Team:Tuebingen/Composite Part

GLP.exe - Composite Parts

Best composite part

Our best composite part is BBa_K3096042, which encodes for Exendin-4 with an N-terminal secretion tag and a C-terminal cell penetrating peptide. In total, this part allows for the expression, secretion and uptake of Exendin-4 into the circulatory system and thus represents our fully functional peptide-based therapeutic.Its activity can be confirmed via cell culture assays.

For further information, please visit our subpage about Incretins (Project/Incretin) and the parts registry of BBa_K3096042.

Table 1: Our composite parts.
Name	Collection	Composite	Length [bp]
BBa_K3096042 Exendin-4 with a N-terminal secretion tag and a C-terminal cell penetrating peptide	Exendin-4 secretion system	BBa_K3096000 BBa_K1012004 BBa_K3096030 BBa_K3096013 BBa_B0014	390
BBa_K3096002 RBS-AraC-Term	Kill Switch	BBa_K3096000 BBa_K1088005 BBa_B0014	1003
BBa_K3096005 constitutive FadR expression and regulated promoter	Kill Switch	BBa_K861052 BBa_K861060 BBa_K3096000	994
BBa_K3096008 NagC regulated Mnt repressor	Kill Switch	BBa_K3096006 BBa_C0072 BBa_B0014	622
BBa_K3096012 Glucose-dependent TetR expression	Exendin-4 secretion system	BBa_K3096011 BBa_K3096000 BBa_C0040 BBa_B0014	1000
BBa_K3096020 Constitutive nagC expression	Kill Switch	BBa_J23100 BBa_K3096000 BBa_K3096006 BBa_B0014	369
BBa_K3096025 Mnt-RFP reporter for Mnt expression	Kill Switch	BBa_K608351 BBa_K3096000 BBa_K3096030	1843
BBa_K3096026 YTA2 with His-tag and GFP	CPP	BBa_K3096019 BBa_K3096023 BBa_K3096010	808
BBa_K3096029 Tp10 Transportan 10 with His-tag and GFP	CPP	BBa_K3096019 BBa_K3096023 BBa_K380004	823
BBa_K3096035 SynB1 with His-tag and GFP	CPP	BBa_K3096019 BBa_K3096023 BBa_K3096017	814
BBa_K3096036 pVec with His-tag and GFP	CPP	BBa_K3096019 BBa_K3096023 BBa_K3096015	814
BBa_K3096037 Penetratin with His-tag and GFP	CPP	BBa_K3096019 BBa_K3096023 BBa_K3096013	809
BBa_K3096038 Tat-LK15 with His-tag and GFP	CPP	BBa_K3096019 BBa_K3096023 BBa_K3096016	838
BBa_K3096040 Temperature responsive repression system	Kill Switch	BBa_K608351 BBa_K3096002	1959
BBa_K3096044 Glucose dependent expression system of Exendin-4 with a N-terminal secretion tag and a CPP	Exendin-4 secretion system	BBa_K3096012 BBa_K3096045	1311
BBa_K3096045 Tet prom. with Exendin-4 with a N-terminal secretion tag and a C-terminal cell penetrating peptide	Exendin-4 secretion system	BBa_R0040 BBa_K3096042	452
BBa_K3096046 Fatty acid responsive repression system	Kill Switch	BBa_K3096005 BBa_K091001	1945
BBa_K3096048 N-Acetyl-Glucosamin-6 phosphate regulated repression system	Kill Switch	BBa_K3096020 BBa_K3096008	999
BBa_K3096051 CRISPR array that targets the genomic DNA of E. coli - expression controlled via the Mnt repressor	Kill Switch	BBa_K3096050 BBa_K3096014	304
BBa_K3096053 CRISPR Array targeting the Plasmid DNA of the Exendin-4 expression system controlled by Lsr	Kill Switch	BBa_K117002 BBa_K3096052	257
BBa_K3096054 Cas3 Nuclease under the control of the AraC promoter pBAD	Kill Switch	BBa_I13453 BBa_K3096001	2803
BBa_K3096055 Type I CRISPR/Cas system surveillance complex Cascade ABCDE under the control of the AraC promoter	Kill Switch	BBa_I13453 BBa_K3096007	4487