Difference between revisions of "Team:Marburg/Collaborations"

 
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       <h1 class="heading">
         COLLABORATION <!--  Titel austauschen-->
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         C O L L A B O R A T I O N S
 
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           Through this iGEM year we interacted with many iGEM Teams all around the world. It was a pleasure working
            
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          with all the different teams and learn from each other. We got many impressions about other projects and we
                Through this iGEM year we interacted with so many iGEM Teams all around the world. It was a pleasure working with
+
          hope that we could help with our collaborations. We would like to thank every team that collaborated with us!
              all the different teams and learn from each other. We got so many impressions about other projects and hope we could help with our collaborations.
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              A huge Thank you for every team collaboration !
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           onclick="popup('colony_pictures')">
 
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             <h1><!--  Titel von Pop up in schwarzer Box-->
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             <h1>
               Colony<br>
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               C O L O N Y<br>
            pictures
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              P I C T U R E S
 
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            <p>
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              <img src="https://static.igem.org/mediawiki/2019/f/f6/T--Marburg--ColonyPickingButton.svg">
                <!-- Untertitel im Popup -->           
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               <h1 class="title">Colony pictures</h1> <!--  Titel im pop up-->
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               <h1 class="title">The Colony Picture Collaboration</h1>
 
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               <section class="section"> <!--  Inhalt Pop up start-->
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               <section class="section">  
                <h2> <b> The Colony picture Collaboration </b></h2>
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                    <p>
+
 
                    <figure Style="float:left; margin-right: 25px;" >
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<div style="display: flex; flex-direction: row; align-items: center;">
                        <img style="height: 65ex; width: 50ex"
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                  <p><b>
                        src=https://static.igem.org/mediawiki/2019/5/5e/T--marburg--colony_picking_2.jpeg alt="Bild von Roboter Platzhalter">
+
                      In our colony picking collaboration we asked other iGEM teams to supply us with data for our
                        <figcaption>
+
                      picking algorithm.
                            Figure 1: Pipetting Robot (OT-2) colony picking set up.
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                      Thanks to this group effort we could build our Colony Picking Unit.</b>
                        </figcaption>
+
                        </figure>
+
                        Many repetitive tasks in the lab cost time such as pipetting or picking colonies from agar
+
                        plates and could
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                        technically be performed by robots. We used Opentrons OT-2 as a platform and designed hardware
+
                        modules and
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                        scripts to outsource these tasks. Thereby, we can now offer the Opentrons user community an
+
                        automated pipeline
+
                        to streamline laboratory practices in ways that haven’t been possible before.”
+
                   
+
                    </p>  </p>
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                    <br>
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                    <p> For the software we decided to use an artificial intelligence (AI). Artificial intelligences
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                        however rely on
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                        huge data sets to learn their specific task to perfection. In our case, we chose pictures of
+
                        agar plates with
+
                        colonies of E. coli as a suitable training set. Our experiments resulted in a big amount of agar
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                        plates but it
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                        was clear to us pretty quickly that we could never reach a big enough data set just by making
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                        photos of our own
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                        plates to get an AI with an adequate performance. To achieve this goal, we launched the Colony
+
                        picture
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                        collaboration. We asked other iGEM Teams if they could provide us pictures of their agar plates
+
                        with E. coli
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                        colonies with which we can then train the robot. In order to do that we published a script on
+
                        how to take the
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                        pictures. We made the script as precise as possible to make sure that the data set was as
+
                        consistent as
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                        possible.
+
 
                     </p>
 
                     </p>
                    <br> <br> <br> <br>
+
                 
                    <figure Style="text-align:center">
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<figure style="text-align: center; margin-left: 1em;">
                        <img style="height: 50ex; width: 75ex"
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                    <img src="https://static.igem.org/mediawiki/2019/f/f6/T--Marburg--ColonyPickingButton.svg"
                            src="https://static.igem.org/mediawiki/2019/f/f8/T--marburg--taking_colony_pictures.png"
+
                      alt="ColonyPickingButton"
                            alt="Pattern for taking the colony picture">
+
                      style="min-width: 200px;">
                        <figcaption>
+
                 
                            Figure 2: Pattern for taking the colony picture.
+
                  </figure>
                        </figcaption>
+
                </div>
                    </figure>
+
 
                    <br>
+
                <div style="display: flex; flex-direction: row;">
                     <p> Following this, we built a website so they could label the colonies on their images online.
+
                  <figure style="text-align: center; margin-right: 1em;">
 +
                    <img src=https://static.igem.org/mediawiki/2019/5/5e/T--marburg--colony_picking_2.jpeg
 +
                      alt="Pipetting Robot, colony picking set up"
 +
                      style="min-width: 350px;">
 +
                    <figcaption>
 +
                      Figure 1: Pipetting Robot (OT-2) colony picking set up.
 +
                    </figcaption>
 +
                  </figure>
 +
                  <div>
 +
                 
 +
                     <p>
 +
                      Many repetitive tasks in the lab cost time such as pipetting or picking colonies from agar plates
 +
                      and could technically be performed by robots.
 +
                      However, most commercially available solutions are simply too expensive and therefore unavailable
 +
                      for many Labs and iGEM Teams.
 +
                      So one of our goals was to build a reasonable colony picking robot to outsource the tiring task of
 +
                      colony picking by hand.
 
                     </p>
 
                     </p>
                     <br>
+
                     <p>
                     <figure Style="text-align:center">
+
                      We used an Opentrons OT-2, the cheapest available pipetting robot and constructed modules and
                        <img style="height: 50ex; width: 80ex"
+
                      scripts,
                            src="https://static.igem.org/mediawiki/2019/0/0b/T--marburg--collonycollab_website.png"
+
                      that allow other iGEM Teams to turn their own OT-2 into a colony picking robot as well.
                            alt="colony picking website">
+
                      See here how to transfer your OT2 into a
 +
                      <a style="padding: 0;"
 +
                        href="https://2019.igem.org/Team:Marburg/Colony_Picking"> Colony Picker.
 +
                      </a>
 +
 
 +
                     </p>
 +
                  </div>
 +
              </div>
 +
             
 +
<div style=" margin-top: 1em;">
 +
                  <p>
 +
                    We chose to build an Artificial Intelligence (AI) to automatically recognise colonies. While a “hard-coded”
 +
                    solution would be easier to achieve, they lack accuracy and flexibility, key attributes of Synbio projects.
 +
                    The drawback for an AI approach is the amount of data it needs for precise and robust training. 
 +
                    With the help of the iGEM-community we thought to overcome this problem. We asked other iGEM Teams 
 +
                    if they could provide us with labeled pictures of their agar plates with  <i>E. coli</i> colonies, which served as the
 +
                    basis for our AI. For that purpose we set up a
 +
                        <a style="padding: 0;"
 +
                        href="https://www.vinca.de/igem/"> Website.
 +
                      </a>
 +
</p>
 +
</div>
 +
               
 +
<div style="display: flex; flex-direction: row; margin-top: 1em;">
 +
                  <p>
 +
                    To participate all that was needed were agar plates with  <i>E. coli</i> colonies, a screen and a camera. Other
 +
                    criteria were evenly distributed, distinguishable colonies. The first step was taking the picture. For this
 +
                    purpose, the screen had to be set to the highest level of brightness and display a white image. When
 +
                    you take a picture of a common screen, a pattern on the picture could appear, the so called Moiré pattern.
 +
                    To avoid this, we supplied each team with diffusive sheets, which they could use to cover their screen.
 +
A detailed summary is found  <a style="padding: 0;"
 +
                        href="https://static.igem.org/mediawiki/2019/8/83/T--Marburg--Collaboration.pdf"> here.
 +
                      </a>
 +
 
 +
                  </p>
 +
                  <figure style="text-align: center; margin-left: 1em;">
 +
                    <img src="https://static.igem.org/mediawiki/2019/f/f8/T--marburg--taking_colony_pictures.png"
 +
                      alt="Pattern for taking the colony picture"
 +
                      style="min-width: 500px;">
 +
                    <figcaption>
 +
                      Figure 2: Pattern for taking the colony picture.
 +
                    </figcaption>
 +
                  </figure>
 +
                </div>
 +
 
 +
                <div style="display: flex; flex-direction: row; margin-top: 1em;">
 +
                  <figure style="text-align: center; margin-right: 1em;">
 +
                    <img src="https://static.igem.org/mediawiki/2019/0/0b/T--marburg--collonycollab_website.png"
 +
                      alt="colony picking website"
 +
                      style="min-width: 550px;">
 +
                    <figcaption>
 +
                      Figure 3: Website for colony picture labeling.
 +
                    </figcaption>
 +
                  </figure>
 +
                  <p>
 +
                    Following this, the images taken had to be labeled using a
 +
                  <a style="padding: 0;"href="https://www.vinca.de/igem/label/"> web-based
 +
                      </a>
 +
                  tool. Afterwards, the single colonies were marked with a rectangle.
 +
                    In total 250 colony pictures were added to our data set, vastly increasing the performance of our AI
 +
                    in detecting colonies. As an incentive to participate in this collaboration we gave away prizes to
 +
                    the teams that sent us their pictures.
 +
                  </p>
 +
                </div>
 +
                <div class="wrap-collabsible"
 +
                  style="margin-top: 25px;">
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                  <input id="Pizzes"
 +
                    class="toggle"
 +
                    type="checkbox">
 +
                  <label for="Pizzes"
 +
                    class="lbl-toggle">
 +
                    Participants and Prizes
 +
                    <hr style="width: unset;">
 +
                  </label>
 +
                  <div class="collapsible-content">
 +
                    <div class="content-inner"
 +
                      style="text-align: left; text-align-last: left;">
 +
                      <p style="margin-bottom: 25px;">
 +
                        As an incentive to participate in this Collaboration we gave away prizes. The five best teams
 +
                        that sent us pictures got an <i>E. coli</i> plushie and the three teams that sent us the most
 +
                        pictures got a bigger surprise. Thanks to Doulix, we provided these three teams with
 +
                        an engraved trophy out of glass and vouchers.
 +
             
 +
                      </p>
 +
                      <p>
 +
                        Team <a style="padding: 0;"
 +
                          href="https://2019.igem.org/Team:BOKU-Vienna"> BOKU-Vienna </a> was able to send us one
 +
                        picture, team <a style="padding: 0;"
 +
                          href="https://2019.igem.org/Team:GO_Paris-Saclay"> GO Paris-Saclay</a> provided three pictures
 +
                        and team <a style="padding: 0;"
 +
                          href="https://2019.igem.org/Team:TUDelft"> TUDelft</a> could help us with 14 colony
 +
                        pictures. Team <a style="padding: 0;"
 +
                          href="https://2019.igem.org/Team:TU_Darmstadt"> TU Darmstadt </a> could send us a number of
 +
                        23 pictures, but were beaten by team <a style="padding: 0;"
 +
                          href="https://2019.igem.org/Team:IISER_Bhopal"> IISER Bhopal </a> which send us an
 +
                        impressive amount of 99 pictures.
 +
                      </p>
 +
                      <figure style="text-align: center; margin-right: 1em;">
 +
                        <img src=https://static.igem.org/mediawiki/2019/0/04/T--Marburg--prizes_colony-picture.jpeg
 +
                          alt="Prizes colony picture">
 
                         <figcaption>
 
                         <figcaption>
                            Figure 3: Website for colony picutre labeling.
+
                          Figure 1: Giving out the prizes for the best participants of the Colony-Picture Project at
 +
                          the giant Jamboree.
 
