Team:Nanjing/Composite Part

Composite Parts

We combine the gene Ghchi6 with the plasmid pCAMBIA2301 cloning vector to make a connecting product. This product is furtherly transferred to the E.coli to propagate the plasmid along with our gene of interest.

This is the illustration of our cloning vector. We firstly add restriction sites EcoRI and HindIII onto our gene of interest. Then we use double digests method to transfer the gene onto the cloning vector. Our final composite part is the combination of gene Ghchi6 and plasmid pCAMBIA2301.