P A R T C O L L E C T I O N
We proudly present the Marburg Collection 2.0, consisting of the Marburg Collection from last year’s iGEM
and the numerous additions that were made this year.
The Marburg Collection 1.0 is a Golden Gate based toolbox containing 123 parts, including promoters, reporters, fluorescence and epitope tags, oris, resistance cassettes and genome engineering tools. A specialty in this toolbox are the 36 connectors with which multigene constructs can be easily and flexibly cloned. They also allow the inversion of individual transcription units and function as insulators to avoid undesired crosstalk.
The Marburg Collection 2.0 adds placeholders to our arsenal, which allow the large scale assembly of plasmids for screenings and characterizations as well as another addition we called the Green Expansion.
This expansion features the world's first MoClo compatible shuttle vector for cyanobacteria (K3228069). This composite part contains the origins of replication ColE1 (for cloning) and the ori of panS (for maintenance in cyanobacteria) as well as a spectinomycin cassette. It can be used in the Marburg Collection like any other composite part of the type 7+8 (antibiotic cassette + origin of replication).
This expansion also contains all the parts needed for the genomic integration of one or multiple genes in cyanobacteria. It convinces with a striking flexibility and a very intuitive workflow for the de novo assembly of your plasmid of choice. It encompasses five different neutral integration sites to choose from: three conventional sites frequently used in the cyanobacterial community (NSI to NSIII) as well as our own rationally designed artificial neutral integration site options (a.N.S.o. 1 and 2). These sites show no transcriptional activity from neighboring regions and are therefore completely orthogonal. Additionally we offer 4 different antibiotic markers to use (chloramphenicol, gentamycin, spectinomycin and kanamycin). In theory with this setup up to 20 genes could be introduced into a cyanobacterial strain.
Thanks to the flexible design this expansion can also be used for the genomic modification of any chassis after the introduction of new species specific LVL 0 integration sites to our Marburg Collection 2.0. As the workflow to build new homologies is a bit more intricate compared to the one pot one step assembly of our other parts due to the internal BsmbI cutting site, we described the workflow for that in our design section.
The Green Expansion proves a valuable addition to our Marburg Collection 2.0 and to the iGEM Registry of Parts. It services users of our chassis and other cyanobacterial strains with a useful tool for genomic modifications but it also contributes a shell that can be used to modify any other model organism as well.
Parts of the Marburg Collection 2.0
Download all Coding Sequences