Difference between revisions of "Team:UESTC-China/Hardware"
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| − | <p>In order to | + | <p>In order to achieve the application of our engineered bacteria in practise, we designed the expired drugs recycling and degrading device (Drug Avenger) and the fluorescence detection device.<br><br>Expired drugs recycling and degrading device (Drug Avenger) was used to recycle expired drugs. The modular design allowed for the recovery of three types of drugs. Fig. 2. and animation show the design and theoretical ideas.</p> |
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| − | <p>Fig. 1. Expired drugs recycling and degrading device | + | <p>Fig. 1. Expired drugs recycling and degrading device named Drug Avenger</p> |
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| − | <p>Fig. 2. Print of the | + | <p>Fig. 2. Print of the function module</p> |
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| − | + | In this project,the fluorescence detection device was used to specifically detect the CIP concentration, and it was assembled by a switching power supply, water pumps, acquisition card, photodetector, and an external device. | |
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| − | <p>In the development process of hardware, our detection device module | + | <p>In the development process of hardware, our detection device module had been updated three iderations, and finally the third generation version was designed and realized.</p> |
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<h2 style="font-weight: 600"><img src="https://static.igem.org/mediawiki/2019/1/1d/T--UESTC-China--arrow3.png" width="7%" alt="logo">First generation device</h2> | <h2 style="font-weight: 600"><img src="https://static.igem.org/mediawiki/2019/1/1d/T--UESTC-China--arrow3.png" width="7%" alt="logo">First generation device</h2> | ||
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| − | The first generation of detection modules consists of photodetectors, laser sources, STM32 Development board, pumps, filters, lenses, and external devices with | + | The first generation of detection modules consists of photodetectors, laser sources, STM32 Development board, pumps, filters, lenses, and external devices. The collected beam was vertical with the incident beam. |
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| − | + | After discussing with several professiors, we found that the first-generation device was not efficient in fluorescence excitation, and some details were unreasonable ,it can not meet the requirements and cost of 3D printing technology.So the second-generation device optimized the optical path based on the first-generation device in which no transparent parts were needed. The measuement angle was fixed at 45° angle to enhance fluorescence excitation efficiency. | |
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| − | We tried the second generation device and found that it | + | We tried the second generation device and found that it was not stable enough to detect the weak fluorescence. Under the guidance of Dr.Gul, we improved the original device in the fixation of the engineering bacteria, and designed the funnel-shaped groove, which was conducive to the accumulation of engineering bacteria and also improve the stability of the detection fluorescent signal. |
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| − | Finally, we designed | + | Finally, we designed the expired drugs recycling and degrading device and fluorescence detection device based on the third generation of detection module devices. |
| − | + | More detailed information can be available by clicking. <p style="color:#ff0000;display:inline">please click on the two buttons below.</p> | |
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| − | In order to make other teams | + | In order to make other teams understand it easily and improve hardware together, we share important parameters, 3D models and acquisition software below. |
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| − | <p> | + | <p> We plan to develop more functional module based on the design and theoretical ideas. We also hope to optimize the design detection module to get more stable and efficient signals. We also aim to reduce the cost of this device.</p> |
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Latest revision as of 09:40, 28 November 2019
Overview
In order to achieve the application of our engineered bacteria in practise, we designed the expired drugs recycling and degrading device (Drug Avenger) and the fluorescence detection device.
Expired drugs recycling and degrading device (Drug Avenger) was used to recycle expired drugs. The modular design allowed for the recovery of three types of drugs. Fig. 2. and animation show the design and theoretical ideas.
Fig. 1. Expired drugs recycling and degrading device named Drug Avenger
Fig. 2. Print of the function module
In this project,the fluorescence detection device was used to specifically detect the CIP concentration, and it was assembled by a switching power supply, water pumps, acquisition card, photodetector, and an external device.
Fig. 3. The fluorescence detection device
Device iteration
In the development process of hardware, our detection device module had been updated three iderations, and finally the third generation version was designed and realized.
First generation device
The first generation of detection modules consists of photodetectors, laser sources, STM32 Development board, pumps, filters, lenses, and external devices. The collected beam was vertical with the incident beam.
Fig. 4. Light path of the first generation device
Second generation device
After discussing with several professiors, we found that the first-generation device was not efficient in fluorescence excitation, and some details were unreasonable ,it can not meet the requirements and cost of 3D printing technology.So the second-generation device optimized the optical path based on the first-generation device in which no transparent parts were needed. The measuement angle was fixed at 45° angle to enhance fluorescence excitation efficiency.
Fig. 5. Light path of the second generation device
Third generation device
We tried the second generation device and found that it was not stable enough to detect the weak fluorescence. Under the guidance of Dr.Gul, we improved the original device in the fixation of the engineering bacteria, and designed the funnel-shaped groove, which was conducive to the accumulation of engineering bacteria and also improve the stability of the detection fluorescent signal.
Fig. 6. The funnel-shaped groove of the third generation device
Finally, we designed the expired drugs recycling and degrading device and fluorescence detection device based on the third generation of detection module devices.
More detailed information can be available by clicking.
please click on the two buttons below.
Expired drugs recycling and degrading device
Fluorescence detection devices
Open source
In order to make other teams understand it easily and improve hardware together, we share important parameters, 3D models and acquisition software below.
Parameters
| Input | Output | Size | Wavelength | Chip | Power | Aperture | Cut-On Wavelength | focal length | diameter | ADC | |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Photodetector | Silicon-based avalanche diode | 200~1000nm | |||||||||
| Laser | 450nm | 4.8mw | |||||||||
| Development board | 12V | STM32F103C8T6 | ADS1256 | ||||||||
| Relay | 12V | Four-way optocoupler isolation | |||||||||
| Water pump | 5V | ||||||||||
| Filter membrane | 0.22μm | ||||||||||
| Switching power supply | 220V/110V | 12V/5V | |||||||||
| optical filter | 500nm | ||||||||||
| Convex lens | 25mm | 1 inch |
Expectation
We plan to develop more functional module based on the design and theoretical ideas. We also hope to optimize the design detection module to get more stable and efficient signals. We also aim to reduce the cost of this device.