Team:Worldshaper-XSHS/Applied Design
Applied Design
We designed a small three-dimensional compact version of the purifier, the uppermost cuboid is the azo reductase reaction cell, the instrument's first degradation process - breaking the azo bond into a slightly toxic aromatic amine compound, The second degradation line is the laccase reaction cell (laccase sclac) (shown in Figure 1 to Figure 5). The third reaction cell is a laccase reaction cell for re-degrading azo wastewater that is not up to standard.
figure1. Product appearance
figure2. 3D print model
figure3. 3D print model
figure4. 3D print model
Since azoreductase needs to operate normally in an anaerobic environment, we put a strong reducing substance (sodium dithionite solution) into the azoreductase reaction cell to remove oxygen.
Use steps and processes
1 Close and tighten all the cocks, and fill each reaction tank with the corresponding bacterial liquid. Insert 2mmol sodium dithionite solution into the first reaction tank on the left side of the first reaction tank to make an oxygen-free environment.
2 Extract the wastewater to the funnel and inject the wastewater into the funnel according to the fixed water flow rate until the maximum capacity of the first reaction tank is reached.
3 Waiting for the wastewater to completely enter the first reaction tank to stop the water injection. After the reaction time of 48 hours, the first cock is opened, and the liquid flows into the second reaction tank for 48 hours.
4 Open the second cock and inject a proper amount of liquid into the azo concentration meter if it meets the standard.
Direct discharge, if not up to standard, then enter the third reaction tank for the third re-reaction
The emission is carried out until the concentration reaches the standard.
Device advantages
The 3D printing material is PLA polylactic acid, which is a new type of bio-based and renewable biodegradable material made from starch raw materials proposed by renewable plant resources (such as corn, cassava, etc.). It has good biodegradability. After use, it can be completely degraded by microorganisms in nature under certain conditions, and finally produces carbon dioxide and water without polluting the environment. It is a recognized environmentally friendly material.
The device has a simple process and strong maneuverability, and if it is expanded to several tens of times, it can be put into industrial production.
References:
Application of sodium dithionite in establishing an anaerobic environment for cultured cells Article ID: 1000-5404(2005)04-0359-02
Authors Xu Wei, Wang Peiyong, Ma Hongying