Team:SHSBNU China/Collaborations

• Acknowledgement
• Team
  Team Members Our School Collaborations Attributions
• Judging
  Judging Achievements
• Model
  Modeling Software
• Safety
• Human Practices
  Intergrated Human Practices Public Engagement
• Parts
  Overview Basic parts Composite Part Collection Improve
• Lab
  Experiments Notebook Protocols
• Project
  Inspiration Description Design Results Hardware Demonstrate Prospect

Collaborations

I. With BHSF_ND

Received arabinose-induced pSB4K5-sfGFP with resistance of Kanamycin

During the starting time of our experiment, Our team intended to test the feature of the GFP mutants, so we initially integrated sfGFP to a lac-induced BioBrick with resistance of Ampicillin. Since Ampicillin cannot effectively select the bacteria with ideal plasmid and our designed promotor of the PROBE plasmid is lac-induced, we decided to offer help from BHSF_ND since they had already developed their BioBrick which was also ideal for us.

Communication: Cross monitor in lab work and project topic sharing

Both of our teams receive the support of lab field and equipment from Bluepha Co. Ltd, and we are neighbors in the lab in PKUCare Industrial Park. Therefore, we sometimes used same equipment such as PCR instruments, nanodrop, centrifuges, etc. We always cross check the usage of the equipment since we sometimes will have some fundamental mistakes when doing lab, which would even ruin the progress of the day, when we were making biobricks. Without each other, the persistence of extracting plasmids and demonstrating the work of the systems will be much longer than expected. In our spare time, we became better known with each other by introducing our projects and talking about the topics of the protocols of experiment in order to be more efficient in lab.

II. With BNDS_China

Modelling Inspiration and cross-check

Since we did not have any inspiration of what we were going to do for modelling, we received generous help from BNDS_China for sharing the resources for learning some principles of modelling, which inspired us to construct our model. Our team had done a good job in our modelling, and we have cross-checked our models when we have all finished.

Communication : Cross monitor in lab work and project topic sharing

Both of our teams receive the support of lab field and equipment from Bluepha Co. Ltd, and we are neighbors in the lab in PKUCare Industrial Park—we even shared the same working table. We offered help to BNDS_China when they found they did not have reporter gene for biobrick characterization by sharing our RGP-sfGFP to them. We ate lunch, and sometimes dinner, together to review the process of making our biobricks, including the usage of the equipment—PCR instruments, nanodrop, centrifuges, super clean bench, even pipettes, alcohol burner, gel electrophoresis instrument, etc—and process in protocols. Without them, there would be more problems in the process of constructing our mutant GFP genes or make the whole experiment longer since some of our members always forget the crucial part of extracting plasmids or transforming the competent cells, which needed monitor and help to some extent. We made the collaboration better by introducing the topics with each other, which would make everyone know the progress and make each other easier for monitor and cross-check. We also participated in the activity of “More than a Bulletin Board” in their human practices to provide information.

III. With BNU-China

Suggestions of Project Goal

Since we are all making products that are intended to be used in the digestive system, we seek help form them when we get some uncertainties about if PROBE will work or not in the system when putting into application. We intended to make the signal to be quantitively applicable at first, but we knew from BNU-China that the task will be much more difficult than expected. We have learnt that the intestinal system is so complicated that no one could claim the factors influencing the growth and proper work of any detection on the intestinal system is fully known. Eventually, the help of collaboration from BNU-China leads us to design our hardware—the capsule. Learn more information about this in our Human Practice!