Difference between revisions of "Team:UESTC-China/Hardware"

 
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     <li class="active"><a href="#title_1">1.Drug Processing System</a></li>
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     <li class="active"><a href="#title_1">Overview</a></li>
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     <li ><a href="#title_2">2.Ciprofloxacin module</a></li>
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     <li><a href="#title_2">Device iteration</a></li>
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     <li ><a href="#title_3">3.Expectation</a></li>
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     <li><a href="#title_3">Open source</a></li>
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    <li><a href="#title_4">Expectation</a></li>
 
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<div class="bigtitle" id="title_1">
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<p>1.Drug Processing Box</p>
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<p>Overview</p>
 
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<div class="mainbody" >
 
<div class="mainbody" >
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<p>In order to improve the efficiency and convenience of drug recycling, we designed a community drug recycling box to collect and recycle expired drugs. The preliminary modular design can place three types of drug recycling.(Fig. 1)</p>
+
<p>In order to achieve the application of our engineered bacteria in practise, we designed the expired drugs recycling and degrading device (Drug Avenger) and the fluorescence detection device.<br><br>Expired drugs recycling and degrading device (Drug Avenger) was used to recycle expired drugs. The modular design allowed for the recovery of three types of drugs. Fig. 2. and animation show the design and theoretical ideas.</p>
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</div>
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<div style="background-color: transparent">
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<img alt="logo" src="https://static.igem.org/mediawiki/2019/8/81/T--UESTC-China--hardware1.png" class="picture">
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<div class="words" >
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<p>Fig. 1.  Expired drugs recycling and degrading device named Drug Avenger</p>
 +
</div>
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 +
<div style="text-align: center;background-color: transparent">
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<img alt="logo" src="https://static.igem.org/mediawiki/2019/5/5c/T--UESTC-China--hardware2.png" class="picture">
 
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<p>Fig. 2. Print of the function module</p>
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<div style="text-align: center">
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<video width="80%" controls preload>
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  In this project,the fluorescence detection device was used to specifically detect the CIP concentration, and it was assembled by a switching power supply, water pumps, acquisition card, photodetector, and an external device.
 +
  </div>
 +
 
 +
<div style="text-align: center;background-color: transparent">
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<img alt="logo" src="https://static.igem.org/mediawiki/2019/8/8e/T--UESTC-China--hardware3.png" width="50%">
 
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<p>Fig. 1 Drug Processing Box. </p>
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<p>Fig. 3. The fluorescence detection device</p>
 
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<div class="part">
 
<div class="part">
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<div class="bigtitle" id="title_2" style="background-color: #b6caf2">
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<p>2.Ciprofloxacin module </p>
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<p>Device iteration </p>
 
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<p>In the degradation module of ciprofloxacin, we mainly divide it into three parts: crushing, Detection and degradation; crushing module is used to crush and dissolve ciprofloxacin to improve degradation efficiency; detection module is used to detect ciprofloxacin in water; degradation module is used to degrade ciprofloxacin by our engineering bacteria, at the same time, our suicide genes cooperates with blue light. Ultraviolet light are also used in the lower part of the rear tank to ensure our Biosafety.</p>
+
<p>In the development process of hardware, our detection device module had been updated three iderations, and finally the third generation version was designed and realized.</p>
 
</div>
 
</div>
 +
 +
<h2 style="font-weight: 600"><img src="https://static.igem.org/mediawiki/2019/1/1d/T--UESTC-China--arrow3.png" width="7%" alt="logo">First generation device</h2>
 +
<div class="mainbody">
 +
The first generation of detection modules consists of photodetectors, laser sources, STM32 Development board, pumps, filters, lenses, and external devices. The collected beam was vertical with the incident beam.
 +
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<p>Fig. 2 Ciprofloxacin module  </p>
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<p>Fig. 4. Light path of the first generation device</p>
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<h2 style="font-weight: 600"><img src="https://static.igem.org/mediawiki/2019/1/1d/T--UESTC-China--arrow3.png" width="7%" alt="logo">Second generation device</h2>
 +
 +
<div class="mainbody">
 +
After discussing with several professiors, we found that the first-generation device was not efficient in fluorescence excitation, and some details were unreasonable ,it can not meet the requirements and cost of 3D printing technology.So the second-generation device optimized the optical path based on the first-generation device in which no transparent parts were needed. The measuement angle was fixed at 45° angle to enhance fluorescence excitation efficiency.
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</div>
 
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<div class="words" >
 
<div class="words" >
−
<p>Fig. 3 print of the Ciprofloxacin module.  </p>
+
<p>Fig. 5.  Light path of the second generation device</p>
 
