Difference between revisions of "Team:UESTC-China/Hardware"

 
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<p>In order to combine the project and the progress of the experiment, we designed the community drug recycling bin and the fluorescence detection device in the hardware part.<br><br>The community drug recycling bin is used to collect and recycle expired drugs. The initial modular design allows for the recovery of three types of drugs, and the design and implementation of the detection and processing module for ciprofloxacin. Figure 1 and animation show the design and theoretical ideas of a special CIP detection module.</p>
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<p>In order to achieve the application of our engineered bacteria in practise, we designed the expired drugs recycling and degrading device (Drug Avenger) and the fluorescence detection device.<br><br>Expired drugs recycling and degrading device (Drug Avenger) was used to recycle expired drugs. The modular design allowed for the recovery of three types of drugs. Fig. 2. and animation show the design and theoretical ideas.</p>
 
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<p>Fig. 1.  Expired drugs recycling and degrading device, which we call it Drug Avenger</p>
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<p>Fig. 1.  Expired drugs recycling and degrading device named Drug Avenger</p>
 
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<p>Fig. 2. Print of the Drug Avenger</p>
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<p>Fig. 2. Print of the function module</p>
 
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<source src="https://static.igem.org/mediawiki/2019/6/63/T--UESTC-China--hardware_video.mp4" type="video/mp4">
 
<source src="https://static.igem.org/mediawiki/2019/6/63/T--UESTC-China--hardware_video.mp4" type="video/mp4">
 
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  The fluorescence detection device is used to specifically detect the CIP concentration, and is assembled by a switching power supply, water pumps, capture card, photodetector, and an external device.
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  In this project,the fluorescence detection device was used to specifically detect the CIP concentration, and it was assembled by a switching power supply, water pumps, acquisition card, photodetector, and an external device.
 
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<p>In the development process of hardware, our detection device module has been updated three times, and finally realized the design realization of the third generation version.</p>
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<p>In the development process of hardware, our detection device module had been updated three iderations, and finally the third generation version was designed and realized.</p>
 
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<h2 style="font-weight: 600"><img src="https://static.igem.org/mediawiki/2019/1/1d/T--UESTC-China--arrow3.png" width="7%" alt="logo">First generation device</h2>
 
<h2 style="font-weight: 600"><img src="https://static.igem.org/mediawiki/2019/1/1d/T--UESTC-China--arrow3.png" width="7%" alt="logo">First generation device</h2>
 
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The first generation of detection modules consists of photodetectors, laser sources, STM32 Development board, pumps, filters, lenses, and external devices with an optical path of 90°.
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The first generation of detection modules consists of photodetectors, laser sources, STM32 Development board, pumps, filters, lenses, and external devices. The collected beam was vertical with the incident beam.  
 
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The first-generation device is not efficient in fluorescence excitation, and the individual details are unreasonable because of the requirements and cost of 3D printing technology. But the second-generation device optimizes the optical path based on the first-generation device and no longer needs transparent parts. It use 45° angle to enhance the acquisition of fluorescent signals.
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After discussing with several professiors, we found that the first-generation device was not efficient in fluorescence excitation, and some details were unreasonable ,it can not meet the requirements and cost of 3D printing technology.So the second-generation device optimized the optical path based on the first-generation device in which no transparent parts were needed. The measuement angle was fixed at 45° angle to enhance fluorescence excitation efficiency.
 
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Through the communication with a doctor, we made up for the shortcomings of the original device in the fixation of the engineering bacteria, and designed the funnel-shaped groove, which is conducive to the accumulation of engineering bacteria and also improve the stability of the detection fluorescent signal.
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We tried the second generation device and found that it was not stable enough to detect the weak fluorescence. Under the guidance of Dr.Gul, we improved the original device in the fixation of the engineering bacteria, and designed the funnel-shaped groove, which was conducive to the accumulation of engineering bacteria and also improve the stability of the detection fluorescent signal.
 
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Finally, we designed a community drug recycling bin and fluorescence detection device based on the third generation of detection module devices.
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Finally, we designed the expired drugs recycling and degrading device and fluorescence detection device based on the third generation of detection module devices.
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                 For more detailed information on the design of community drug recycling bins and fluorescence detection devices, <p style="color:#ff0000;display:inline">please click on the two pictures below.</p>
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                 More detailed information can be available by clicking.  <p style="color:#ff0000;display:inline">please click on the two buttons below.</p>
 
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In order to make other teams purchase them and improve our hardware, we share important parameters, 3D models and acquisition software below.
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In order to make other teams understand it easily and improve hardware together, we share important parameters, 3D models and acquisition software below.
 
