Team:GDSYZX/Improve

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Improve

We add two 35s promotor together to get a d35s promotor, then we merge GFP gene with d35s promotor to make a binary expression vector. After that, we extract plasmids, transfer the plasmids into the protoplasts of Arabidopsis thaliana and culture the protoplasts for 12 hours to view fluorescence intensity run protein gel electrophoresis and Western blot to test the GFP expression rate.

Optimize the expression of GFP

The expression of protoplasts showed that the fluorescence intensity of GFP regulated by d35S promoter was stronger than that regulated by 35S promoter.

35s + GFP

d35s + GFP

The protoplast protein expression experiment showed that the expression of GFP under the regulation of d35S promoter was higher than that under the regulation of 35S promoter.