                         </figcaption>
 
                         </figcaption>
                    </figure>
+
                      </figure>
                    <br>
+
                      <p>
                    <p> In total 250 colony pictures were added to our data set, vastly increasing the performance of
+
                         We thank all the teams for sending us labeled colony pictures. Without your help we would not
                         our AI in
+
                         have been able to train our AI and to transform our Opentrons OT-2 into a Colony Picker.
                        detecting colonies.
+
                      </p>
                        As an incentive to participate in this collaboration we gave away prizes to the teams that sent
+
                    </div>
                        us their
+
                  </div>
                        pictures, they received a small surprise, an E. coli plushie. The three teams that sent us the
+
                 </div>
                        most pictures got
+
               </section>
                         an engraved trophy out of glass thanks to our sponsor of this collaboration, Doulix.
+
                    </p>
+
                </p>  
+
                <br>
+
                 <br> <!--  Inhalt vom pop up, wenn nur Text dann <p>, wenn mit bilder etc extra ein <div> drum machen-->
+
               </section> <!--  Inhalt Pop up start-->
+
 
             </div>
 
             </div>
 
           </div>
 
           </div>
 
         </div>
 
         </div>
        <!--  neues pop nummer 2-->
+
         <div class="sub"
         <div class="sub"  
+
           onclick="popup('golden_gate')">
           onclick="popup('rbn2')">  <!--  nummerieren-->
+
 
           <div class="sub-header">
 
           <div class="sub-header">
             <h1>
+
             <h1>T H E &ensp; G O L D E N &ensp; G A
                    The Golden Gate <br> collaboration
+
              T E</h1>
             </h1>
+
             <h1>C O L L A B O R A T I O N</h1>
 
             <hr>
 
             <hr>
 
           </div>
 
           </div>
 
           <div class="sub-content">
 
           <div class="sub-content">
            <p>
+
            <img src="https://static.igem.org/mediawiki/2019/7/71/T--Marburg--GoldenGateCollabosButtons.svg">  
              <!-- kein text notwendig -->
+
            </p>
+
 
           </div>
 
           </div>
 
         </div>
 
         </div>
         <div id="rbn2"
+
         <div id="golden_gate"
           class="popup">  <!--  nummerieren-->
+
           class="popup">
 
           <div class="popup-container">
 
           <div class="popup-container">
 
             <div class="popup-header">
 
             <div class="popup-header">
 
               <h1 class="title">The Golden Gate collaboration</h1>
 
               <h1 class="title">The Golden Gate collaboration</h1>
 
               <button type="button"
 
               <button type="button"
                 onclick="hide('rbn2')">X</button> <!--  nummerieren-->
+
                 onclick="hide('golden_gate')">X</button>
 
             </div>
 
             </div>
            <div class="popup-content"
+
<div class="popup-content"
 
               style="text-align: justify;">
 
               style="text-align: justify;">
               <p>
+
               <section class="section">
                     Earlier this year the iGEM headquarters announced changes in their part submission system, one of
+
 
                    which made us extremely happy: BioBricks are now also accepted in Type IIS standard! In our opinion,
+
<div style="display: flex; flex-direction: row; align-items: center;">
                    this is a huge step, as cloning techniques based on Type IIS restriction enzymes are becoming more
+
                  <p><b>
                    and more popular in the scientific community due to their numerous advantages over classical cloning
+
                      Our team made itself the goal to introduce other iGEM Teams to Golden Gate.
                    methods.
+
                      Therefore we hosted a webinar, build up a communication platform
                    <br>
+
                      and made an Interlab study.</b>
                    We noticed that more teams converted to MoClo systems in their projects this year but we still think
+
                     </p>
                    there is a lot that needs to be done for the iGEM community to really embrace this change. In order
+
<figure style="text-align: center; margin-left: 1em;">
                    to help teams with the transition into this new system, we had an idea:
+
                    <img src="https://static.igem.org/mediawiki/2019/7/71/T--Marburg--GoldenGateCollabosButtons.svg"
                    What if we could help other teams understand what Type IIS enzymes really are and how they work? If
+
                      alt="GoldenGateCollabosButtons"
                    we could share our passion for Modular Cloning and the value of the PhytoBrick standard? If we could
+
                      style="min-width: 200px;">
                    introduce them to our Marburg Collection and help removing some of the burdens that come with a
+
                  </figure>
                    switch to a new cloning system?
+
                </div>
                    <br>
+
 
                    Therefore we hosted a webinar about Golden Gate, build up a communication platform to give iGEM
+
 
                    Teams a chance to troubleshoot their methods and made an Interlab study. Here we evaluated the
+
           
                    consistency of Golden Gate Assembly protocols across iGEM teams as well as to give teams the
+
                <p>
                    opportunity to gain experience with the assembly method.
+
                  Earlier this year the iGEM headquarters announced changes in their part submission system, one of
                    <br>
+
                  which made us extremely happy: BioBricks are now also accepted in Type IIS standard! In our opinion,
                    <br>
+
                  this is a huge step, as cloning techniques based on Type IIS restriction enzymes are becoming more
              </p>
+
                  and more popular in the scientific community due to their numerous advantages over classical cloning
               <br>
+
                  methods.<br>
               <br>
+
                  We noticed that more teams converted to MoClo systems in their projects this year but we still think
             
+
                  there is a lot that needs to be done for the iGEM community to really embrace this change. In order
                        <div class="wrap-collabsible">
+
                  to help teams with the transition into this new system, we had an idea:
                            <input id="collapsible2_1" class="toggle" type="checkbox">
+
                  What if we could help other teams understand what Type IIS enzymes really are and how they work? If
                            <label for="collapsible2_1" class="lbl-toggle">
+
                  we could share our passion for Modular Cloning and the value of the PhytoBrick standard? If we could
                                <h3 class="title">Webinar - Educating about Golden Gate cloning </h3>
+
                  introduce them to our Marburg Collection and help removing some of the burdens that come with a
                            </label>
+
                  switch to a new cloning system?<br>
                            <div class="collapsible-content">
+
                  Therefore we hosted a webinar about Golden Gate, build up a communication platform to give iGEM
                                <div class="content-inner" style="text-align: justify; text-align-last: left;">
+
                  Teams a chance to troubleshoot their methods and made an Interlab study. Here we evaluated the
                                    <p>
+
                  consistency of Golden Gate Assembly protocols across iGEM teams as well as to give teams the
                                    <br>
+
                  opportunity to gain experience with the assembly method.
                                        <figure Style="text-align:center"><!-- dynamisches bild-->
+
                </p>
                                            <img style="height: 50ex; width: 50ex"
+
               </section>
                                                src="https://static.igem.org/mediawiki/2019/0/01/T--marburg--Webinar_button.png" alt="Webinarbutton">
+
               <section class="section">
                                        </figure>
+
                <div class="wrap-collabsible"
                                        <br>
+
                  style="margin-bottom: 25px;">
                                        We wanted to reach as many teams as possible with our webinar on Golden Gate cloning,
+
                  <input id="collapsible2_1"
                                        Type IIS enzymes, their benefits and how to use all of this in their own projects. We
+
                    class="toggle"
                                        started planning everything that we thought should be in the webinar, from the
+
                    type="checkbox">
                                        biological function of the Type IIS enzymes and how they differ from Type II enzymes,
+
                  <label for="collapsible2_1"
                                        over its applications in Synthetic Biology like modular cloning to the in-depth
+
                    class="lbl-toggle">
                                        strategies when designing your own parts and actually building them in the lab.
+
                    Webinar - Educating about Golden Gate cloning
                                        <br>
+
 
                                        During this planning step we realized, that some of the teams that are already working
+
 
                                        with Golden Gate might not benefit as much from an entry level webinar. Consequently, we
+
                    <hr style="width: unset;">
                                        thought about integrating more complex design questions that we could share with the
+
                  </label>
                                        community. We finished our webinars with a practical part, showing the part-design in
+
                  <div class="collapsible-content">
                                        Geneious Prime – the sequence analysis software we used throughout the year. Firstly, we
+
                    <div class="content-inner"
                                        explained the different levels that can be built with Golden Gate and then introduced
+
                      style="text-align: justify; text-align-last: left;">
                                        some more complex designs that are also possible with Golden Gate cloning, such as the
+
                      <div style="display: flex; flex-direction: row;">
                                        construction of whole operons or space holder parts. Through this we hoped to also reach
+
 
                                        teams that use Golden Gate themselves, showing them the possibilities this cloning
+
                        <figure style="text-align: center; margin-right: 25px; min-width:200px">
                                        system offers.
+
                          <img src="https://static.igem.org/mediawiki/2019/3/37/T--Marburg--WebinarButton.svg"
                                        <br>
+
                            alt="Webinarbutton">
                                        To reach as many teams on the globe as possible we set up webinars on three different
+
                        </figure>
                                        times to cover as many time zones within a comfortable time frame – hoping that this
+
 
                                        will allow more iGEM teams to participate.
+
                        <p>
                                        The one and a half hour webinars were held on July 30th 10am CET, July 31st 6pm CET and
+
                          We wanted to reach as many teams as possible with our webinar on Golden Gate cloning,
                                        the last time on 8th August 12pm CET by our instructor René Inckemann with the support
+
                          Type IIS enzymes, their benefits and how to use all of this in their own projects. We
                                        of three of our team members.
+
                          started planning everything that we thought should be in the webinar, from the
                                        We were happy that in total 18 teams participated, as well as some students from the
+
                          biological function of the Type IIS enzymes and how they differ from Type II enzymes,
                                        universities of Marburg and Gießen and even a few fellow scientists from the
+
                          over its applications in Synthetic Biology like modular cloning to the in-depth
                                        Max-Planck-Institute for terrestrial microbiology. In total we counted around 70
+
                          strategies when designing your own parts and actually building them in the lab.<br> <br>
                                        participants. We were also really happy to see Sota Hirano from Doulix on our webinar,
+
                        </p>
                                        who gave us some awesome feedback, stating that he did learn new things about Golden
+
                        </div>
                                        Gate which he was not aware of before!
+
                        <p>
                                        We thank all the following teams for participating in this awesome event:
+
                          During this planning step we realized, that some of the teams that are already working
                                        <br>
+
                          with Golden Gate might not benefit as much from an entry level webinar. Consequently, we
                                        <br>
+
                          thought about integrating more complex design questions that we could share with the
                                        <a href="https://2019.igem.org/Team:Aachen/collaborations">Team Aachen</a> <br>
+
                          community. We finished our webinars with a practical part, showing the part-design in
                                        <a href="https://2019.igem.org/Team:Athens/Collaborations">Team Athens</a> <br>
+
                          Geneious Prime – the sequence analysis software we used throughout the year. Firstly, we
                                        <a href="https://2019.igem.org/Team:Amazonas-Brazil/Collaborations">Team
+
                          explained the different levels that can be built with Golden Gate and then introduced
                                            Amazonas-Brazil</a> <br>
+
                          some more complex designs that are also possible with Golden Gate cloning, such as the
                                        <a href="https://2019.igem.org/Team:CMUQ/Collaborations">Team CMU-Q</a> <br>
+
                          construction of whole operons or space holder parts. Through this we hoped to also reach
                                        <a href="https://2019.igem.org/Team:ITESO_Guadalajara/Collaborations">Team ITESO</a>
+
                          teams that use Golden Gate themselves, showing them the possibilities this cloning
                                        <br>
+
                          system offers.<br>
                                        <a href="https://2019.igem.org/Team:SoundBio//Collaborations">Team Soundbio</a> <br>
+
 
                                        <a href="https://2019.igem.org/Team:UFPR-Curitiba-Brazil/Collaborations">Team
+
                        </p>
                                            UFPR-Curitiba Brazil</a> <br>
+
                        <div style="display: flex; flex-direction: row; margin-top: 25px">
                                        <a href="https://2019.igem.org/Team:TUDelft/Collaborations">Team Delft</a> <br>
+
                   
