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Revision as of 16:55, 20 October 2019
Antibiotics are widely applied to the prevention and treatment of diseases, saving countless lives. In 2013, China consumed 92,700 tons of antibiotics, and ~58％ of them are discharged into the environment, causing serious pollution and antibiotic resistance. About 700,000 people die of antibiotic resistance each year worldwide.
Although several physical and chemical methods have been used to degrade antibiotics in the environment, such as ozonation, Fenton’s oxidation and adsorption, one cannot ignore their disadvantages, including high cost, complexity, and producing new pollution. Comparing with them, biological methods are more effective and environmentally friendly .
Antibiotic pollution not only comes from its production, such as pharmaceutical wastewater, but also origins from its usage, such as medical antibiotics , which are not far from our daily life. Every family have to face the problem of expired antibiotic drugs , but few people can realize that expired drugs are being improperly treated such as incineration and landfill.
Among antibiotics, ciprofloxacin (CIP) is commonly used and easy to cause resistance. In our project, we have constructed an engineered bacterial to effectively degrade it by introducing a CIP-induced promoter, PtisAB and a CIP-degrading protein, CrpP . Once the system senses the presence of CIP, CrpP will be produced. In this way, we achieve sensitive detection and effective degradation of CIP.
To use engineered bacteria more effectively, we built an expired drugs recycling and degrading device . We optimized this device and successfully achieved the detection of CIP by collecting GFP fluorescence emitting from engineered E.coli, which has a linear relationship with CIP present in the environment. Modelling analysis provided the guidance for the layout of this device in the social community.
In our project, we have successfully constructed the CIP-sensing and CIP-degrading system . We also developed its supporting hardware . Although more work needs to be done in the near future to optimize the system, there is no doubt that our project will provide new insight and useful guidance for bio-degradation of antibiotics.