</div>
 
</div>
−
+
−
<div style="text-align: center;background-color: #F2F3F4">
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<h2 style="font-weight: 600"><img src="https://static.igem.org/mediawiki/2019/1/1d/T--UESTC-China--arrow3.png" width="7%" alt="logo">Third generation device</h2>
−
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<div class="mainbody">
 +
We tried the second generation device and found that it was not stable enough to detect the weak fluorescence. Under the guidance of Dr.Gul, we improved the original device in the fixation of the engineering bacteria, and designed the funnel-shaped groove, which was conducive to the accumulation of engineering bacteria and also improve the stability of the detection fluorescent signal.
 +
</div>
 +
 +
<div style="text-align: center;background-color: transparent">
 +
<img alt="logo" src="https://static.igem.org/mediawiki/2019/0/0b/T--UESTC-China--hardware6.png" width="50%">
 
</div>
 
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 +
 
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<div class="words" >
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<p>Fig. 4    </p>
+
<p>Fig. 6. The funnel-shaped groove of the third generation device</p>
 
</div>
 
</div>
 
 
 
 
 +
<div class="mainbody">
 +
Finally, we designed the expired drugs recycling and degrading device and fluorescence detection device based on the third generation of detection module devices.
 +
                More detailed information can be available by clicking.  <p style="color:#ff0000;display:inline">please click on the two buttons below.</p>
 +
</div>
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        </div>
 
 
 
 
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<div class="row">
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<div class="myhar">
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Expired drugs recycling and degrading device
 +
        </div>
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 +
<div class="myhar">
 +
Fluorescence detection devices
 +
</div>
 +
</div>
 +
    </div>
 +
<div class="part">
 +
<div class="bigtitle" id="title_3">
 +
Open source
 +
</div>
 +
<div class="mainbody">
 +
In order to make other teams understand it easily and improve hardware together, we share important parameters, 3D models and acquisition software below.
 +
</div>
 +
<div class="mainbody" style="text-align:center;font-weight:600;">
 +
Parameters
 +
                </div>
 +
<div class="table-responsive">
 +
<table class="table table-bordered table-hover mainbody">
 +
<tbody>
 +
<tr>
 +
<th></th>
 +
<th>Input</th>
 +
<th>Output</th>
 +
<th>Size</th>
 +
<th>Wavelength</th>
 +
<th>Chip</th>
 +
<th>Power</th>
 +
<th>Aperture</th>
 +
<th>Cut-On Wavelength</th>
 +
<th>focal length</th>
 +
<th>diameter</th>
 +
                                        <th>ADC</th>
 +
</tr>
 +
<tr>
 +
<th>Photodetector</th>
 +
<th></th>
 +
<th></th>
 +
<th>Silicon-based avalanche diode</th>
 +
<th>200~1000nm</th>
 +
<th></th>
 +
<th></th>
 +
<th></th>
 +
<th></th>
 +
<th></th>
 +
<th></th>
 +
<th></th>
 +
</tr>
 +
<tr>
 +
<th>Laser</th>
 +
<th></th>
 +
<th></th>
 +
<th></th>
 +
<th>450nm</th>
 +
<th></th>
 +
<th>4.8mw</th>
 +
<th></th>
 +
<th></th>
 +
<th></th>
 +
<th></th>
 +
<th></th>
 +
</tr>
 +
<tr>
 +
<th>Development board</th>
 +
<th>12V</th>
 +
<th></th>
 +
<th></th>
 +
<th></th>
 +
<th>STM32F103C8T6</th>
 +
<th></th>
 +
<th></th>
 +
<th></th>
 +
<th></th>
 +
<th></th>
 +
                                        <th>ADS1256</th>
 +
</tr>
 +
<tr>
 +
<th>Relay</th>
 +
<th>12V</th>
 +
<th></th>
 +
<th>Four-way optocoupler isolation</th>
 +
<th></th>
 +
<th></th>
 +
<th></th>
 +
<th></th>
 +
<th></th>
 +
<th></th>
 +
<th></th>
 +
<th></th>
 +
</tr>
 +
<tr>
 +
<th>Water pump</th>
 +
<th>5V</th>
 +
<th></th>
 +
<th></th>
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<th></th>
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<th></th>
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<th></th>
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<th></th>
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<th></th>
 +
<th></th>
 +
<th></th>
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<th></th>
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</tr>
 +
<tr>
 +
<th>Filter membrane</th>
 +
<th></th>
 +
<th></th>
 +
<th></th>
 +
<th></th>
 +
<th></th>
 +
<th></th>
 +
<th>0.22μm</th>
 +
<th></th>
 +
<th></th>
 +
<th></th>
 +
<th></th>
 +
</tr>
 +
<tr>
 +
<th>Switching power supply</th>
 +
<th>220V/110V</th>
 +
<th>12V/5V</th>
 +
<th></th>
 +
<th></th>
 +
<th></th>
 +
<th></th>
 +
<th></th>
 +
<th></th>
 +
<th></th>
 +
<th></th>
 +
<th></th>
 +
</tr>
 +
<tr>
 +
<th>optical filter</th>
 +
<th></th>
 +
<th></th>
 +
<th></th>
 +
<th></th>
 +
<th></th>
 +
<th></th>
 +
<th></th>
 +
<th>500nm</th>
 +
<th></th>
 +
<th></th>
 +
<th></th>
 +
</tr>
 +
<tr>
 +
<th>Convex lens</th>
 +
<th></th>
 +
<th></th>
 +
<th></th>
 +
<th></th>
 +
<th></th>
 +
<th></th>
 +
<th></th>
 +
<th></th>
 +
<th>25mm</th>
 +
<th>1 inch</th>
 +
<th></th>
 +
</tr>
 +
</tbody>
 +
</table>
 