                                        <a href="https://2019.igem.org/Team:BOKU-Vienna/Collaborations">Team BOKU-Viena</a> <br>
+
                          <p>
                                        <a href="https://2019.igem.org/Team:Duesseldorf/Collaborations">Team Düsseldorf</a> <br>
+
                          To reach as many teams on the globe as possible we set up webinars on three different
                                        <a href="https://2019.igem.org/Team:IISc-Bangalore/Collaborations">Team IlSc-BAngalore</a>
+
                          times to cover as many time zones within a comfortable time frame – hoping that this
                                        <br>
+
                          will allow more iGEM teams to participate.
                                        <a href="https://2019.igem.org/Team:TU_Kaiserslautern/Collaborations">Team Kaiserslautern</a>
+
                          The one and a half hour webinars were held on July 30th 10am CET, July 31st 6pm CET and
                                        <br>
+
                          the last time on 8th August 12pm CET by our instructor René Inckemann with the support
                                        <a href="https://2019.igem.org/Team:Leiden/Collaborations">Team Leiden</a> <br>
+
                          of three of our team members.
                                        <a href="https://2019.igem.org/Team:UM_Macau/Collaborations">Team Macau</a> <br>
+
                          We were happy that in total 18 teams participated, as well as some students from the
                                        <a href="https://2019.igem.org/Team:PuiChing_Macau/Collaborations">Team Macau</a> <br>
+
                          universities of Marburg and Gießen and even a few fellow scientists from the
                                        <a href="https://2019.igem.org/Team:Tartu_TUIT/Collaboration">FAU-Tartz-TUIT</a> <br>
+
                          Max-Planck-Institute for terrestrial microbiology. In total we counted around 70
                                        <a href="(https://2019.igem.org/Team:FAU_Erlangen/Collaborations">Team FAU-Erlangen</a>
+
                          participants. We were also really happy to see Sota Hirano from Doulix on our webinar,
                                        <br>
+
                          who gave us some awesome feedback, stating that he did learn new things about Golden
                                        <a href="https://2019.igem.org/Team:Queens_Canada/Collaborations">Team Quens-Canada </a>
+
                          Gate which he was not aware of before!
                                        <br>
+
                        </p>
                                        <br>
+
                        <figure style="text-align: center; margin-left: 25px; min-width:400px">
                                        <div>
+
                          <img src="https://static.igem.org/mediawiki/2019/f/f1/T--marburg--Doulix_webinar.jpeg"
                                        <figure Style="text-align:center"> <!-- Bild solte mittig sein -->
+
                            alt="Bilder Webinar"
                                            <img style="height:60ex; width:80ex"
+
                            style="max-height: 600px;">
                                                src="https://static.igem.org/mediawiki/2019/f/f1/T--marburg--Doulix_webinar.jpeg"
+
                          <figcaption>
                                                alt="Bilder Webinar">
+
                            Figure 3: Participants watching our Golden Gate webinar all over the world.
                                            <figcaption>
+
                          </figcaption>
                                                Figure 3: Participants watching our Golden Gate webinar all over the world.
+
                        </figure>
                                            </figcaption>
+
                      </div>
                                        </figure>  
+
 
                                    </div>
+
                                     
+
                                        <br>
+
                                        If you would like to learn about Golden Gate assembly please feel free to watch our
+
                                        webinar through this Link:
+
                                        <br>
+
                                        <a href="https://www.youtube.com/watch?v=-UUUG2iL7og">Webinar 30.07.2019 </a> <br>
+
                                        <a href="https://www.youtube.com/watch?v=Aq-6MlaVWww">Webinar 31.07.2019 </a> <br>
+
                                        <a href="https://www.youtube.com/watch?v=Aq-6MlaVWww">Webinar 09.08.2019 </a> <br>
+
                                    </p></div>
+
                            </div>
+
 
                          
 
                          
               
+
 
              <br>
+
                      <div style="display: flex; flex-direction: row; margin-top: 25px">
              <div class="wrap-collabsible">
+
                        <div>
                <input id="collapsible2_2"
+
                          <p>
                  class="toggle"
+
                            If you would like to learn about Golden Gate assembly please feel free to watch our
                  type="checkbox">
+
                            webinar through this Link:
                <label for="collapsible2_2"
+
                          </p>
                  class="lbl-toggle">
+
                          <div class="content">
                  <h3 class="title">Slack Channel and gDrive - Global networking in the Golden Gate community</h3>
+
                            <ul>
                </label>
+
                              <li><a href="https://www.youtube.com/watch?v=-UUUG2iL7og">Webinar 30.07.2019</a></li>
                <div class="collapsible-content">
+
                              <li><a href="https://www.youtube.com/watch?v=Aq-6MlaVWww">Webinar 31.07.2019</a></li>
                  <div class="content-inner"
+
                              <li><a href="https://www.youtube.com/watch?v=Aq-6MlaVWww">Webinar 09.08.2019</a></li>
                    style="text-align: left; text-align-last: left;">
+
                            </ul>
                    <div><p>
+
                          </div>
                            Although we were quite pleased with the result of our webinar, we thought about how to
+
<p>
                            further connect this Golden Gate community, so we created a Slack workspace for everyone
+
                          We thank all the following teams for participating in this awesome event:
                            that is working with this method, enabling us all to exchange protocols and ideas or
+
</p>
                            troubleshoot issues that might arise. So far there are close to 50 members, but we hope
+
                          <div class="content">
                            to find more enthusiasts willing to share their knowledge!<br>
+
                            <ul>
                            All files used for the webinar are openly available. You can find our protocols,
+
                              <li><a href="https://2019.igem.org/Team:Aachen/collaborations">Team Aachen</a></li>
                            handouts, slides as well as the files we used for the practical part in Geneious to
+
                              <li><a href="https://2019.igem.org/Team:Athens/Collaborations">Team Athens</a></li>
                            construct parts in-silico in our shared Google Drive. Through this we hope to make it
+
                              <li><a href="https://2019.igem.org/Team:Amazonas-Brazil/Collaborations">Team
                            easier for other teams willing to start with Golden Gate cloning, as they can start
+
                                  Amazonas-Brazil</a></li>
                            designing their first own in-silico parts with our guidance.
+
                              <li><a href="https://2019.igem.org/Team:CMUQ/Collaborations">Team CMU-Q</a></li>
                            <br> <br>
+
                              <li><a href="https://2019.igem.org/Team:ITESO_Guadalajara/Collaborations">Team ITESO</a>
                            The main documents of the Golgen Gate Webinar <br>
+
                              </li>
                            → Download: <a
+
                              <li><a href="https://2019.igem.org/Team:SoundBio//Collaborations">Team Soundbio</a></li>
                                href="https://static.igem.org/mediawiki/2019/0/0a/T--marburg--GG_webinar_slides.pdf">Golden
+
                              <li><a href="https://2019.igem.org/Team:UFPR-Curitiba-Brazil/Collaborations">Team
                                Gate webinar slide 2019 </a><br>
+
                                  UFPR-Curitiba Brazil</a></li>
                            → Download:<a
+
                              <li><a href="https://2019.igem.org/Team:TUDelft/Collaborations">Team Delft</a></li>
                                href="https://static.igem.org/mediawiki/2019/0/09/T--CMUQ--goldenGateWebinarHandout.pdf">Handout
+
                              <li><a href="https://2019.igem.org/Team:BOKU-Vienna/Collaborations">Team BOKU-Viena</a>
                                Golden Gate webinar </a> <br>
+
                              </li>
                            → Download:<a
+
                              <li><a href="https://2019.igem.org/Team:Duesseldorf/Collaborations">Team Düsseldorf</a>
                                href="https://static.igem.org/mediawiki/2019/c/c2/T--marburg--GG_protocoll_white.pdf">Golden
+
                              </li>
                                Gate protocoll </a>  
+
                              <li><a href="https://2019.igem.org/Team:IISc-Bangalore/Collaborations">Team
                                <br>
+
                                  IlSc-BAngalore</a>
                                <br>
+
                              </li>
                            To try out the described in-silico cloning you can download the needed sequences as
+
                              <li><a href="https://2019.igem.org/Team:TU_Kaiserslautern/Collaborations">Team
                            geneious file here. <br>
+
                                  Kaiserslautern</a></li>
                            → Download:<a
+
                              <li><a href="https://2019.igem.org/Team:Leiden/Collaborations">Team Leiden</a></li>
                                href="https://2019.igem.org/File:T--marburg--Sequences_Webinar.zip">Sequences
+
                              <li><a href="https://2019.igem.org/Team:UM_Macau/Collaborations">Team UM Macau</a></li>
                                Webinar </a> <br>
+
                              <li><a href="https://2019.igem.org/Team:PuiChing_Macau/Collaborations">Team Pui Ching
                  </p></div>  
+
                                  Macau</a></li>
 +
                              <li><a href="https://2019.igem.org/Team:Tartu_TUIT/Collaboration">Team FAU-Tartz-TUIT</a>
 +
                              </li>
 +
                              <li><a href="(https://2019.igem.org/Team:FAU_Erlangen/Collaborations">Team
 +
                                  FAU-Erlangen</a>
 +
                              </li>
 +
                              <li><a href="https://2019.igem.org/Team:Queens_Canada/Collaborations">Team
 +
                                  Quens-Canada</a>
 +
                              </li>
 +
                            </ul>
 +
                          </div>
 +
                   
 +
                      </div>
 +
                    </div>
 
                   </div>
 
                   </div>
 
                 </div>
 
                 </div>
              </div>
+
                <div class="wrap-collabsible"
              <!--hier interlab hin-->
+
                  style="margin-bottom: 25px;">
              <div class="wrap-collabsible">
+
                  <input id="collapsible2_2"
                    <input id="collapsible2_3"
+
                    class="toggle"
                      class="toggle"
+
                    type="checkbox">
                      type="checkbox">
+
                  <label for="collapsible2_2"
                    <label for="collapsible2_3"
+
                    class="lbl-toggle">
                      class="lbl-toggle">
+
                    Slack Channel and gDrive - Global networking in the Golden Gate community
                      <h3 class="title">Slack Channel and gDrive - Global networking in the Golden Gate community</h3>
+
                    <hr style="width: unset;">
                    </label>
+
                  </label>
                    <div class="collapsible-content">
+
                  <div class="collapsible-content">
                      <div class="content-inner"
+
                    <div class="content-inner"
                        style="text-align: left; ">
+
                      style="text-align: left; text-align-last: left;">
                        <div><p>
+
 
                                <h4>Interlab - Accelerate Golden Gate Assembly</h4>
+
 
                                <p>
+
  <div style="display: flex; flex-direction: row;">
                                    <figure Style="float:left; margin-left: 25px;">
+
 
                                        <img style="height: 50ex; width: 40ex" src=" https://static.igem.org/mediawiki/2019/7/79/T--marburg--interlab_button.svg"
+
                        <figure style="text-align: center; margin-right: 25px; min-width:200px">
                                            alt="Interlab button">
+
                          <img src="https://static.igem.org/mediawiki/2019/3/30/T--Marburg--GoldenGateCommunicationButton.svg"
                                    </figure>
+
                            alt="GoldenGateCommunicationButton">
                                    <br> <br>
+
                        </figure>
                                    Interlab - Accelerate Golden Gate Assembly
+
 
                                    As we are constantly working on improving the efficiency of Golden Gate. We clearly
+
                      <p style="margin-bottom: 25px;">
                                    saw the main bottleneck of the reaction was the long assembly time in the cycler.
+
                        Although we were quite pleased with the result of our webinar, we thought about how to
                                    In the webinar and in ourGolden Gate community weprovided protocols with three different cycler settings for different applications.
+
                        further connect this Golden Gate community, so we created a Slack workspace for everyone
                                    Although these threeprotocols were tested before, they were never compared to each other in their
+
                        that is working with this method, enabling us all to exchange protocols and ideas or
                                    efficiency.<br>
+
                        troubleshoot issues that might arise. So far there are close to 50 members, but we hope
                                    We conducted our own Interlab study in collaboration with the iGEM Teams from
+
                        to find more enthusiasts willing to share their knowledge!<br>
                                    <a href="https://2019.igem.org/Team:TUDelft"> TUDelft </a>,
+
</p>
                                    <a href="https://2019.igem.org/Team:Humboldt_Berlin"> HUmboldt Berlin </a> and
+
</div>
                                    <a href="https://2019.igem.org/Team:TU_Kaiserslautern"> TU Kaiserslautern </a>
+
</div>
                                    In order to find the best protocol, we supplied the teams with specific Level 0
+
                     
