Difference between revisions of "Team:UESTC-China/Collaborations"
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| − | This summer, we collaborated with five 2019 iGEM teams for greater progress in the project and hosted 2019 Southwestern iGEM Exchange Conference with UESTC-Software. <br> | + | This summer, we collaborated with five 2019 iGEM teams for greater progress in the project and hosted 2019 Southwestern iGEM Exchange Conference with UESTC-Software. <br><br> |
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We provided CAU_China with a plasmid and their simultaneous validation experiments. We discussed with SZU-China how to improve the feasibility of the project and the expression of toxic protein. We designed a questionnaire analysis method together with CSU_CHINA. ZJUT-China provided us with lysin. We offered a trial of our part of the last year to XMU-China . A total of five teams from Southwest China participated in the meetup we held. | We provided CAU_China with a plasmid and their simultaneous validation experiments. We discussed with SZU-China how to improve the feasibility of the project and the expression of toxic protein. We designed a questionnaire analysis method together with CSU_CHINA. ZJUT-China provided us with lysin. We offered a trial of our part of the last year to XMU-China . A total of five teams from Southwest China participated in the meetup we held. | ||
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<div class="bigtitle" id="title_4">SZU-China</div> | <div class="bigtitle" id="title_4">SZU-China</div> | ||
| − | <div class="mainbody">SZU-China tested the suicide mechanism of the toxic protein expressed by the T7 promoter this year, but they encountered many problems. For example, the endogenous proteolytic enzyme of E. coli will hydrolyze the protein after the expression of the toxic protein, resulting in a low expression of the detected protein. This year our project also designed a suicide switch to lyse E. coli. Therefore, we recommend that they use BL21 strain which was modified from DE3 to reduce the hydrolysis loss of protein. We advised them to increase the induced concentration of IPTG. We also offered new ideas. Another possible reason is that IPTG is a strong inducer, which probably makes proteins incorrectly folded because of proteins expressing too quickly, resulting in inclusion bodies and reduced protein activity. So we suggested that they could try to induce expression between 16 degrees Celsius to 30 degrees Celsius, prolong expression time, and reduce the production of inclusion bodies.<br> | + | <div class="mainbody">SZU-China tested the suicide mechanism of the toxic protein expressed by the T7 promoter this year, but they encountered many problems. For example, the endogenous proteolytic enzyme of E. coli will hydrolyze the protein after the expression of the toxic protein, resulting in a low expression of the detected protein. This year our project also designed a suicide switch to lyse E. coli. Therefore, we recommend that they use BL21 strain which was modified from DE3 to reduce the hydrolysis loss of protein. We advised them to increase the induced concentration of IPTG. We also offered new ideas. Another possible reason is that IPTG is a strong inducer, which probably makes proteins incorrectly folded because of proteins expressing too quickly, resulting in inclusion bodies and reduced protein activity. So we suggested that they could try to induce expression between 16 degrees Celsius to 30 degrees Celsius, prolong expression time, and reduce the production of inclusion bodies.<br><br> |
After hearing our thoughts on degrading antibiotics in sewage, they asked us what we planned to do to achieve it. We realized that we have not considered the application environment or going out of the laboratory. Based on their suggestions and our visits to the waterworks and sewage plants afterwards, we decided to abandon the idea of degrading antibiotics in water and change our goal to make an expired drug recycling bin instead. | After hearing our thoughts on degrading antibiotics in sewage, they asked us what we planned to do to achieve it. We realized that we have not considered the application environment or going out of the laboratory. Based on their suggestions and our visits to the waterworks and sewage plants afterwards, we decided to abandon the idea of degrading antibiotics in water and change our goal to make an expired drug recycling bin instead. | ||
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Revision as of 13:51, 16 October 2019
Overview
This summer, we collaborated with five 2019 iGEM teams for greater progress in the project and hosted 2019 Southwestern iGEM Exchange Conference with UESTC-Software.
We provided CAU_China with a plasmid and their simultaneous validation experiments. We discussed with SZU-China how to improve the feasibility of the project and the expression of toxic protein. We designed a questionnaire analysis method together with CSU_CHINA. ZJUT-China provided us with lysin. We offered a trial of our part of the last year to XMU-China . A total of five teams from Southwest China participated in the meetup we held.
We provided CAU_China with a plasmid and their simultaneous validation experiments. We discussed with SZU-China how to improve the feasibility of the project and the expression of toxic protein. We designed a questionnaire analysis method together with CSU_CHINA. ZJUT-China provided us with lysin. We offered a trial of our part of the last year to XMU-China . A total of five teams from Southwest China participated in the meetup we held.
Host the meetup
On 3rd May 2019-4 May 2019, UESTC-Softerware and we (UESTC-China) hosted 2019 Southwestern iGEM Exchange Conference in order to encourage the development of iGEM in the west of China. BM-AMU, SCU-China, SICAU-iGEM, XJTU-China, SCU-China participated in this meeting. We also invented Asian Ambassador Dorothy Zhang. She anylysed the difference of the rules for new medal criteria between this year and before, and introduce the After iGEM for us in the meetup. She told us human practices should follow social science. Each team made a presentation to show their amazing ideas.
click here to see more
Asian Ambassador Dorothy Zhang
BM-AMU
XJTU-Chian
UESTC-China
UESTC-Software
SCU-China
poster
SZU-China
SZU-China tested the suicide mechanism of the toxic protein expressed by the T7 promoter this year, but they encountered many problems. For example, the endogenous proteolytic enzyme of E. coli will hydrolyze the protein after the expression of the toxic protein, resulting in a low expression of the detected protein. This year our project also designed a suicide switch to lyse E. coli. Therefore, we recommend that they use BL21 strain which was modified from DE3 to reduce the hydrolysis loss of protein. We advised them to increase the induced concentration of IPTG. We also offered new ideas. Another possible reason is that IPTG is a strong inducer, which probably makes proteins incorrectly folded because of proteins expressing too quickly, resulting in inclusion bodies and reduced protein activity. So we suggested that they could try to induce expression between 16 degrees Celsius to 30 degrees Celsius, prolong expression time, and reduce the production of inclusion bodies.
After hearing our thoughts on degrading antibiotics in sewage, they asked us what we planned to do to achieve it. We realized that we have not considered the application environment or going out of the laboratory. Based on their suggestions and our visits to the waterworks and sewage plants afterwards, we decided to abandon the idea of degrading antibiotics in water and change our goal to make an expired drug recycling bin instead.
After hearing our thoughts on degrading antibiotics in sewage, they asked us what we planned to do to achieve it. We realized that we have not considered the application environment or going out of the laboratory. Based on their suggestions and our visits to the waterworks and sewage plants afterwards, we decided to abandon the idea of degrading antibiotics in water and change our goal to make an expired drug recycling bin instead.
CSU_CHINA
When we encountered difficulties in the questionnaire analysis, we decided to conduct a video discussion with the students of CSU_CHINA to analyze the statistics in the questionnaire we posted. They suggested that we use SPSS analysis, and analyze multiple factors together in the analysis process. We also discussed with them how to carry out public participation on the elderly. They told us about their square dance activities in 2018. They said that although they did a good job, they did not do well in the news. As a result, they were not recognized by the judges during the presentation, and they suggested that we do more in the news promotion.
