Team:SHSBNU China/Attributions

• Acknowledgement
• Team
  Team Members Our School Collaborations Attributions
• Judging
  Judging Achievements
• Model
  Modeling Software
• Safety
• Human Practices
  Intergrated Human Practices Public Engagement
• Parts
  Overview Basic parts Composite Part Collection Improve
• Lab
  Experiments Notebook Protocols
• Project
  Inspiration Description Design Results Hardware Demonstrate Prospect

Attributions

Project Design

We designed a capsule which contains the bacteria that record DNA signals. It can sense chemicals of intestinal environment by bacteria within it. Patient can use a magnet to recover this capsule and send it back to the hospital for further analysis. Then the doctor will analyze the expression intensity of GFP to determine the severity of IBD.

Lab

sfGFP Site Mutation

Mutation Site Design: Tingzhen Liu
Primer Design: Hang Cao, Qiongdan Zheng, Sicheng Jing. Yanchao Zhang, Yunmai Wang, Haocong Li
Molecular Cloning: Hang Cao, Muchen Liu, Qiongdan Zheng, Yanchao Zhang, Yunmai Wang, Haocong Li

PROBE based on SCRIBE

Design: Primer Design: Hanxiao Zhang
Splice: Hanxiao Zhang, Zhiqing Wang
Add sfgfp repair fragment: Tingzhen Liu, Zhiqing Wang, Jiawen Zou, Haocong Li.
Construction of sfgfp plasmid controlled by arabinose: Tingzhen Liu, Zhiqing Zou, Hanxiao Zhang
Construction of scribe diplasmid: Tingzhen Liu
Scribe qualitative testing: Haocong Li, Tingzhen Liu
Scribe quantitative testing: Zhiqing Wang, Tingzhen Liu
CsgA part: Tingzhen Liu, Zhiqing Wang, Xinrui Li

Human Practices

Communication with BNU.
Communication program: Haocong Li, Tingzhen Liu, Jiawen zou, Hanxiao Zhang.
Oral presentation: Haocong Li.
Communication with CCIC
Communication program: Kangxuan Rong, Tingzhen Liu, Hanxiao Zhang
Ppt poster lecture: Kangxuan Rong, Tingzhen Liu, Hanxiao Zhang
Communication with 301 Hospital
Communication program: Yanchao Zhang, Tingzhen Liu, Sicheng Jing
Communication with Naval general hospital
Communication program: Yanchao Zhang, Tingzhen Liu, Sicheng Jing
Communication with CSPC pharmaceutical factory
Communication program: Shuhan He, Hang Cao, Sicheng Jing, Jiawen Zou, Tingzhen Liu

Model

Design: Hanxiao Zhang, Pingzheng Wang
Background: Hanxiao Zhang, Pingzheng Wang
Reduction: Hanxiao Zhang, Pinzheng Wang
Result: Pinzheng Wang
Plotting: Hanxiao Zhang, Pingzheng Wang

Art Design

Qiongdan Zhang: Wiki layout, drew wiki illustration, designed team uniform, designed team logo and emblem.
Kangxuan Rong: drew wiki illustration, designed banner and poster.
Muchen Lyu: Drew wiki illustration, designed team logo

Wiki Construction

Coding: Yunmai Wang, Yanchao Zhang, Kangxuan Rong, Tingzhen Liu, Haocong Li
Designing: Qiongdan Zhang, Muchen Lv
Everyone did the attribution for the content of Wiki.

Experiments after Aug.31

PROBE-CAMERA: Ruijuan Xiang Yihao Zhang
BBa_K5316888: Chuan Ni, Tingzhen Liu, Kangxuan Rong, Hang Cao
Flow Cytometry Tests: Yihao Zhang(teach us how to use the machine) ,Tingzhen Liu , Zhiqing Wang.

Hardware

Design:Tingzhen Liu
Design guide: Chuan Ni

Pre-iGEM Training & Project Start:

Before the start of our project this spring, the synthetic biology club of our school (Teacher: Chuan Ni) provided us entry level training on genetic engineering since last autumn. The courses can be viewed freely by our students at school on the campus learning website. Bluepha Lab provided us a winter camp activity at the beginning of this year, and this shaped our project further by promoting us to brainstorm our project in prototype. During the whole process of our project, Yihao Zhang and Ruijuan Xiang gave us in-depth advice of bi">