                                    parts,that should be assembled to a Level 1 vector using the three different cycler conditions.
+
<p style="margin-bottom: 25px;">  
                                    The provided parts areshown in this table:
+
All files used for the webinar are openly available. You can find our protocols,
                                    <br> <br> <!-- Tabelle Links noch rein -->
+
                        handouts, slides as well as the files we used for the practical part in Geneious to
                                    <table>
+
                        construct parts in-silico in our shared Google Drive. Through this we hope to make it
                                            <tbody>
+
                        easier for other teams willing to start with Golden Gate cloning, as they can start
                                                <tr>
+
                        designing their first own in-silico parts with our guidance.
                                                    <td style="text-align: center">5' Connector </td>
+
                      </p>
                                                    <td style="text-align: center"> <a href="link 1"> BBa_K2560075 </a>/
+
                      <p style="margin-bottom: 25px;">
                                                </tr>
+
                        The main documents of the Golgen Gate Webinar<br>
                                                <tr>
+
                        → Download: <a
                                                        <td style="text-align: center">Promoter </td>
+
                          href="https://static.igem.org/mediawiki/2019/0/0a/T--marburg--GG_webinar_slides.pdf">Golden
                                                        <td style="text-align: center"> <a href="link 2">  J23119 BBa_K2560031 </a>
+
                          Gate webinar slide 2019</a><br>
                                                </tr>
+
                        → Download: <a
                                                <tr>
+
                          href="https://static.igem.org/mediawiki/2019/0/09/T--CMUQ--goldenGateWebinarHandout.pdf">Handout
                                                        <td style="text-align: center">RBS </td>
+
                          Golden Gate webinar</a><br>
                                                        <td style="text-align: center"> <a href="link 2">  B0034 BBa_K2560008 </a>
+
                        → Download: <a
                                                </tr>
+
                          href="https://static.igem.org/mediawiki/2019/c/c2/T--marburg--GG_protocoll_white.pdf">Golden
                                                <tr>
+
                          Gate protocoll</a>
                                                        <td style="text-align: center">CDS </td>
+
                      </p>
                                                        <td style="text-align: center"> <a href="link 3"> sfGFP BBa_K2560042  </a>
+
                      <p>
                                                </tr>
+
                        To try out the described in-silico cloning you can download the needed sequences as
                                                <tr>
+
                        geneious file here.<br>
                                                        <td style="text-align: center">Terminator </td>
+
                        → Download: <a href="https://2019.igem.org/File:T--marburg--Sequences_Webinar.zip">Sequences
                                                        <td style="text-align: center"> <a href="link 4"> B0015 BBa_K2560035  </a>
+
                          Webinar </a>
                                                </tr>
+
                      </p>
                                                <tr>
+
                                                        <td style="text-align: center">3' Connector </td>
+
                                                        <td style="text-align: center"> <a href="link 5">  BBa_K2560080 </a>
+
                                                </tr>
+
                                                <tr>
+
                                                        <td style="text-align: center">ORI: </td>
+
                                                        <td style="text-align: center"> <a href="link 6"> ColE1 BBa_K2560036  </a>
+
                                                </tr>
+
                                                <tr>
+
                                                        <td style="text-align: center">Antibiotic-Resistance-Cassette</td>
+
                                                        <td style="text-align: center"> <a href="link 7">  Kan-RFP BBa_K2560056  </a>
+
                                                </tr>
+
                                               
+
                                            </tbody>
+
                                        </table>
+
                                    <br> <!-- Tabelle -->
+
                                    <br> <br>  <br>
+
                                    The Golden Gate reactions should be pipetted together using the given parts and then
+
                                    betransformed in E. coliand be plated with similar amounts on agar plates. A colony with a correct assembled
+
                                    vector would have afunctioning Transcription Unit which expresses GFP, which can be clearly
+
                                    distinguished from RFP expressing colonies which only carries the antibiotic cassette and false non fluorescent
+
                                    colonies. <br>
+
                                    The ratio of green to non-green colonies was used to evaluate which of the three protocols yielded in the
+
                                    highest transformation efficiency and the highest rate of positive clones.
+
                                    <br> <br>
+
                                    <figure Style="text-align:center">
+
                                        <img style="height: 60ex; width:80ex"
+
                                            src="https://static.igem.org/mediawiki/2019/9/91/T--marburg--interlab_package.jpeg"
+
                                            alt="Interlab oackage">
+
                                        <figcaption>
+
                                            Fig 1: Interlab package.
+
                                        </figcaption>
+
                                    </figure>
+
                                    <br>  
+
                                    <br>
+
                                    The handout with the used protocols can be downloaded <a
+
                                        href=https://static.igem.org/mediawiki/2019/a/a0/T--marburg--Interlab_protocoll.pdf>
+
                                        here </a> </p>
+
                           
+
                      </p></div>
+
                      </div>
+
 
                     </div>
 
                     </div>
 
                   </div>
 
                   </div>
 +
                </div>
 +
                <div class="wrap-collabsible"
 +
                  style="margin-bottom: 25px;">
 +
                  <input id="collapsible2_3"
 +
                    class="toggle"
 +
                    type="checkbox">
 +
                  <label for="collapsible2_3"
 +
                    class="lbl-toggle">
 +
                    Interlab - Accelerate Golden Gate Assembly
 +
                    <hr style="width: unset;">
 +
                  </label>
 +
                  <div class="collapsible-content">
 +
                    <div class="content-inner"
 +
                      style="text-align: left;">
 +
                      <div style="display: flex; flex-direction: row;">
 +
                        <p>
 +
 +
                          As we are constantly working on improving the efficiency of Golden Gate. We clearly
 +
                          saw the main bottleneck of the reaction was the long assembly time in the cycler.
 +
                          In the webinar and in our Golden Gate community we provided protocols with three different
 +
                          cycler settings for different applications.
 +
                          Although these three protocols were tested before, they were never compared to each other in
 +
                          their efficiency.<br>
 +
                          We conducted our own Interlab study in collaboration with the iGEM Teams from
 +
                          <a style="padding: 0;"
 +
                            href="https://2019.igem.org/Team:TUDelft"> TUDelft </a>,
 +
                          <a style="padding: 0;"
 +
                            href="https://2019.igem.org/Team:Humboldt_Berlin"> HUmboldt Berlin </a>
 +
                          and
 +
                          <a style="padding: 0;"
 +
                            href="https://2019.igem.org/Team:TU_Kaiserslautern"> TU Kaiserslautern
 +
                          </a>
 +
                          In order to find the best protocol, we supplied the teams with specific Level 0
 +
                          parts,that should be assembled to a Level 1 vector using the three different cycler
 +
                          conditions. The provided parts are shown in the table
 +
                        </p>
 +
                        <div style="display: flex; flex-direction: column; margin-left: 25px;">
 +
                          <figure style="float:left;">
 +
                            <img src="https://static.igem.org/mediawiki/2019/c/c9/T--Marburg--InterlabButton.svg"
 +
                              alt="Interlab button"
 +
                              style="max-height: 200px;">
 +
                          </figure>
 +
 +
 +
                          <table style="min-width: 400px;">
 +
                            <tbody>
 +
                              <tr>
 +
                                <td style="text-align: center">5' Connector </td>
 +
                                <td style="text-align: center"> <a href="link 1"> BBa_K2560075 </a>/
 +
                              </tr>
 +
                              <tr>
 +
                                <td style="text-align: center">Promoter </td>
 +
                                <td style="text-align: center"> <a href="link 2"> J23119 BBa_K2560031 </a>
 +
                              </tr>
 +
                              <tr>
 +
                                <td style="text-align: center">RBS </td>
 +
                                <td style="text-align: center"> <a href="link 2"> B0034 BBa_K2560008 </a>
 +
                              </tr>
 +
                              <tr>
 +
                                <td style="text-align: center">CDS </td>
 +
                                <td style="text-align: center"> <a href="link 3"> sfGFP BBa_K2560042 </a>
 +
                              </tr>
 +
                              <tr>
 +
                                <td style="text-align: center">Terminator </td>
 +
                                <td style="text-align: center"> <a href="link 4"> B0015 BBa_K2560035 </a>
 +
                              </tr>
 +
                              <tr>
 +
                                <td style="text-align: center">3' Connector </td>
 +
                                <td style="text-align: center"> <a href="link 5"> BBa_K2560080 </a>
 +
                              </tr>
 +
                              <tr>
 +
                                <td style="text-align: center">ORI: </td>
 +
                                <td style="text-align: center"> <a href="link 6"> ColE1 BBa_K2560036 </a>
 +
                              </tr>
 +
                              <tr>
 +
                                <td style="text-align: center">Antibiotic-Resistance-Cassette</td>
 +
                                <td style="text-align: center"> <a href="link 7"> Kan-RFP BBa_K2560056 </a>
 +
                              </tr>
 +
                            </tbody>
 +
                          </table>
 +
                        </div>
 +
                      </div>
 +
                      <div style="display: flex; flex-direction: row;">
 +
                        <figure style="text-align: center; margin-right: 25px">
 +
                          <img src="https://static.igem.org/mediawiki/2019/9/91/T--marburg--interlab_package.jpeg"
 +
                            alt="Content of the Interlab package">
 +
                          <figcaption>
 +
                            Fig 1: Content of the Interlab package.
 +
                          </figcaption>
 +
                        </figure>
 +
                        <p>
 +
                          The Golden Gate reactions should be pipetted together using the given parts and then
 +
                          be transformed in <i>E. coli</i> and be plated with similar amounts on agar plates. A colony with a
 +
                          correct assembled
 +
                          vector would have a functioning transcription unit which expresses GFP, which can be clearly
 +
                          distinguished from RFP expressing colonies which only carries the antibiotic cassette and
 +
                          false non fluorescent
 +
                          colonies.<br>
 +
                          The ratio of green to non-green colonies was used to evaluate which of the three protocols
 +
                          yielded in the
 +
                          highest transformation efficiency and the highest rate of positive clones.
 +
                        </p>
 +
                      </div>
 +
                      <p>
 +
                        The handout with the used protocols can be downloaded <a
 +
                          href=https://static.igem.org/mediawiki/2019/a/a0/T--marburg--Interlab_protocoll.pdf>here
 +
                          </a>.
 +
                          </p>
 +
                          <p>
 +
                          <br>
 +
                          <b>Results</b>
 +
                          <br>
 +
 +
<div style="display: flex; flex-direction: row;">
 +
 +
                          <p> First we wanted to know which protocol worked best for every team. For this we analysed the data of the correct assembled green colonies for every protocol. The data of the different teams are given in Table 1 and are shown in as chart in Figure 2. The total colony count is hard to compare directly, cause the numbers differ very much between the teams. This could come through the different transformation efficiencies for example because of the variating competent cells the teams used. </p>
 +
 +
                           
 +
 +
                          <figure style="text-align: center; margin-left: 25px; min-width:500px">
 +
                            <figcaption style="max-width: 500px; text-align: center">
 +
                            Table 1 - Data for the gained green colonies gained by using the three different protocols, from each participating team.
 +
                            </figcaption>
 +
<img
 +
                              src="https://static.igem.org/mediawiki/2019/4/4a/T--Marburg--Tabelle_Interlab.png"
 +
                              alt="Table 1 - Data for the gained green colonies gained by using the three different protocols, from each participating team">
 +
                          </figure>
 +
                          <br>
 +
</div>
 
                      
 
                      
 +
                        <figure style="text-align:center">
 +
                            <img style="height: 400px; width: 500px;"
 +
                              src="https://static.igem.org/mediawiki/2019/3/3a/T--Marburg--Interlab_1.png"
 +
                              alt="Amount of gained green colonies gained by using the three different protocols, from each participating team.">
 +
                            <figcaption style="max-width: 2400px; text-align: center">
 +
                              Fig.2  Amount of gained green colonies gained by using the three different protocols, from each participating team
 +
                            </figcaption>
 +
                          </figure>
 +
 +
  <p>
 +
    <br>
 +
                          The results in Figure 2 show, that the protocols work differently for each team. In general the most colonies were
 +
                          received with the long Troubleshoot protocol, but also the Rapid and Improved protocols show high numbers of correct colonies.
 +
                          <br>
 +
                          But which protocol is the most efficient one?
 +
                          To compare the results of the teams the ratio of the gained correct colonies between the
 +
                          protocols were averaged. The data are shown in Figure 3 and show, that the long Troubleshoot
 +
                          assembly works the best. The fastest assembly (Rapid) works the second best. This is very
 +
                          interesting, because this assembly takes significant less time.<br>
 +
                          <br>
 +
                          We would propose to take the Troubleshoot protocol for complicated assemblies and the rapid
 +
                          protocol for easy assemblies like lvl 0.<br>
 +
</p>
 +
                          <figure style="text-align:center">
 +
                            <img style="height: 400px; width: 500px;"
 +
                              src="https://static.igem.org/mediawiki/2019/a/a1/T--Marburg--Interlab_3.png"
 +
                              alt="Fig.3 Average efficiency of the three protocols">
 +
                            <figcaption style="max-width: 2400px; text-align: center">
 +
                              Fig.3 Average efficiency of the three protocols.
 +
                            </figcaption>
 +
                          </figure>
 +
 +
 +
                      </p>
 +
                    </div>
 
