Team:Shanghai City/Parts

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Megamenu

GEEnager: Gene Engineering and Encryption team

Our team aims to encipher message into DNA subsequence and decipher the message by Cas12a protein, primers and PCR technology, which gets a lot advantages including high storage safety, large storage space and long storage time.

PARTS

Usage and meaning

First, let’s see a picture.
Reference: Zakeri, B., Carr, P. A. & Lu, T. K. Multiplexed Sequence Encoding: A Framework for DNA Communication. PLoS One 11, e0152774, doi:10.1371/journal.pone.0152774 (2016).

Obviously, it’s a keyboard, but every sign and letter are translated into the sequence of ATGC. That’s the point of our parts. In our experiment, we see the DNA parts as a form or a language like the binary system and Morse. We translate the information that we want to express into these sequences. Our topic is DNA encoding, by using this parts, we can not only encode the message in a biological way, but also perfectly hide the message itself, for example, we just need to put it into a sequence with random order, because no one knows where is the start of the message, they could hardly break it out.

It is predicted that there will be 44ZB information in the world in 2025. It is enough that the purity of silicon on the earth to reach the storage level will not be enough to store this information. DNA storage technology can store extremely large amounts of data in grams, milligrams or even micrograms. In the DNA sequence, in the DNA storage technology, the text information can create the DNA sequence of the ATGC four bases instead of the letters by creating a comparison table, and if the data is stored, the binary number is replaced with the ATGC base sequence. Wait. According to calculations in the literature, only 1 kg of DNA can be stored in the world. In addition to the large amount of information that can be stored, DNA storage technology can store data for a very long time. According to the characteristics of DNA, DNA can be stored for at least a hundred years under normal circumstances.

This part is DNA sequence encoded a secret message. This sequence can be translated into a senstence: IGEM!SHANGHAI_CITY IS COMING!

This part is DNA sequence encoded a secret message. This sequence can be translated into a senstence: We in 2019 at iGEM win

This part is DNA sequence encoded a secret message. This sequence can be translated into a senstence: Shanghai_city in 2019 at iGEM is winner

This part is DNA sequence encoded a secret message. This sequence can be translated into a senstence: Egg man in evening in swimming pool beats the monster

This part is DNA sequence encoded a secret message. This sequence can be translated into a senstence: The male that night in a dull street murders a female

This part is DNA sequence encoded a secret message. This sequence can be translated into a senstence: A cat in a hot day in the field eats a mouse

This part is DNA sequence encoded a secret message. This sequence can be translated into a senstence: Blackhole in the future in milky way swallow the solar system

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