Team:Shanghai City/Improve
Improve
When we searched the previous iGEM team, we found several iGEM teams involving DNA storage as their project. See the following table.
Our iGEM team is dedicated to DNA encryption and storage of information and secret communication. The following four levels describe our topic and compare it with previous technologies.
1. The first encryption level is the combination of DNA encryption and computer technology. How do you translate that information into the sequence of DNA (ATCG)? We adopted the method corresponding to the password table or further strengthened combined with computer science. We also designed a 4W (who, where, when, what) one-step information assembly method similar to golden gate.
2. The second level is the application of steganography. Clelland et al. first used biosteganalysis to hide a meaningful piece of DNA in genomic DNA, then decoded it by polymerase chain reaction (PCR) using a pair of primers (keys), and then sequenced. We also tested this.
3. The third level is encryption or steganography of the secret key (in this case, the Primer) itself. (1) Encryption: we perform asymmetric secret key encryption similar to computer Encryption on two primers.(2) Steganography: on the basis of CADS method (long primers), add more complex information interference items, including single and double stranded DNA, etc.
4. The fourth level is the preservation of DNA. The DNA is stored on paper, making it harder for a thief to decipher. As far as I know, this is the first time DNA has been used for encryption on paper.