Difference between revisions of "Team:UESTC-China/Hardware1"

In order to utilize our engineering bacteria to degrade ciprofloxacin in expired drugs, we designed a community drug recycling treatment tank in combination with the third generation fluorescence detection module.
In order to make it better adapt to more application scenarios and easy to maintain, we adopt a fully modular design, which is mainly divided into two large modules: water tank module and function module. The function module can be further divided into three small modules: a frame module, a smash module, a detection module, and a degradation module.
We also designed two biosafety lines to ensure the safety of our equipment.

We designed the pulverizing module with reference to the principle of the shredder. It is mainly composed of a combination of a cover, a pulverizing wheel, a water pump, a filter membrane and the like. Our community drug recovery treatment tanks have the highest priority in the treatment capacity of the device. The ph condition of the mobile phase in the whole device is the best working ph of the crpp enzyme, which may result in low dissolution efficiency of ciprofloxacin in the drug. This in turn affects the operation of the device, and our shredding module buffers and solves this problem. After entering the pulverizing module, the tablets or capsules are first pulverized into powder by the pulverizing wheel, and then the pump continues to flush the water to dissolve the powder. The undissolved powder will be left behind by the filter until it is dissolved, and the dissolved ciprofloxacin will flow in. The next module.

The detection module will act as a sensor for the entire community drug recovery treatment tank to control the operation of equipment such as pumps in the tank. By placing the test bacteria we produced in the cartridge module in the detection module, we can detect the ring C from the crushing module. Sand star, and release AHL to the downstream degradation module to open the expression of crpP. By detecting fluorescence, the detection module can determine whether the water from the comminution module contains the opening and closing of a device such as a ciprofloxacin-controlled water pump. The whole functional module is divided into three layers. In addition to loading the detection module, the second layer reserves a lot of space. In the future, according to the actual situation, the layer can also be equipped with a small water tank for holding the detection bacteria to provide more AHL.

The degradation module is mainly composed of a water tank and a pipeline. The water tank is filled with LB to supply degrading bacteria in the pipeline. The pipeline is composed of a bracket and a dialysis bag, and the inside of the pipeline is filled with a degrading bacteria embedded by sodium alginate. Small ball. The water containing ciprofloxacin and AHL from the upper layer will pass through the pipeline, and AHL will induce the degrading bacteria to express crpP to degrade ciprofloxacin. Finally, the treated water in the pipeline will be transported to the water tank by the pump under the frame module. The lower layer of the module.

In the layer where the degradation module is placed in the frame module, two bright blue light tubes are installed to keep them alive by using the suicide gene in the degrading bacteria. Once the degrading bacteria in the pipeline leave, they will not receive blue light. death.

The water tank module is used to supply water to the functional module and to dilute, process and discharge the waste water from the functional module. The water tank module is divided into two layers, wherein the upper layer is used to supply water to the functional module, and the lower layer is provided with a dark room to suicide the degrading bacteria, and the lower layer is also UV lamp can be installed for secondary sterilization to ensure the safety of the device