-4 into the circulatory system and thus represents our fully functional peptide-based therapeutic.Its activity can be confirmed via cell culture assays.

For further information, please visit our subpage about Incretins (Project/Incretin) and the parts registry of BBa_K3096042.

Table 1: Our composite parts.
Name Collection Composite Length [bp]
BBa_K3096042
Exendin-4 with a N-terminal secretion tag and a C-terminal cell penetrating peptide
Exendin-4 secretion system BBa_K3096000
BBa_K1012004
BBa_K3096030
BBa_K3096013
BBa_B0014
390
BBa_K3096002
RBS-AraC-Term
Kill Switch BBa_K3096000
BBa_K1088005
BBa_B0014
1003
BBa_K3096005
constitutive FadR expression and regulated promoter
Kill Switch BBa_K861052
BBa_K861060
BBa_K3096000
994
BBa_K3096008
NagC regulated Mnt repressor
Kill Switch BBa_K3096006
BBa_C0072
BBa_B0014
622
BBa_K3096012
Glucose-dependent TetR expression
Exendin-4 secretion system BBa_K3096011
BBa_K3096000
BBa_C0040
BBa_B0014
1000
BBa_K3096020
Constitutive nagC expression
Kill Switch BBa_J23100
BBa_K3096000
BBa_K3096006
BBa_B0014
369
BBa_K3096025
Mnt-RFP reporter for Mnt expression
Kill Switch BBa_K608351
BBa_K3096000
BBa_K3096030
1843
BBa_K3096026
YTA2 with His-tag and GFP
CPP BBa_K3096019
BBa_K3096023
BBa_K3096010
808
BBa_K3096029
Tp10 Transportan 10 with His-tag and GFP
CPP BBa_K3096019
BBa_K3096023
BBa_K380004
823
BBa_K3096035
SynB1 with His-tag and GFP
CPP BBa_K3096019
BBa_K3096023
BBa_K3096017
814
BBa_K3096036
pVec with His-tag and GFP
CPP BBa_K3096019
BBa_K3096023
BBa_K3096015
814
BBa_K3096037
Penetratin with His-tag and GFP
CPP BBa_K3096019
BBa_K3096023
BBa_K3096013
809
BBa_K3096038
Tat-LK15 with His-tag and GFP
CPP BBa_K3096019
BBa_K3096023
BBa_K3096016
838
BBa_K3096040
Temperature responsive repression system
Kill Switch BBa_K608351
BBa_K3096002
1959
BBa_K3096044
Glucose dependent expression system of Exendin-4 with a N-terminal secretion tag and a CPP
Exendin-4 secretion system BBa_K3096012
BBa_K3096045
1311
BBa_K3096045
Tet prom. with Exendin-4 with a N-terminal secretion tag and a C-terminal cell penetrating peptide
Exendin-4 secretion system BBa_R0040
BBa_K3096042
452
BBa_K3096046
Fatty acid responsive repression system
Kill Switch BBa_K3096005
BBa_K091001
1945
BBa_K3096048
N-Acetyl-Glucosamin-6 phosphate regulated repression system
Kill Switch BBa_K3096020
BBa_K3096008
999
BBa_K3096051
CRISPR array that targets the genomic DNA of E. coli - expression controlled via the Mnt repressor
Kill Switch BBa_K3096050
BBa_K3096014
304
BBa_K3096053
CRISPR Array targeting the Plasmid DNA of the Exendin-4 expression system controlled by Lsr
Kill Switch BBa_K117002
BBa_K3096052
257
BBa_K3096054
Cas3 Nuclease under the control of the AraC promoter pBAD
Kill Switch BBa_I13453
BBa_K3096001
2803
BBa_K3096055
Type I CRISPR/Cas system surveillance complex Cascade ABCDE under the control of the AraC promoter
Kill Switch BBa_I13453
BBa_K3096007
4487