- The ciprofloxacin disposal system
The ciprofloxacin disposal system
Detection of ciprofloxacin
Degradation of ciprofloxacin
CrpP is a novel ciprofloxacin-modifying enzyme which can phosphorylate and degrade CIP . The degradation pathway is shown in Fig. 3. Under natural conditions, phosphorylated CIP will be gradually degraded and the final product 1, 4-dihydroquinoline is obtained. It has been reported that 1, 4-dihydroquinoline can be used as carriers for specific brain delivery on mice [5,6], indicating that it is valuable.
Besides, an extra signal peptide pelB followed by five aspartate repeats were introduced to facilitate the extracellular expression of CrpP in E.coli . TagRFP, a red fluorescent protein, is used as a reporter protein to response if CrpP is expressed or not.
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