                   </div>
 
                   </div>
 +
                </div>
 +
              </section>
 
             </div>
 
             </div>
 
           </div>
 
           </div>
         </div>  <!--popup3-->
+
         </div>
 
         <div class="sub"
 
         <div class="sub"
           onclick="popup('rbn3')">
+
           onclick="popup('berlin')">
 
           <div class="sub-header">
 
           <div class="sub-header">
             <h1>
+
             <h1>B E R L I N</h1>
                    Berlin/Delft
+
            </h1>
+
 
             <hr>
 
             <hr>
 
           </div>
 
           </div>
 
           <div class="sub-content">
 
           <div class="sub-content">
             <p>
+
             <img src="https://static.igem.org/mediawiki/2019/9/9b/T--marburg--berlin_logo_richtig.png">
             
+
            </p>
+
 
           </div>
 
           </div>
 +
 
         </div>
 
         </div>
         <div id="rbn3"
+
         <div id="berlin"
 
           class="popup">
 
           class="popup">
 
           <div class="popup-container">
 
           <div class="popup-container">
 
             <div class="popup-header">
 
             <div class="popup-header">
               <h1 class="title">Berlin/Delft Collaborations</h1>
+
               <h1 class="title">Berlin Collaboration</h1>
 
               <button type="button"
 
               <button type="button"
                 onclick="hide('rbn3')">X</button>
+
                 onclick="hide('berlin')">X</button>
 
             </div>
 
             </div>
 
             <div class="popup-content"
 
             <div class="popup-content"
 
               style="text-align: justify;">
 
               style="text-align: justify;">
               <div><p>
+
               <div style="display: flex; flex-direction: row;">
                    <figure Style="float:right; margin-left: 25px;">
+
                <p>
                            <img style="height: 15ex; width: 35ex" src="  https://static.igem.org/mediawiki/2019/9/9b/T--marburg--berlin_logo_richtig.png"
+
                  iGEM Berlin engineered the freshwater algae <i>Chlamydomonas reinhardtii</i> to degrade
                                alt="Berlin logo">
+
                  Polyethylene
                        </figure>
+
                  terephthalate
                    iGEM Berlin engineered the freshwater algae Chlamydomonas reinhardtii to degrade Polyethylene
+
                  (PET). For this goal they also built a bioreactor for their chassis. We sent
                    terephthalate
+
                  them our UTEX
                    (PET). For this goal they also built a bioreactor for their chassis. We sent them our UTEX
+
                  strain to test if
                    strain to test if
+
                  their photobioreactor is capable of cultivating other phototrophic organisms as
                    their photobioreactor is capable to cultivate other phototrophic organisms as well. They gained
+
                  well. They gained
                    additional
+
                  additional
                    experiences on growth conditions for phototrophic organisms and we could see our strains grow in
+
                  experiences on growth conditions for phototrophic organisms and we could see our
                    a
+
                  strains grow in
                    bioreactor environment.
+
                  a bioreactor environment.<br>
                    <br>
+
                  Click here to learn more about there project <a
                    Click here to learn more about there project <a
+
                    href="https://2019.igem.org/Team:Humboldt_Berlin">Chlamylicious</a>
                        href="https://2019.igem.org/Team:Humboldt_Berlin"> Chlamylicious </a>
+
                </p>
                    <br>
+
                <figure style="float:right; margin-left: 25px;">
                    <br>
+
                  <img src="https://static.igem.org/mediawiki/2019/9/9b/T--marburg--berlin_logo_richtig.png"
                  <br> <br> <br>
+
                    alt="Berlin logo"
                    <h> <b>Delft Collaborations </b></h> <!-- Delf-->
+
                    style="min-width: 500px;">
                 
+
                </figure>
                 
+
              </div>
                  <figure Style="float:left; margin-right: 25px;" >
+
            </div>
                        <img style="height: 60 ex; width: 40ex"src="https://static.igem.org/mediawiki/2019/5/5e/T--marburg--Skype_DELF.jpeg" alt=" DELFT Bild">
+
          </div>
                    <figcaption> Fig. 1: Skype talk with TU Delft</figcaption>
+
        </div>
                    </figure>
+
        <div class="sub"
                     
+
          onclick="popup('delft')">
                    <figure Style="float:right; margin-left: 25px;">
+
          <div class="sub-header">
                            <img style="height: 30ex; width: 30ex" src="https://static.igem.org/mediawiki/2019/d/d4/T--marburg--DELF_logo_richtig.svg"
+
            <h1>D E L F T</h1>
                                alt="DELF logo">
+
            <hr>
                           
+
          </div>
                        </figure>
+
          <div class="sub-content">
                     <br>
+
            <img src="https://static.igem.org/mediawiki/2019/d/d4/T--marburg--DELF_logo_richtig.svg">
                     iGEM Delft created an all species encompassing toolbox, based on orthogonal gene expression and
+
          </div>
                    host
+
        </div>
                    independent plasmid replication. We exchanged multiple times over Molecular Cloning and toolbox
+
        <div id="delft"
                    designs.
+
          class="popup">
                    Additionally, we sent them parts in the PhytoBricks standard.
+
          <div class="popup-container">
 
+
            <div class="popup-header">
                    Click here to learn more about there project <a href="https://2019.igem.org/Team:TUDelft">
+
              <h1 class="title">Delft Collaboration</h1>
 +
              <button type="button"
 +
                onclick="hide('delft')">X</button>
 +
            </div>
 +
            <div class="popup-content"
 +
              style="text-align: justify;">
 +
              <div style="display: flex; flex-direction: row; margin-top: 25px;">
 +
                <figure style="margin-right: 25px;">
 +
                  <img src="https://static.igem.org/mediawiki/2019/5/5e/T--marburg--Skype_DELF.jpeg"
 +
                    alt=" DELFT Bild"
 +
                    style="max-width: 300px;">
 +
                  <figcaption> Fig. 1: Skype talk with TU Delft</figcaption>
 +
                </figure>
 +
                <div style="display: flex; flex-direction: column;">
 +
                  <div>
 +
                     <h2 class="subtitle">Delft Collaborations</h2>
 +
                     <p>
 +
                      iGEM Delft created an all species encompassing toolbox, based on orthogonal gene
 +
                      expression and
 +
                      host
 +
                      independent plasmid replication. We exchanged multiple times over Molecular
 +
                      Cloning and toolbox
 +
                      designs.
 +
                      Additionally, we sent them parts in the PhytoBricks standard.<br>
 +
                      Click here to learn more about there project <a href="https://2019.igem.org/Team:TUDelft">
 
                         project SCI-PHI 29 </a>
 
                         project SCI-PHI 29 </a>
                 
+
                     </p>
                     <br>
+
                  </div>
              </p></div>
+
                  <figure>
              <br>
+
                    <img src="https://static.igem.org/mediawiki/2019/d/d4/T--marburg--DELF_logo_richtig.svg"
              <br>
+
                      alt="DELF logo"
               <br>
+
                      style="max-height: 200px; margin-top: 25px;">
 +
                  </figure>
 +
                </div>
 +
               </div>
 
             </div>
 
             </div>
 
           </div>
 
           </div>
 
         </div>
 
         </div>
 
         <div class="sub"
 
         <div class="sub"
           onclick="popup('rbn4')"> <!--UC Davis pop up-->
+
           onclick="popup('uc_davis')">
 
           <div class="sub-header">
 
           <div class="sub-header">
 
             <h1>
 
             <h1>
               UC DAVIS
+
               U C &ensp; D A V I S
 
             </h1>
 
             </h1>
 
             <hr>
 
             <hr>
 
           </div>
 
           </div>
           <div class="sub-content">
+
           <div class="sub-content"
             <p>
+
             style="justify-content: center;">
           
+
             <img style="max-height: 100%;"
             </p>
+
              src="https://static.igem.org/mediawiki/2019/d/d7/T--UC_Davis--Teamlogolight.png">
 
           </div>
 
           </div>
 
         </div>
 
         </div>
         <div id="rbn4"
+
         <div id="uc_davis"
 
           class="popup">
 
           class="popup">
 
           <div class="popup-container">
 
           <div class="popup-container">
Line 604: Line 743:
 
               <h1 class="title">UC Davis collaboration</h1>
 
               <h1 class="title">UC Davis collaboration</h1>
 
               <button type="button"
 
               <button type="button"
                 onclick="hide('rbn4')">X</button>
+
                 onclick="hide('uc_davis')">X</button>
 
             </div>
 
             </div>
 
             <div class="popup-content">
 
             <div class="popup-content">
            <div> <p>
+
              <div style="display: flex; flex-direction: row;">
                    <figure Style="float:left; margin-right: 25px;">
+
         
