Team:OUC-China/Collaborations
WHAT HAVE WE DONE？
★ Ideal lab
A huge challenge came this summer - our team's laboratory was relocated and revamped! So we began to think about how to design synthetic biology laboratories. We have published an invitation to cooperate on iGEM's official website-“What is your dream synthetic biology laboratory?”
We look forward to receiving some pictures and descriptions of the iGEM team's existing laboratories, which will provide us with ideas on how to design laboratories; in addition, we hope that more iGEM teams will be able to draw Ideal lab's laboratory drawings, which we hope will become "the greatest inspiration of mankind" and "the future of synthetic biology laboratories".
Fortunately, many teams participated in our cooperative projects, which inspired our ideas!
★ Comic Book
Ocean University of China published a comic book on synthetic biology in 2017. In 2018, the E. coli SPACESHIP comic book series began to cooperate on the iGEM official website and achieved high praise. This year, our team expanded the content of the comic book and invited more foreign teams to help us diversify the language of the comic book. At the same time, we cooperate with many domestic and foreign teams, and we are very honored that they are willing to use our comic book to promote the science of synthetic biology. It is worth mentioning that these teams also give us some good feedback about the effects of comic book E.coli SPACESHIP. we hope everyone to actively participate in science popularization, achieving the purpose of improving the depth and breadth of science popularization, strengthening the inheritance of synthetic biology.
★ Part measurement
In order to verify the universality and feasibility of the modular riboswitch constructed by our team in practice, we need to express our sequence in different experimental environments to explore the stability of this structure. We cooperated with some universities in the laboratory. They helped us test the modular Adda riboswitch. The feedback experiment results show that our design is stable and useful. In addition, we have helped some universities test their systems and gave them a good feedback！
★ Meet Up
This year, we paid attention to the communication between teams, made many friends, exchanged project experience and got valuable suggestions. We benefit from each other!
1. We hosted an offline meeting with SDU. We exchanged our project designs and gave each other some suggestion.
2. We hosted an online meeting with AHUT. AHUT iGEMers shared the design of their lab and ideal lab. In addition, we exchanged projects with each other.
3. We participated in the meetings of iGEM Chongqing Meet Up
4. We participated in iGEM Beijing Meet Up
5. We participated in the Seminar on Synthetic Biology Young Scholars
6. We participated in the 6th Conference of China iGEMer Community
★ Model
This year, we are making colloborations with HBUT about modeling work. Knowing that they are doing the job about the NiCoT transporter, we are going to make a molecular dynamic simulation of the process that Nickle ion entering the cell through the NixA transporter protein.
★ Mini-lab
In order to enable the public to experience the scientific life, we have created a "mini lab", expecting anyone who is interested in microbial and molecular experiments to join us! We shared our microlab with SDU-China team and they gave us great advice and feedback!
TEAM LISTS
Click team name to see our collaboration！
This year, we have maintained contact with the SDU-China team. Our team members gave a lot of advice when they set up the team, and invited them to our campus to participate in the 2018 iGEM "Shan Hai" experience sharing session held by our team. We not only shared the experience of the meeting, but also shared some new ideas of human practice. More importantly, the SDU-China team experienced our “mini- lab”! They gave us great advice and feedback.
In order to verify the feasibility of the modular riboswitch constructed by our team in practice, we need to express our sequence in different experimental environments to explore the stability of this structure. NEU_China's iGEMer helped us to verify the repeatability of the modular Adda riboswitch. We chose to analyze the data measured after 8 hours of incubation and get that the fluorescence/RFU values of the groups of 2-AP (ligand) added with different concentrations (0, 50 μM/ml, 150 μM/ml) are obvious difference. Therefore, the experimental results show that our system can work normally in different laboratory environments, and the system is feasible.
Figure 1. The effect of modular riboswitch with different concentrations of 2-AP. Fluorescence/RFU values of the groups of 2-AP (ligand) added with different concentrations (0, 50 μM/ml, 150 μM/ml) are gained after 8 hours’ inducing.
In terms of "Ideal Laboratory", we had an online discussion with iGEMers from AHUT_China, who provided us with their existing laboratory layout and what they thought would be the design of future synthetic biology laboratories. We marvel at their talent and wisdom!
To verify the feasibility of our riboswitch, we need to express our sequence in different environments to explore its stability. AHUT_China's iGEMer helped us to verify the repeatability of the modular Adda riboswitch. We chose to analyze the data measured after 8 hours of incubation and get that the fluorescence/RFU values of the groups of 2-AP (ligand) added with different concentrations (0, 50 μM/ml, 150 μM/ml) are obvious difference. Therefore, the experimental results show that our system can work normally in different laboratory environments, and the system is feasible.
What is more, AHUT_China's iGEMers are interested in our comic book and use it in their scientific popularization of synthetic biology to the public.
To verify the feasibility of the modular ribosome switch constructed by our team in practice, we need to express our sequences in different experimental environments to explore the stability of the structure. With the help of NEFU_China iGEMer, we have successfully verified the repeatability of the modular Adda riboswitch. Analyzing the data measured after 8 hours of incubation and get that the fluorescence/RFU values of the groups of 2-AP (ligand) added with different concentrations (0, 50 μM/ml, 150 μM/ml) are obvious difference. The experimental results show that our system can work normally in different laboratory environments, and the system is successful.
Fig:The fluorescence/RFU values after eight hours of the groups of 2-AP (ligand) added with different concentrations (0, 0.05 mM, 0.15 mM)
This year, we have established a closer working relationship with the SZU_China team. We visited their team and talked about our project. Then we were invited to visit their Laboratory and record the arrangement of the Laboratory, which is of great significance for our Ideal Laboratory. Finally, we offered SZU_China team in Chinese and English versions of the e. coli SPACESHIP comic book series, which will be used in them to the public for synthetic biology science popularization, the corresponding they gave us a detailed feedback and Suggestions.
