Team:GDSYZX/Parts

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Parts

Parts always play an important role in contributing to the development of Biosynthesis and can bring convenience of future researches. So this year, GDSYZX also attaches much importance to jobs concerning parts. We characterized a previous part(BBa_K763001) ; registered two new parts(BBa_K2947000,BBa_K2947001) and improved a frequently-used promoter(BBa_K1467101 →BBa_K2947002 ).

Part Number	Name	Type	Part Description
BBa_K2947000	HPAAS	Basic	4HPAAS(referring to 4-hydroxyphenylacetaldehyde synthase) protein coding region
BBa_K2947001	UGT33	Basic	UDP-glucose-4-O-glucosyltransferase(T8GT) protein coding region
BBa_K2947002	d35s	Basic	Double GoldenGate compatible 35S Promoter

Characterization

To add further experimental data to Part:BBa_K763001 for a others’ better using in the future,we run the enzyme digestion verification and sequence test of plasmid GroE-AsRed2, then test the fluorescence intensity under different heat-induce time. (set gradient between 0 and 30 minutes.)
BBa_K763001's page

New Parts/Validated Contribution

4HPAAS and UGT33 are the key genes for producing salidroside. In the case that the salidroside anabolism pathway in Arabidopsis has not been explored, we successfully predicted two key genes and obtained the expression of salidroside after transferring into Arabidopsis.These two parts, which are the main approaches to Arabidopsis Thaliana’s production of salidroside, is modified and proved functioning as expected.
4HPAAS's page                  UGT33's page

Improve

35S is a plant specific promoter obtained from the Cauliflower Mosaic Virus. The part is intended for use as a constitutive promoter for gene expression experiments in plants. We add two 35s promotor together to get a d35s promotor, then we merge GFP gene with d35s promotor to make a binary expression vector.
35s's page                  d35s's page