                            <img style="height: 50ex; width: 40ex" src="https://static.igem.org/mediawiki/2019/4/49/T--marburg--UCD_OT2.jpeg"
+
                <div>
                                alt="UCD OT2">
+
                  <p style="margin-bottom: 25px;">
                        </figure>
+
                    UC Davis iGEM team is one of the winners of the Opentrons competition in 2019.
                UC Davis iGEM team is one of the winners of the Opentrons competition in 2019. iGEM Marburg
+
                    iGEM Marburg
                won one OT-2
+
                    won one OT-2
                during last
+
                    during last
                year’s edition of the competition and got contacted for a collaboration by iGEM UC Davis
+
                    year’s edition of the competition and got contacted for a collaboration by iGEM
                along with other
+
                    UC Davis
                winners from
+
                    along with other
                2018 and 2019.
+
                    winners from
                The goal for this automation InterLab collaboration was to collect data to determine the
+
                    2018 and 2019.
                accuracy and
+
                    The goal for this automation InterLab collaboration was to collect data to
                reproducibility of
+
                    determine the
                the OT-2 compared to humans. To do this, UC Davis proposed to perform the standard iGEM
+
                    accuracy and
                protocol on serial
+
                    reproducibility of
                dilutions
+
                    the OT-2 compared to humans. To do this, UC Davis proposed to perform the
                with silica beads and fluorescein, each manually and with an OT-2. In total iGEM Marburg did
+
                    standard iGEM
                four dilutions;
+
                    protocol on serial
                twice with
+
                    dilutions
                an OT-2 and twice manually. The data was then sent to UC Davis via the spreadsheet designed
+
                    with silica beads and fluorescein, each manually and with an OT-2. In total iGEM
                by them.
+
                    Marburg did
                Finally, iGEM
+
                    four dilutions;
                Marburg aided by creating a Slack channel for all iGEM teams with an OT-2 to facilitate
+
                    twice with
                better communication
+
                    an OT-2 and twice manually. The data was then sent to UC Davis via the
                and
+
                    spreadsheet designed
                exchange in the future.  
+
                    by them.
                <br><br>
+
                    Finally, iGEM
                In total, seven teams participated and sent their data to UC Davis which are shown in the
+
                    Marburg aided by creating a Slack channel for all iGEM teams with an OT-2 to
                figure below.
+
                    facilitate
                According to UC
+
                    better communication
                Davis, the fluorescence data was standardized using the iGEM MEFL conversion to standardize
+
                    and
                the plate reader
+
                    exchange in the future.
                measurements. Subsequently, the data was fitted using a linear regression model, whose
+
                  </p>
                quality is quantified
+
<center><figure Style="text-align:center">
                using the
+
                        <!-- add this for centered pics-->
                r2 metric (it ranges from 0 to 1 with higher value indicates a better fit).
+
                        <img src="https://static.igem.org/mediawiki/2019/4/49/T--marburg--UCD_OT2.jpeg" width="450" height="300"
                <br>
+
                    alt="UCD OT2">
                For the fluorescence the r2 value from the OT2 data beats human r2 value with 0.94 and 0.90,
+
                        <figcaption>
                respectively.
+
                            Figure 1: OT-2 setup for the UC Davis collaboration
                Interestingly, for the silica beads, manual measurements yielded a more satisfactory result
+
                        </figcaption>
                with r2 value of
+
                        </figure></center>
                0.90
+
                  <p>
                compared to 0.82 from OT2 measurements. One possible explanation could be the speed of the
+
                    In total, seven teams participated and sent their data to UC Davis which are
                OT-2: it took
+
                    shown in the
                twice as long
+
                    figure below.
                to complete this task with the OT-2 compared to a manual trial. This could lead to the
+
                    According to UC
                sedimentation of the
+
                    Davis, the fluorescence data was standardized using the iGEM MEFL conversion to
                silica beads
+
                    standardize
                as the OT-2 went through their runs; therefore worsening its results. Also, as shown in Fig.
+
                    the plate reader
                2b, one team’s
+
                    measurements. Subsequently, the data was fitted using a linear regression model,
                result
+
                    whose
                deviates a lot from the rest of the teams, maybe if their result is taken from the overall
+
                    quality is quantified
                evaluation a more
+
                    using the
                comparable
+
                    r2 metric (it ranges from 0 to 1 with higher value indicates a better fit).
                result between OT-2 and human can be observed.
+
                    <br><br>
                 <br>
+
                    For the fluorescence the r2 value from the OT2 data beat human r2 value with
                 <br>
+
                    0.94 and 0.90,
                 <figure Style="text-align:center">
+
                    respectively.
                    <img style="height: 50ex; width: 50ex"src="https://static.igem.org/mediawiki/2019/c/cc/T--marburg--UC_Davis_Graphic_1.png"
+
                    Interestingly, for the silica beads, manual measurements yielded a more
                        alt=" UCD Graphic 1">
+
                    satisfactory result
                    <img style="height: 50ex; width: 50ex"src=" https://static.igem.org/mediawiki/2019/a/a7/T--marburg--UC_Davis_Graphic_2.png"
+
                    with r2 value of
                        alt=" UC Davis Graphic 2">
+
                    0.90
                    <figcaption1>
+
                    compared to 0.82 from OT2 measurements. One possible explanation could be the
                        <br> Figure 1: a)OT-2 vs manual fluorescein measurement experiment. OT-2 performed better
+
                    speed of the
                        with r2 value of 0.94 compared to 0.90 of human. b)OT-2 vs manual silica beads measurement experiment. Human performed better
+
                    OT-2: it took
                        with r2 value of 0.90 compared to 0.82 of OT-2.
+
                    twice as long
                    </figcaption>    
+
                    to complete this task with the OT-2 compared to a manual trial. This could lead
                </figure>
+
                    to the
                <br>
+
                    sedimentation of the
            </p>
+
                    silica beads
            <br>More information about this collaboration such as individual team data can be found on UC
+
                    as the OT-2 went through their runs; therefore worsening its results. Also, as
            Davis wiki:
+
                    shown in Fig.
            <a href="https://2019.igem.org/Team:UC_Davis/Collaborations"> Wiki UC Davis Collaboration </a>
+
                    2b, one team’s
            <br>
+
                    result
              </p></div>
+
                    deviates a lot from the rest of the teams, maybe if their result is taken out from
           
+
                    the overall
              <br>
+
                    evaluation a more
       
+
                    comparable
 +
                    result between OT-2 and human could be observed.
 +
                  </p>
 +
                 </div>
 +
              </div>
 +
              <p style="margin-top: 25px;">
 +
                 More information about this collaboration such as individual team data can be
 +
                found on <a href="https://2019.igem.org/Team:UC_Davis/Collaborations"> UC Davis wiki.
 +
                  </a>
 +
                  
 +
              </p>
 +
              <figure style="text-align: center; margin-top: 25px;">
 +
                <img style="max-height: 450px"
 +
                  src="https://static.igem.org/mediawiki/2019/c/cc/T--marburg--UC_Davis_Graphic_1.png"
 +
                  alt=" UCD Graphic 1">
 +
                <img style="max-height: 450px"
 +
                  src=" https://static.igem.org/mediawiki/2019/a/a7/T--marburg--UC_Davis_Graphic_2.png"
 +
                  alt=" UC Davis Graphic 2">
 +
                <figcaption>
 +
                  Figure 2: a) OT-2 vs manual fluorescein measurement experiment, OT-2
 +
                  performed better
 +
                  with r2 value of 0.94 compared to 0.90 of human. b) OT-2 vs manual silica
 +
                  beads measurement experiment, human performed better
 +
                  with r2 value of 0.90 compared to 0.82 of OT-2.
 +
                </figcaption>
 +
              </figure>
 
             </div>
 
             </div>
 
           </div>
 
           </div>
 
         </div>
 
         </div>
 
         <div class="sub"
 
         <div class="sub"
           onclick="popup('rbn5')"> <!-- UFlorida-->
+
           onclick="popup('uflorida')">
 
           <div class="sub-header">
 
           <div class="sub-header">
 
             <h1>
 
             <h1>
               UFLORIDA
+
               U F L O R I D A
 
             </h1>
 
             </h1>
 
             <hr>
 
             <hr>
 
           </div>
 
           </div>
 
           <div class="sub-content">
 
           <div class="sub-content">
             <p>
+
             <img src="https://static.igem.org/mediawiki/2019/f/f7/T--Florida--iGEMWeb1.png">
            </p>
+
 
           </div>
 
           </div>
 
         </div>
 
         </div>
         <div id="rbn5"
+
         <div id="uflorida"
 
           class="popup">
 
           class="popup">
 
           <div class="popup-container">
 
           <div class="popup-container">
Line 709: Line 872:
 
               <h1 class="title">UFlorida</h1>
 
               <h1 class="title">UFlorida</h1>
 
               <button type="button"
 
               <button type="button"
                 onclick="hide('rbn5')">X</button>
+
                 onclick="hide('uflorida')">X</button>
 
             </div>
 
             </div>
 
             <div class="popup-content"
 
             <div class="popup-content"
               style="text-align: justify;>
+
               style="text-align: justify">
               <div></div><p>
+
               <div style="display: flex; flex-direction: row;">
                    <br>
+
                <figure style="float:left; margin-right: 25px;">
 +
                  <img src="https://static.igem.org/mediawiki/2019/f/f7/T--Florida--iGEMWeb1.png"
 +
                    alt="Florida">
 +
                </figure>
 +
                <p>
 +
                  This semester we had the pleasure of mentoring UFlorida for their Wiki
 +
                  design. Our member, René Inckemann, aided UFlorida in their quest to create
 +
                  a visually accessible Wiki, similar to what we did this past iGEM season.
 +
                  We helped shape UFlorida’s website into an interactive and appealing
 +
                  landscape, while maintaining the integrity of their goal and project. We
 +
                  advised them on their font choices- specifically their use of bold and
 +
                  italicized words-, along with answering questions about our work with the
 +
                  visually disabled community and utilizing our “Accessible Wiki Guide” from
 +
                  the previous year.
 +
                  <br>
 +
                  Besides answering questions specifically about their project, we also
 +
                  offered valuable information about when to start working on the Wiki and
 +
                  questions about coding. Working with a young team like UFlorida offered us
 +
                  a great mentorship opportunity.
  
                    <figure  Style="float:left; margin-right: 25px;">
+
<a style="padding: 0;"
                            <img style="height: 30ex; width: 40ex" src="https://static.igem.org/mediawiki/2019/f/f7/T--Florida--iGEMWeb1.png" alt="Florida">
+
                        href="https://2019.igem.org/Team:Florida/Collaborations"> Click here to learn more about there project.
                            <figcaption>
+
                      </a>
                           
+
                            </figcaption>
+
                        </figure>
+
                    This semester we had the pleasure of mentoring UFlorida for their Wiki
+
                    design. Our member, Rene Inckemann, aided UFlorida in their quest to create
+
                    a visually accessible Wiki, similar to what we did this past iGEM season.
+
                    We helped shape UFlorida’s website into an interactive and appealing
+
                    landscape, while maintaining the integrity of their goal and project. We
+
                    advised them on their font choices- specifically their use of bold and
+
                    italicized words-, along with answering questions about our work with the
+
                    visually disabled community and utilizing our “Accessible Wiki Guide” from
+
                    the previous year.
+
                    <br>
+
                    Besides answering questions specifically about their project, we also
+
                    offered valuable information about when to start working on the Wiki and
+
                    questions about coding. Working with a young team like UFlorida offered us
+
                    a great mentorship opportunity.
+
                    <br>
+
  
                    <figure style="align-content:center;">
+
 
                        <img src="https://static.igem.org/mediawiki/2019/2/29/T--marburg--UFlorida_Rene.jpeg" alt="Florida">
+
                </p>
                        <figcaption>
+
              </div>
                            Fig. 1: Skypecall with UFlorida
+
              <figure style="align-content:center; margin-top: 25px;">
                        </figcaption>
+
                <img src="https://static.igem.org/mediawiki/2019/2/29/T--marburg--UFlorida_Rene.jpeg"
                    </figure>
+
                  alt="Florida">
                    <br>
+
                <figcaption>
              </p></div>
+
                  Fig. 1: Skypecall with UFlorida
              <br>
+
                </figcaption>
              <br>
+
              </figure>
 
             </div>
 
             </div>
 
           </div>
 
           </div>
          <div class="sub"
+
        </div>
           onclick="popup('rbn6')"> <!-- mini collabs-->
+
        <div class="sub"
 +
           onclick="popup('interaction')">
 
           <div class="sub-header">
 
           <div class="sub-header">
             <h1>
+
             <h1>I N T E R A C T I O N S</h1>
              INTERACTIONS WITH <br>
+
            <h1>W I T H</h1>
              OTHER TEAMS
+
             <h1>O T H E R &ensp; T E A M S</h1>
             </h1>
+
 
             <hr>
 
             <hr>
 
           </div>
 
           </div>
 
           <div class="sub-content">
 
           <div class="sub-content">
             <p>
+
             <img src="https://static.igem.org/mediawiki/2019/f/fe/T--marburg--Postcard.png">
            </p>
+
 
           </div>
 
           </div>
 
         </div>
 
         </div>
         <div id="rbn6"
+
         <div id="interaction"
 
           class="popup">
 
           class="popup">
 
           <div class="popup-container">
 
           <div class="popup-container">
 
             <div class="popup-header">
 
             <div class="popup-header">
               <h1 class="title">UFlorida</h1>
+
               <h1 class="title">Interactions with other teams</h1>
 
               <button type="button"
 
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               <h2 class="subtitle">Mike the microbe</h2>
                   
+
              <div style="display: flex; flex-direction: row;">
                    <b>Mike the microbe</b>  
+
                <figure Style="text-align: center; margin-right: 25px;">
                    <figure Style="text-align:center">
+
                  <img src="https://static.igem.org/mediawiki/2019/b/b0/T--marburg--Mike_microbe.png"
                        <img src="   https://static.igem.org/mediawiki/2019/b/b0/T--marburg--Mike_microbe.png"
+
                    alt=" Mike the Mircrobe"
                            alt=" Mike the Mircrobe">
+
                    style="min-width: 300px;">
                        <figcaption>
+
                  <figcaption>
                            Figure 1: Mike the mircrobe in our lab.
+
                    Figure 1: Mike the mircrobe in our lab.
                        </figcaption>
+
                  </figcaption>
                    </figure>
+
                </figure>
                    We participated in the “Mike the microbe” collaboration of the iGEM Team US AFRL CarrollHS. They
+
                <p>
                    sent us
+
                  We participated in the “Mike the microbe” collaboration of the iGEM Team US AFRL
                    their virtual mascots Mike the Microbe and Chia the Chitinase so we could take pictures with them to
+
                  CarrollHS.
                    connect
+
                  They
                    all iGEM teams around the world via social media. It brought us great joy to join to this
+
                  sent us
                    collaboration and
+
                  their virtual mascots Mike the Microbe and Chia the Chitinase so we could take pictures
                    let the two little buddies visit our daily life in our incubators for cyanobacteria.
+
                  with
                    <br>
+
                  them to
                    <br>
+
                  connect
 +
                  all iGEM teams around the world via social media. It brought us great joy to join to this
 +
                  collaboration and
 +
                  let the two little buddies visit our daily life in our incubators for cyanobacteria.
 