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<th>focal length</th>
 
<th>focal length</th>
 
<th>diameter</th>
 
<th>diameter</th>
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                                        <th>ADC</th>
 
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<th>Silicon-based avalanche diode</th>
 
<th>Silicon-based avalanche diode</th>
 
<th>200~1000nm</th>
 
<th>200~1000nm</th>
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<th>4.8mw</th>
 
<th>4.8mw</th>
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<th></th>
 
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                                        <th>ADS1256</th>
 
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<th>Four-way optocoupler isolation</th>
 
<th>Four-way optocoupler isolation</th>
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<th>Water pump</th>
 
<th>Water pump</th>
 
<th>5V</th>
 
<th>5V</th>
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<th>0.22μm</th>
 
<th>0.22μm</th>
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<th>220V/110V</th>
 
<th>220V/110V</th>
 
<th>12V/5V</th>
 
<th>12V/5V</th>
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<th>500nm</th>
 
<th>500nm</th>
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<th>25mm</th>
 
<th>25mm</th>
 
<th>1 inch</th>
 
<th>1 inch</th>
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<a href="#">click here to download our 3D model</a><br>
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<a href="https://static.igem.org/mediawiki/2019/7/77/T--UESTC-China--Hardware_share_1.zip">click here to download our 3D model</a><br>
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<a href="#">click here to download our software</a>
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<a href="https://static.igem.org/mediawiki/2019/c/c8/T--UESTC-China--Hardware_share_2.zip">click here to download our software</a>
 
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<p>In the future, we intend to improve the usability and extensiveness of our recycling bins by designing other drug degradation modules. We have reserved some modular designs to make them more convenient and easy to implement. We also hope to optimize the design detection module. Our signals are more stable and efficient, enabling the implementation of the device at a lower cost.</p>
+
<p> We plan to develop more functional module based on the design and theoretical ideas. We also hope to optimize the design detection module to get more stable and efficient signals. We also aim to reduce the cost of this device.</p>
 
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Latest revision as of 09:40, 28 November 2019

description

0% 完成
logo
logo

Overview

In order to achieve the application of our engineered bacteria in practise, we designed the expired drugs recycling and degrading device (Drug Avenger) and the fluorescence detection device.

Expired drugs recycling and degrading device (Drug Avenger) was used to recycle expired drugs. The modular design allowed for the recovery of three types of drugs. Fig. 2. and animation show the design and theoretical ideas.

logo

Fig. 1. Expired drugs recycling and degrading device named Drug Avenger

logo

Fig. 2. Print of the function module

In this project,the fluorescence detection device was used to specifically detect the CIP concentration, and it was assembled by a switching power supply, water pumps, acquisition card, photodetector, and an external device.
logo

Fig. 3. The fluorescence detection device

Device iteration

In the development process of hardware, our detection device module had been updated three iderations, and finally the third generation version was designed and realized.

logoFirst generation device

The first generation of detection modules consists of photodetectors, laser sources, STM32 Development board, pumps, filters, lenses, and external devices. The collected beam was vertical with the incident beam.
logo

Fig. 4. Light path of the first generation device

logoSecond generation device

After discussing with several professiors, we found that the first-generation device was not efficient in fluorescence excitation, and some details were unreasonable ,it can not meet the requirements and cost of 3D printing technology.So the second-generation device optimized the optical path based on the first-generation device in which no transparent parts were needed. The measuement angle was fixed at 45° angle to enhance fluorescence excitation efficiency.
logo

Fig. 5. Light path of the second generation device

logoThird generation device

We tried the second generation device and found that it was not stable enough to detect the weak fluorescence. Under the guidance of Dr.Gul, we improved the original device in the fixation of the engineering bacteria, and designed the funnel-shaped groove, which was conducive to the accumulation of engineering bacteria and also improve the stability of the detection fluorescent signal.
logo

Fig. 6. The funnel-shaped groove of the third generation device

Finally, we designed the expired drugs recycling and degrading device and fluorescence detection device based on the third generation of detection module devices. More detailed information can be available by clicking.

please click on the two buttons below.

Expired drugs recycling and degrading device
Fluorescence detection devices
Open source
In order to make other teams understand it easily and improve hardware together, we share important parameters, 3D models and acquisition software below.
Parameters
Input Output Size Wavelength Chip Power Aperture Cut-On Wavelength focal length diameter ADC
Photodetector Silicon-based avalanche diode 200~1000nm
Laser 450nm 4.8mw
Development board 12V STM32F103C8T6 ADS1256
Relay 12V Four-way optocoupler isolation
Water pump 5V
Filter membrane 0.22μm
Switching power supply 220V/110V 12V/5V
optical filter 500nm
Convex lens 25mm 1 inch
click here to download our 3D model
click here to download our software

Expectation

We plan to develop more functional module based on the design and theoretical ideas. We also hope to optimize the design detection module to get more stable and efficient signals. We also aim to reduce the cost of this device.

Copyright © 2019 iGEM UESTC-China
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