Team:OUC-China/Experiments

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This year, our project can be divided into four systems. Between the different systems, there are different protocols. And we have sorted out these experimentals in detail.



1.ELEMENTARY OPERATION


（1）Conservation

（2）Gel Electrophoresis

（3）Inoculation

（4）Medium

（5）Plasmid extraction

（6）Colony Polymerase Chain Reaction

（7）Preparative Methods of Competent Escherichia coli

（8）Transformation of Escherichia coli







2.SYSTEMS DETECTION


（1）Assay of Gene Expression by Flow Cytometry

（2）Assay of Gene Expression by Laser Confocal Microscope

（3）Fluorescence measurement