                 </p>
 
                 </p>
                 <br>
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              </div>
 +
              <h2 class="subtitle"
 +
                 style="margin-top: 25px;">Postcards Düsseldorf</h2>
 +
              <div style="display: flex; flex-direction: row;">
 
                 <p>
 
                 <p>
                    <h4><b>Postcards Düsseldorf</b></h4>    <!-- Postcard challenge-->
+
                  iGEM Düsseldorf called for their traditional collaboration project, a long year postcard
                        iGEM Düsseldorf called for their traditional collaboration project, a long year postcard
+
                  campaign with the
                        campaign with the
+
                  purpose to educate and to show the public about the different topics and projects in
                        purpose to educate and to show the public about the different topics and projects in Synthetic
+
                  Synthetic
                        Biology and
+
                  Biology and
                        especially regarding all the iGEM projects. With interesting designs and a short project
+
                  especially regarding all the iGEM projects. With interesting designs and a short project
                        abstract iGEM teams
+
                  abstract iGEM teams
                        could spread their ideas using the postcards to share their thinking to each other and the
+
                  could spread their ideas using the postcards to share their thinking to each other and the
                        society. Our
+
                  society. Our
                        title and postcard design was about our photosynthetic project „Syntex“. You can see
+
                  title and postcard design was about our photosynthetic project „Syntex“. You can see
                        Synechococcus elongatus
+
                  <i>Synechococcus elongatus</i>
                        UTEX 2973 direly needing a refreshing drink, a cute little draft from our team member Jonas.
+
                  UTEX 2973 direly needing a refreshing drink, a cute little draft from our team member
                        <br>
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                  Jonas.
                        <figure Style="text-align:center">
+
                            <img src="    https://static.igem.org/mediawiki/2019/f/fe/T--marburg--Postcard.png"
+
                                alt="Postcard challenge">
+
                            <figcaption>
+
                                Figure 2: Our Postcard for the Postcard collaboration from Düsseldorf.
+
                            </figcaption>
+
                        </figure>
+
                        <br>
+
 
                 </p>
 
                 </p>
                 <br>
+
                 <figure style="text-align:center; margin-left: 25px;">
 +
                  <img src="https://static.igem.org/mediawiki/2019/f/fe/T--marburg--Postcard.png"
 +
                    alt="Postcard challenge"
 +
                    style="min-width: 300px;">
 +
                  <figcaption>
 +
                    Figure 2: Our Postcard for the Postcard collaboration from Düsseldorf.
 +
                  </figcaption>
 +
                </figure>
 +
              </div>
 +
              <h2 class="subtitle"
 +
                style="margin-top: 25px;">iGEMxSDGs challenge</h2>
 +
              <div style="display: flex; flex-direction: row;">
 +
                <figure style="text-align:center; margin-right: 25px;">
 +
                  <img src="https://static.igem.org/mediawiki/2019/1/18/T--marburg--SDG_chalenge.png"
 +
                    alt="SGD Challenge - 7 12 13 15"
 +
                    style="min-width: 300px;">
 +
                  <figcaption>
 +
                    Figure 3: SDG challenge.
 +
                  </figcaption>
 +
                </figure>
 
                 <p>
 
                 <p>
                    <h5><b>iGEMxSDGs challenge</b></h5>
+
                  IGEM Costa Rica, Tuebingen, and TAS Taipei created an iGEMxSDGs challenge as
                    IGEM Costa Rica, Tuebingen, and TAS Taipei created an iGEMxSDGs challenge as collaboration. They
+
                  collaboration.
                    asked us to
+
                  They
                    choose four of the Sustainable Development Goals (SDGs) proposed by the United Nations (UN). We
+
                  asked us to
                    joined this
+
                  choose four of the Sustainable Development Goals (SDGs) proposed by the United Nations
                    collaboration challenge and spread it on our social media account. We decided on the following
+
                  (UN).
                    goals:
+
                  We
                    <br>
+
                  joined this
                    <figure Style="text-align:center">
+
                  collaboration challenge and spread it on our social media account. Our project targets the
                        <img src="  https://static.igem.org/mediawiki/2019/1/18/T--marburg--SDG_chalenge.png"
+
                  goals shown in Figure 3.  
                            alt="SGD Challenge">
+
                        <figcaption>
+
                            Figure 3: SDG challenge.
+
                        </figcaption>
+
                    </figure>
+
                    <br>
+
 
                 </p>
 
                 </p>
                 <br>
+
              </div>
 
+
              <h2 class="subtitle"
 +
                 style="margin-top: 25px;">Who wore it best? #labcoatchallenge</h2>
 +
              <div style="display: flex; flex-direction: row;">
 
                 <p>
 
                 <p>
                    <h5><b>Who wore it best? #labcoatchallenge</b></h5>
+
                  The Stony Brook iGEM Team challenged us to design an outfit for a runway from a standard
                    The Stony Brook iGEM Team challenged us to design an outfit for a runway from a standard lab coat.
+
                  lab
                    The
+
                  coat.
                    outfit had to be created by tying, not using duct tape, cut or sew, etc. After designing our outfits
+
                  The
                    four of
+
                  outfit had to be created by tying, not using duct tape, cut or sew, etc. After designing
                    our members presented their style on our ‘runway’ while paparazzi took their photos. As an outfit we
+
                  our
                    chose a
+
                  outfits
                    dress highlighting the neckline, a shoulder free dress, a knotted dress and a skirt to bring glamour
+
                  four of
                    to the
+
                  our members presented their style on our ‘runway’ while paparazzi took their photos. As an
                    daily routine in the labs. Especially our guys were rocking their outfits as you can see here
+
                  outfit we
                    x
+
                  chose a
                    <br>
+
                  dress highlighting the neckline, a shoulder free dress, a knotted dress and a skirt to
                    <figure Style="text-align:center">
+
                  bring
                        <img style="height: 75ex; width: 60ex" src="https://static.igem.org/mediawiki/2019/7/74/T--marburg--Labcoat_Burgi.jpeg" alt="labcoat">
+
                  glamour
                        <figcaption>
+
                  to the
                            Figure 4: Our model Burgis for the labcoatchallenge.
+
                  daily routine in the labs. Especially our guys were rocking their outfits as you can see
                        </figcaption>
+
                  in Figure 4.
                    </figure>
+
                </p>
                    <br>
+
                <figure style="text-align:center; margin-left: 25px;">
               </p></div>
+
                  <img src="https://static.igem.org/mediawiki/2019/7/74/T--marburg--Labcoat_Burgi.jpeg"
              <br>
+
                    alt="Our model Burgis for the labcoatchallenge"
              <br>
+
                    style="min-width: 300px;">
 +
                  <figcaption>
 +
                    Figure 4: Our model Burgis for the labcoatchallenge.
 +
                  </figcaption>
 +
                </figure>
 +
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             <h1>
+
             <h1>M E E T U P S</h1>
              MEETUPS
+
            </h1>
+
 
             <hr>
 
             <hr>
 
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            </p>
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           </div>
 
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Line 883: Line 1,061:
 
               <h1 class="title">Meet ups</h1>
 
               <h1 class="title">Meet ups</h1>
 
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               <h2 class="subtitle">iGEM Spring Festival in Bonn</h2>
                   
+
              <div style="display: flex; flex-direction: row;">
                    <h2><b>iGEM Spring Festival in Bonn</b></h2>
+
                <p>
                    From 3rd 5th Mai we met our iGEM colleagues for the Spring Festival in Bonn. Professors of the university of Bonn presented their research fields which gained huge interest. We heard lectures of immunology and neurosciences, as well as reviving mammoths. During the lectures, specific topics were deepened in small seminar groups, such as the ethical tenability of CRISPR applied at humans and other animals. At the poster sessions we got the first time in contact with the other teams’ projects. Afterwards we had the opportunity to vote for the best project, which iGEM Düsseldorf won. Besides that, the meetup was filled with a lot of awarding categories. We competed in the categories for “Best Video”, “Best Poster”, “Best Project” and a single person had the chance to win the award for “Best Meme”. The iGEM Team Bonn offered spontaneously a little football table tournament, where two of our team members won. In the evening we learnt a lot about Bonn as a city from a guide living there for ages. Afterwards, the iGEM Teams met in several groups in restaurants and bars. We merged with iGEM Kaiserslautern and exchanged our projects and ideas for the rest of the evening.
+
                  From the 3rd to the 5th Mai we met other iGEMers at the Spring Festival in Bonn. Professors
                    <br>
+
                  of the university of Bonn presented their research fields which was met with huge interest. We heard
                    On the second day, the iGEM Team Bonn offered a competition out of three rounds, where we competed against other teams. We were asked iGEM specific questions, had to solve riddles for an escape room and finished a lab challenge afterwards. Our team could compete until the end and won the prize! Thanks again to Doulix for this awesome prize!  
+
                  lectures
                    <br>
+
                  about immunology and neurosciences, as well as reviving mammoths. During the lectures,
                    We particularly thank the iGEM Team Bonn for the huge effort and the great meetup!
+
                  specific
                    <br>
+
                  topics were deepened in small seminar groups, such as the ethical tenability of CRISPR
                    <figure Style="text-align:center">
+
                  applied
                            <img style="height: 50ex; width: 65ex" src="https://static.igem.org/mediawiki/2019/a/a9/T--marburg--meet_u_bonn.png" alt="Erlangen meetup">
+
                  at humans and other animals. At the poster sessions we got the first time in contact with
                            <figcaption>
+
                  other teams’ projects. Afterwards, we had the opportunity to vote for the best project,
                                    Fig. 1: Two representatives of Doulix celebrating with us.
+
                  which
                            </figcaption>
+
                  iGEM Düsseldorf won. Besides that, the meetup was filled with a lot of awarding
                    </figure>
+
                  categories.
               
+
                  We
                 <br>
+
                  competed in the categories for “Best Video”, “Best Poster”, “Best Project” and a single
                   
+
                  person
                            <h3><b>Erlangen collaboration meetup</b></h3>
+
                  had the chance to win the award for “Best Meme”. The iGEM Team Bonn offered spontaneously
                            On the 17th 19th of May we attended the collaboration meetup hosted by the iGEM Team Erlangen 2019. There, we met the iGEM teams of Straßbourg, Tübingen, Vienna and of course Erlangen. Apart from listening to the interesting talks from the various professors there, we made a lot of new friends and took the chance to talk about possible collaborations with the other teams and also promote our collaborations, such as the colony picking project and the Golden Gate webinar (evtl. verlinken). We would like to thank iGEM Team Erlangen very much for organizing this wonderful meetup and are happy to see them soon in Boston.
+
                  a
                            <br>
+
                  little football table tournament, where two of our team members won. In the evening we
                           
+
                  learnt a
                            <figure Style="text-align:center">
+
                  lot about Bonn as a city from a guide living there for many years. Subsequently, the iGEM Teams
                                    <img style="height: 40ex; width: 65ex" src="https://static.igem.org/mediawiki/2019/0/01/T--marburg--meet_up_erlangen.jpeg" alt="Erlangen meetup">
+
                  met
                                    <figcaption>
+
                  in
                                            Fig. 2: Group photo from the Erlangen mett up (Michael Burgis from Team Marburg in the left side).
+
                  several groups in restaurants and bars. We merged with iGEM Kaiserslautern and exchanged
                                    </figcaption>
+
                  our
                                </figure>
+
                  projects and ideas for the rest of the evening.<br>
                   
+
                  On the second day, the iGEM Team Bonn offered a competition out of three rounds, where we
                    <br>
+
                  competed against other teams. We were asked iGEM specific questions, had to solve riddles
                     