In order to verify the feasibility of the modular riboswitch constructed by our team in practice, we need to express our sequence in different experimental environments to explore the stability of this structure. Jilin_China iGEMer helped us to test the repeatability of the modular Adda riboswitch. We chose to analyze the data measured after 8 hours of incubation and get that the fluorescence/RFU values of the groups of 2-AP (ligand) added with different concentrations (0, 50 μM/ml, 150 μM/ml) are obvious difference. Therefore, the experimental results show that our system can work normally in different laboratory environments, and the system is feasible.
On July 20 to July 21, We were invited to the chongqing meeting, where six teams Shared projects, ideas, Suggestions and had a good time.We were greatly inspired by the brilliant ideas of the other iGEM teams and benefited from their Suggestions.
On Aug 6th, BIT-China iGEM team invited UCAS-China and other 6 undergraduate teams to attend a meet-up, including Peking, Tsinghua-A, Tsinghua, OUC-China, CAU-China and BNU-China. During this meetup, eight teams gave presentation of their projects, shared ideas, put forward suggestions and had a great time. We were inspired greatly by the fantastic ideas from other iGEM teams and also benefit a lot from their suggestions.
In terms of "Ideal Laboratory", William and Mary provided us with their existing laboratory layout and what they thought would be the design of future synthetic biology laboratories, They hope that the ideal lab should have independent space to hold the mammalian cell culture, noisy equipment and fluorescence microscope, they also hope that the future can have incorporate robotic liquid handlers, an array of microfluidic devices, and 3D cell printers. We think their future lab will be a great engineering lab for synthetic biology!
This year, we have established a closer relationship with the Thessaloniki team. We handed the newly created E. coli SPACESHIP comic series to the Thessaloniki team and translated them into Greek and used them in their human practice activities. They gave us a good feedback!
This year, we have established a closer relationship with the NAU-CHINA team. We handed the comic series to them and they gave us a good feedback!
This year, HBUT-China iGEMers brought our comic——“SPACESHIP E.coli” and promote it to the children in the village.
What is more, we help them with a model instruct
Clickhereto see detail.
The 2019 Strasbourg iGEMers are trying to creat a test allowing the detection of food allergens and want to do a survey on the impact and incidence of allergies in different countries. At their invitation, we filled in the Survey for Strasbourg iGEM Team!
This year we exchanged the manual with FAFU-CHINA! We used each other's manuals to do HP's promotion activities, and gave good feedback and some suggestions. We believe that this cooperation is meaningful and he can make us progress together. In addition, FAFU-CHINA also provided us with photos of the laboratory!
This year, SEFLS_Shanghai joined our comic book program! He translated our E. coli series comic book into Japanese and promoted it to the school's biology teacher. They will teach sixth-grade children to learn biology by using these comic books. We are excited about their enthusiasm!
This year, the Tartu_TUIT team sent us photos of the existing labs in their labs and great ideas for future labs. The following are their photos and great ideas!
“Right next to our lab is the shaker room with shakers of different sizes to mix, blend or agitate substances.
Coming to our own small lab, we have centrifuge, vortex and a gel electrophoresis system that we are consistently using for our own experiments. In addition to these, we have pipettes, tips, Eppendorf tubes and gloves of different sizes as well. The room has several shelves too. A computer, fridge and sink are also present.”
“Even though our already existing lab is pretty much amazing, there are a few more interesting things that will make our lab close enough to an ideal DREAM lab . For instance, it would be perfect to have an air conditioner for fighting the heat on the blazing summer days of Tartu. Also with the upcoming advancements in technology, the idea of having a robot which can make PCR mixes automatically or pipetting robots which can do pipetting instead of humans does not seem like a bad idea.A programmed lab that can run experiments by our comments and voices just sounds perfect even though it seems unachievable at this point. A robot that can recognize contaminated tips and segregate them according to their size to make the autoclaving process a little less tedious would be good too. A closed space for recreation(like gym, television or music) between the tiresome lab work along with a place to eat somewhere near the labs would be great as it would help keep an eye on the experiments as well as give us time to relax and fill our bellies with food. Coming to the topic of machines that we would really like to have, the E-Gel Power Snap Electrophoresis System comes at the top of the list because we run way too many samples in a day. Although a gradient PCR machine exists in the big lab, it would be useful to have it in the small lab as well. Automated pipettes for unsurpassed ease of operation for serial dilutions as well as multichannel pipettes would be amazing to have in the lab. The CoolCube Microtube and PCR Plate Cooler that keeps samples cool and safe on the lab benchtop without degradation caused by temperature increase or fluctuation will be useful in protecting our samples.”
We are amazed at the excellent and avant-garde imagination of the Tartu_TUIT team. Maybe the future labs will become more automated and intelligent as they say, and scientists will have more time to design fantastic experimental reactions instead of repeating. Working mechanically, you will have a more personal space to relax, and you will have a smarter experimental environment to protect your samples, and you will have a more scientific and efficient experimental machine. All in all, this is undoubtedly a great laboratory that is wonderful and expects to be realized, and we are very willing to look forward to the future of the synthetic biology laboratory.
Phage infection occurs accidentally not only in the fermentation factories but also in the lab, and it might get worse and worse without appropriate treatment. By coincidence, we met team JiangnanU_China in the CCiC. Having heard their anti-phage project, we wanted to figure out whether our E.coli strain encountered a phage infection. They sent the script of their booklet to us, and we easily checked that the death strain was not caused by phage. Meanwhile, we together improved the PICP (Prevention, Identification, Control of Phage) booklet, allowing more teams to maintain a phage-free lab environment.