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<div class="mainbody row">
 +
<a href="https://static.igem.org/mediawiki/2019/7/77/T--UESTC-China--Hardware_share_1.zip">click here to download our 3D model</a><br>
 +
<a href="https://static.igem.org/mediawiki/2019/c/c8/T--UESTC-China--Hardware_share_2.zip">click here to download our software</a>
 +
</div>
 +
 +
</div>
 
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     <div class="part">
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<div class="bigtitle" id="title_3" style="background-color: #b6caf2">
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<div class="bigtitle" id="title_4">
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<p>3.Expectation </p>
+
<p>Expectation </p>
 
</div>
 
</div>
 
 
 
<div class="mainbody" >
 
<div class="mainbody" >
−
<p>In the future, we intend to improve the practicability and universality of our recycling bins by designing other drug degradation modules, and we have reserved some modular designs to make them more convenient and easy to implement, and hope to improve them by optimizing the design details.</p>
+
<p> We plan to develop more functional module based on the design and theoretical ideas. We also hope to optimize the design detection module to get more stable and efficient signals. We also aim to reduce the cost of this device.</p>
 
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Latest revision as of 09:40, 28 November 2019

description

0% 完成
logo
logo

Overview

In order to achieve the application of our engineered bacteria in practise, we designed the expired drugs recycling and degrading device (Drug Avenger) and the fluorescence detection device.

Expired drugs recycling and degrading device (Drug Avenger) was used to recycle expired drugs. The modular design allowed for the recovery of three types of drugs. Fig. 2. and animation show the design and theoretical ideas.

logo

Fig. 1. Expired drugs recycling and degrading device named Drug Avenger

logo

Fig. 2. Print of the function module

In this project,the fluorescence detection device was used to specifically detect the CIP concentration, and it was assembled by a switching power supply, water pumps, acquisition card, photodetector, and an external device.
logo

Fig. 3. The fluorescence detection device

Device iteration

In the development process of hardware, our detection device module had been updated three iderations, and finally the third generation version was designed and realized.

logoFirst generation device

The first generation of detection modules consists of photodetectors, laser sources, STM32 Development board, pumps, filters, lenses, and external devices. The collected beam was vertical with the incident beam.
logo

Fig. 4. Light path of the first generation device

logoSecond generation device

After discussing with several professiors, we found that the first-generation device was not efficient in fluorescence excitation, and some details were unreasonable ,it can not meet the requirements and cost of 3D printing technology.So the second-generation device optimized the optical path based on the first-generation device in which no transparent parts were needed. The measuement angle was fixed at 45° angle to enhance fluorescence excitation efficiency.
logo

Fig. 5. Light path of the second generation device

logoThird generation device

We tried the second generation device and found that it was not stable enough to detect the weak fluorescence. Under the guidance of Dr.Gul, we improved the original device in the fixation of the engineering bacteria, and designed the funnel-shaped groove, which was conducive to the accumulation of engineering bacteria and also improve the stability of the detection fluorescent signal.
logo

Fig. 6. The funnel-shaped groove of the third generation device

Finally, we designed the expired drugs recycling and degrading device and fluorescence detection device based on the third generation of detection module devices. More detailed information can be available by clicking.

please click on the two buttons below.

Expired drugs recycling and degrading device
Fluorescence detection devices
Open source
In order to make other teams understand it easily and improve hardware together, we share important parameters, 3D models and acquisition software below.
Parameters
Input Output Size Wavelength Chip Power Aperture Cut-On Wavelength focal length diameter ADC
Photodetector Silicon-based avalanche diode 200~1000nm
Laser 450nm 4.8mw
Development board 12V STM32F103C8T6 ADS1256
Relay 12V Four-way optocoupler isolation
Water pump 5V
Filter membrane 0.22μm
Switching power supply 220V/110V 12V/5V
optical filter 500nm
Convex lens 25mm 1 inch
click here to download our 3D model
click here to download our software

Expectation

We plan to develop more functional module based on the design and theoretical ideas. We also hope to optimize the design detection module to get more stable and efficient signals. We also aim to reduce the cost of this device.

Copyright © 2019 iGEM UESTC-China
logo