+
                  for an
                            <h><b>Düsseldorf meetup</b></h>
+
                  escape room and finished a lab challenge afterwards. Our team could compete until the end
                            <br>From 5th 7th July we went to the HHU for the German meetup. The calendar offered lots of lectures with interesting topics such as the Excellent Cluster of Prof. Andreas Weber “CEPLAS” but also non-scientific aspects like how to do presentations. We tried to visit all of them while presenting our poster in the hall. There we got in touch with other iGEM Teams also learnt about their projects. We promoted our Webinar project and laid the foundation stone for future collaborations. On the second day, iGEM Düsseldorf organized a panel discussion about the issues concerning green genetic engineering. Thereby, we got great input on how to structure our panel discussion and which subjects should be mentioned. Beside the regular program organized by iGEM Düsseldorf we used a lot of chances to get in contact with other teams. After the presentations a couple of tourist guides showed us the beautiful old town of Düsseldorf and explained some interesting historical facts. Afterwards we had again the possibility to meet with other iGEMers in a pub and talk about the projects, especially to find weaknesses or opportunities for collaborations. This really went successfully due to the time we spend with iGEM Team Aachen and Kaiserslautern. The iGEM Team Düsseldorf also arranged a game contest between the teams, where we lost in the final to our good friends from Potsdam. Congrats! <br>
+
                  and
                           
+
                  won the prize! Thanks again to Doulix for this awesome prize! <br>
                            <figure Style="text-align:center">
+
                  We particularly thank the iGEM Team Bonn for the huge effort and the great meetup!
                                    <img style="height: 70ex; width: 50ex" src="https://static.igem.org/mediawiki/2019/e/e4/T--marburg--meet_up_Duesseldorf.jpeg" alt="Du meetup">
+
                </p>
                                    <figcaption>
+
                <figure style="text-align:center; margin-left: 25px">
                                            Fig. 3: Team photo from the Düsseldorf meetup.
+
                  <img style="min-width: 300px;"
                                    </figcaption>  
+
                    src="https://static.igem.org/mediawiki/2019/a/a9/T--marburg--meet_u_bonn.png"
                                      </figure>        
+
                    alt="Erlangen meetup">
                                    <br>Thanks again to iGEM Düsseldorf for the huge effort and the great meetup! <br>
+
                  <figcaption>
                                  </p>
+
                    Fig. 1: Two representatives of Doulix celebrating with us.
                  <br>
+
                  </figcaption>
                       
+
                </figure>
                            <h><b> Vilnius, Lithuania – Biohackathon Lab App 2019</b></h>
+
              </div>
                            <br>In August 2019 our Team participated in the Biohackathon Lab App, organized by the Vilnius-Lithuania Team 2019. Together with the iGEM Teams Stockholm and UCopenhagen, as well as several non-iGEM Teams we sat together for a weekend packed with problems, ideas and neat solutions.
+
              <h2 class="subtitle"
                            With the task of creating an application that tackles a self-found problem, we sat together and brainstormed about what could be done.
+
                 style="margin-top: 25px;">Erlangen collaboration meetup</h2>
                            <br>
+
              <div style="display: flex; flex-direction: row;">
                            During this time we were starting to work more with the Opentrons OT-2 and soon realized that some of our biologists had trouble integrating protocols in the robot themselves, as this is primarily done by writing the protocol in Python and pushing it directly to the robot – no convenient application to change parameters, such as the amount of reactions,was included in the protocols. In our heads the idea to have a user-friendly GUI grew more and more and soon we found ourselves standing in the Vilnius University Life Sciences Center.
+
                <figure style="text-align:center; margin-right: 25px">
                            <br>“We” were two biologists and one mathematician, so we talked to some of the other participants that did not have a team yet and found the perfect fit to complement our small team: Nour Alsamsam was happy to join us for this weekend and provided us with his coding skills – huge thank you to you, Nour!
+
                  <img style="min-width: 300px;"
                            Together we tried our best to come up with a suitable app and presented it in the end, having more ideas in our heads than we could implement in such a short time – it was clear for us, that we would continue with this project later on!
+
                    src="https://static.igem.org/mediawiki/2019/0/01/T--marburg--meet_up_erlangen.jpeg"
                            <br>In conclusion, the Biohackathon was an awesome and productive meeting of the iGEM Teams Stockholm, Copenhagen, Vilnius and Marburg. We talked about our projects, offered each other feedback on current issues in the lab and shared many ideas.
+
                    alt="Erlangen meetup">
                            We are very grateful that we got the opportunity to meet so many experts from different fields, which really helped getting a fresh perspective on our project and are now looking forward to meet the other teams again at the Giant Jamboree!
+
                  <figcaption>
                            <br>
+
                    Fig. 2: Group photo from the Erlangen mett up (Michael Burgis from Team Marburg in the
                            <figure Style="text-align:center">
+
                    left
                                    <img  src="Pic" alt="Vilnius hacker">
+
                    side).
                                    <figcaption>
+
                  </figcaption>
                                            Fig. 4: Meet up Vilnius, Lithuania .
+
                </figure>
                                    </figcaption>
+
                <p>
                                </figure>
+
                  On the 17th till the 19th of May we attended the collaboration meetup hosted by the iGEM team
 +
                  Erlangen
 +
                  2019. There, we met the iGEM teams of Straßbourg, Tübingen, Vienna and of course Erlangen.
 +
                  Apart
 +
                  from listening to the interesting talks from the various professors there, we made a lot
 +
                  of
 +
                  new
 +
                  friends and took the chance to talk about possible collaborations with the other teams and
 +
                  also
 +
                  promote our collaborations, such as the <a
 +
                    href="https://2019.igem.org/Team:Marburg/Collaborations#colony_pictures"
 +
                    target="_blank">colony picking project</a> and the <a
 +
                    href="https://2019.igem.org/Team:Marburg/Collaborations#golden_gate"
 +
                    target="_blank">Golden Gate webinar</a>. We would like to thank iGEM team Erlangen very much for
 +
                  organizing this
 +
                  wonderful meetup!
 +
                </p>
 +
              </div>
 +
              <h2 class="subtitle"
 +
                style="margin-top: 25px;">Düsseldorf meetup</h2>
 +
              <div style="display: flex; flex-direction: row;">
 +
                <p>
 +
                  From the 5th till the 7th July we went to the HHU for the German meetup. Many lectures with interesting topics
 +
                  were offered by the Cluster of Exellence of Plant Science (<a href="https://www.ceplas.eu/en/home/"
 +
                    target="_blank">CEPLAS</a>) by Prof. Andreas Weber
 +
                  but also non-scientific aspects like courses on presentations. We tried to visit all of
 +
                  them
 +
                  while presenting our poster in the hall. There we got in touch with other iGEM Teams and also
 +
                  learnt
 +
                  about their projects. We promoted our Webinar project and laid the foundation stone for
 +
                  future
 +
                  collaborations. On the second day, iGEM Düsseldorf organized a panel discussion about the
 +
                  issues
 +
                  concerning green genetic engineering. Thereby, we got great input on how to structure our
 +
                  panel
 +
                  discussion and which subjects should be mentioned. Beside the regular program organized by
 +
                  iGEM
 +
                  Düsseldorf we used a lot of chances to get in contact with other teams. After the
 +
                  presentations
 +
                  a couple of tourist guides showed us the beautiful old town of Düsseldorf and explained
 +
                  some
 +
                  interesting historical facts. Afterwards we had again the possibility to meet with other
 +
                  iGEMers
 +
                  in a pub and talk about the projects, especially to find weaknesses or opportunities for
 +
                  collaborations. This really went successfully due to the time we spend with iGEM Team Aachen, Kaiserslautern and Hamburg. The iGEM Team Düsseldorf also arranged a game contest between the teams,
 +
                  where
 +
                  we lost in the final to our good friends from Potsdam. Congrats! <br>
 +
                  Thanks again to iGEM Düsseldorf for the huge effort and the great meetup!
 +
                </p>
 +
                <figure style="text-align:center; margin-left: 25px;">
 +
                  <img style="min-width: 200px;"
 +
                    src="https://static.igem.org/mediawiki/2019/e/e4/T--marburg--meet_up_Duesseldorf.jpeg"
 +
                    alt="Du meetup">
 +
                  <figcaption>
 +
                    Fig. 3: Team photo from the Düsseldorf meetup.
 +
                  </figcaption>
 +
                </figure>
 +
              </div>
 +
              <h2 class="subtitle"
 +
                style="margin-top: 25px;">Vilnius, Lithuania – Biohackathon Lab App 2019
 +
              </h2>
 +
              <div style="display: flex; flex-direction: row;">
  
              </p></div>
+
                <figure style="text-align:center; margin-right: 25px">
              <br>
+
                  <img style="min-width: 300px;"
              <br>
+
                    src="https://static.igem.org/mediawiki/2019/4/4e/T--Marburg--vilnius.jpg"
 +
                    alt="Vilnius biohackathon">
 +
                  <figcaption>
 +
                    Fig. 4: Biohackathon in Vilnius.
 +
                  </figcaption>
 +
                </figure>
 +
                <p>
 +
                  In August 2019 our Team participated in the Biohackathon Lab App, organized by the
 +
                  Vilnius-Lithuania Team 2019. Together with the iGEM Teams Stockholm and UCopenhagen, as
 +
                  well
 +
                  as
 +
                  several non-iGEM Teams we sat together for a weekend packed with problems, ideas and neat
 +
                  solutions.
 +
                  With the task of creating an application that tackles a self-found problem, we sat
 +
                  together
 +
                  and
 +
                  brainstormed about what could be done.
 +
                  <br>
 +
                  During this time we were starting to work more with the Opentrons OT-2 and soon realized
 +
                  that
 +
                  some
 +
                  of our biologists had trouble integrating protocols in the robot themselves, as this is
 +
                  primarily
 +
                  done by writing the protocol in Python and pushing it directly to the robot – no
 +
                  convenient
 +
                  application to change parameters, such as the amount of reactions,was included in the
 +
                  protocols.
 +
                  In our heads the idea to have a user-friendly GUI grew more and more and soon we found
 +
                  ourselves
 +
                  standing in the Vilnius University Life Sciences Center.
 +
                  <br>“We” were two biologists and one mathematician, so we talked to some of the other
 +
                  participants
 +
                  that did not have a team yet and found the perfect fit to complement our small team: Nour
 +
                  Alsamsam
 +
                  was happy to join us for this weekend and provided us with his coding skills – huge thank
 +
                  you
 +
                  to
 +
                  you, Nour!
 +
                  Together we tried our best to come up with a suitable app and presented it in the end,
 +
                  having
 +
                  more
 +
                  ideas in our heads than we could implement in such a short time – it was clear for us,
 +
                  that we
 +
                  would continue with this project later on!
 +
                  <br>In conclusion, the Biohackathon was an awesome and productive meeting of the iGEM
 +
                  teams
 +
                  Stockholm, Copenhagen, Vilnius and Marburg. We talked about our projects, offered each
 +
                  other
 +
                  feedback on current issues in the lab and shared many ideas.
 +
                  We are very grateful that we got the opportunity to meet so many experts from different
 +
                  fields,
 +
                  which really helped getting a fresh perspective on our project!
 +
                </p>
 +
              </div>
 
             </div>
 
             </div>
 
           </div>
 
           </div>
 
         </div>
 
         </div>
      </section>
 
 
     </div>
 
     </div>
 +
    </section>
 
   </div>
 
   </div>
  </body>
 
 
 
</html>
 
</html>
 
{{Marburg/footer}}
 
{{Marburg/footer}}

Latest revision as of 06:45, 9 December 2019

C O L L A B O R A T I O N S


Through this iGEM year we interacted with many iGEM Teams all around the world. It was a pleasure working with all the different teams and learn from each other. We got many impressions about other projects and we hope that we could help with our collaborations. We would like to thank every team that collaborated with us!


C O L O N Y
P I C T U R E S


T H E   G O L D E N   G A T E

C O L L A B O R A T I O N


B E R L I N


D E L F T


U C   D A V I S


U F L O R I D A


I N T E R A C T I O N S

W I T H

O T H E R   T E A M S


M E E T U P S