Team:Xiamen City/Improve
Fusion signal peptide with GFP
Signal peptide
A signal peptide (sometimes referred to as signal sequence, targeting signal, localization signal, localization sequence, transit peptide, leader sequence or leader peptide) is a short (5-30 amino acids long) peptide present at the N-terminus of the majority of newly synthesized proteins that are destined towards the secretory pathway. If we found the protein(s) which are able to degrade m-phenylenediamine, we would try to secret the protein(s) out of the cell to improve the efficiency of degradation.
Here is signal sequences list [1]:
We use these signal peptides infusion with GFP: LPPGFP, LTBGFP, OmpAGFP, OmpCGFP, OmpFGFP, OmpTGFP, PelBGFP, PhoAGFP, PhoEGFP.
2.The usage of laccase
Laccase is a member of the polycopper oxidation family. It may be able to catalyze and oxidize m-phenylenediamine. It is widely used in many industries, including food manufacturers, paper mills, textile mills, paints factories, and organic chemical factories. Our team constructed a plasmid containing laccase gene fragments and transformed it into Escherichia coli MG1655 strain (and other strains) to expressed in E. coli competent cells. In follow-up experiments, we completed the transformation of the recombinant plasmid and verified its inhibitory effect and degradation on m-phenylenediamine (MPD).
Previous projects using laccase, for example, iGEM team iGEM10_Tianjin, iGEM12_Bielefeld-Germany, Slovenia_HS, CHINA_CD_UESTC. They used laccase to oxidize phenolic compounds or other synthetic substrates.