Attribution
Experiment | Human Practice | Art Design | Modeling | Wiki Construction | |
---|---|---|---|---|---|
Can Yang | ✔ | ✔ | |||
Shan Huang | ✔ | ✔ | |||
Ke Ruan | ✔ | ||||
Xinyi He | ✔ | ||||
Hongsheng Lin | ✔ | ||||
Wenkai Ding | ✔ | ||||
Zhanghao Wang | ✔ | ✔ | |||
Zhiheng Deng | ✔ | ||||
Kehui Zhang | ✔ | ✔ | |||
Xinyue Liu | ✔ | ✔ | |||
Xiongying Zheng | ✔ | ||||
Tengyuan Liu | ✔ | ✔ | |||
Yingjun Peng | ✔ | ✔ | |||
Hongzhe Wang | ✔ | ||||
Junkai Qian |
Introduction
When we started for our project, we are divided into five teams. Every team has their own purposen. Some of us worked in more than one group. Here’s the detail of our work.
Experiment
Experiment
Synthesis the gene 4HPAAS(1473bp) and UGT33(1497bp)
--vector Construction
--Enzyme-digestion of the vector PUC19-HA:Zhanghao Wang, Can yang, Zhiheng Deng, Wenkai Ding, Ke Ruan
--Clone the target segment 4HPAAS and RrUGT33:Zhanghao Wang, Canyang, Zhiheng Deng, Xingyi He, Hongzhe Wang
--Reuse the DNA gel:Zhanghao Wang, Canyang, Zhiheng Deng, Hongsheng Lin, Yingjun Peng
--The enzyme-digestion of the Target segment: Zhanghao Wang, Canyang, Zhiheng Deng, Shan Huang, Tengyuan Liu
--The ligation between the vector and target segment:Zhanghao Wang, Canyang, Zhiheng Deng, Kehui Zhang, Xiongying Zheng
--Transform vector into competent cell of Escherichia Coli:Zhanghao Wang, Canyang, Zhiheng Deng, Xinyue Liu, Ke Ruan
--Select the positive clone:Zhanghao Wang, Canyang, Zhiheng Deng
--Extract Plasmid:Zhanghao Wang, Canyang, Zhiheng Deng, Wenkai Ding, Kehui Zhang
--Prepare the protoplast of Arabidopsis:Zhanghao Wang, Canyang, Zhiheng Deng, Yingjun Peng, Xinyue Liu
--Transfection of the Arabidopsis’s protoplast:Zhanghao Wang, Canyang, Zhiheng Deng, Tengyuan Liu
--Western blot:Zhanghao Wang, Canyang, Zhiheng Deng
--Extracting and detecting the target segment
Get the gene which express the target segment:Zhanghao Wang, Canyang, Zhiheng Deng
Using alcohol solution to extract salidroside:Zhanghao Wang, Canyang, Zhiheng Deng
Using the liquid chromatography to detect the target product:Zhanghao Wang, Canyang, Zhiheng Deng
Human Practice
--Doing Online Survey: Shan Huang, Can Yang
--Leaning in Zhuhai: Shan Huang, Xingyue Liu, Tengyuan Liu
--Foshan Hospital and Underground Station:Ke Ruan, Shan Huang, Can Yang, Kehui Zhang, Wenkai Ding, Tengyuan Liu,Yingjun Peng
--Library: All of us
--School: Ke Ruan, Xingyi He, Can Yang, Zhanghao Wang, Hongsheng Lin
Art Design
--Wiki :Xinyue Liu, Xingyi He
--Poster:Xiongying Zheng
--Banner:Xingyue Liu
--PPT:Xingyue Liu
--Team Logo:Xingyue Liu
Modeling
Hongzhe Wang, Tengyuan Liu
Wiki
Shan Huang , Hongsheng Lin
Advisors
While we are working hard for our project, we also get lots of help from other teachers. Some of them introduced the project to us, while others taught specific topics to us.
Honglei Jin
Mr Jin is the PI of our team. He introduced the project of salidroside to us. He also did a lot of guidance for us.
Shuang Miao Dr Miao is the guiding teacher of us. She taught lots of useful knowledge to us. From her teaching, we understood the basic knowledge about salidroside. She taught us how to do the experiments. Our excellent words of experiment are also editted by her. Xinyu Zhang Yinpin Huang Shilin Zhu Weiteng Xu Kaiwen Zhou Jiahua Zou Jiadong Li Hui Luo Lanlan Guan
Shuang Miao Dr Miao is the guiding teacher of us. She taught lots of useful knowledge to us. From her teaching, we understood the basic knowledge about salidroside. She taught us how to do the experiments. Our excellent words of experiment are also editted by her. Xinyu Zhang Yinpin Huang Shilin Zhu Weiteng Xu Kaiwen Zhou Jiahua Zou Jiadong Li Hui Luo Lanlan Guan