Team:NTHU Taiwan/Parts
Overview
This year, we designed 20 new BioBricks to build FarFarmIA. To construct our tunable-temperature-sensitive-system (TTSS), we use lipase from different bacteria and design various fatty acid promoter to find out which promoter is better and is more suitable for our system. Furthermore, we also design lots of fatty acid sensitive promoters to coordinate with different fatty acid concentration to regulate downstream expression level. Finally, we use the urease as our downstream to convert human waste into ammonium. ( see more details in Project/Design)
Lipase Related Parts
| Name | Type | Description | Structure | Length |
|---|---|---|---|---|
| BBa_K3040000 | Composite | Expressing of Lipase from Pseudomonas sp 7323. | Promoter-RBS-Lipase-Pseudomonas sp 7323-6x histag | 2115 bp |
| BBa_K3040001 | Composite | Expressing of Lipase from Psychrobacter sp 7195 | Promoter-RBS-Lipase-Psychrobacter sp 7195-6x histag | 1472 bp |
| BBa_K3040002 | Composite | Lpp-OmpA | Promoter-RBS-Lpp-OmpA-6X histag | 704 bp |
| BBa_K3040100 | Basic | Lipase from Pseudomonas sp 7323. | Lipase-Pseudomonas sp 7323. | 1851 bp |
| BBa_K3040101 | Basic | Lipase from Psychrobacter sp 7195. | Lipase-Psychrobacter sp 7195. | 954 bp |
| BBa_K3040102 | Basic | Lpp-OmpA- | Lpp-Ompa | 435 bp |
Fatty Acid Promoter Related Parts
| Name | Type | Description | Structure | Length |
|---|---|---|---|---|
| BBa_K3040005 | Composite | pFadBA promoter with consensus sequence regulating downstream RFP | pFadBA_NTHU- RBS-mRFP | 953 bp |
| BBa_K3040006 | Composite | pFadBA promoter with mutant consensus sequence regulating downstream RFP | pFadBA_NTHU mutant- RBS -mRFP | 953 bp |
| BBa_K3040007 | Composite | TesA with pFadBA promoter regulating downstream RFP | placUV5-RBS-TesA_pFadBA- RBS -mRFP | 202 bp |
| BBa_K3040008 | Composite | rrnD promoter with TP24 UP element and a fadR binding site inserted before spacer region regulating downstream RFP | TP24-rrnD-fadR binding site-1- RBS -mRFP | 916 bp |
| BBa_K3040009 | Composite | rrnD promoter with TP24 UP element and a fadR binding site inserted between spacer region regulating downstream RFP | TP24-rrnD-fadR binding site-2- RBS -mRFP | 903 bp |
| BBa_K3040010 | Composite | rrnD promoter with TP24 UP element and a fadR binding site inserted after spacer region regulating downstream RFP | TP24-rrnD-fadR binding site-3- RBS -mRFP | 902 bp |
| BBa_K3040011 | Composite | pFadBA promoter with a lacI repressor inserted after Pribnow box | pFadBA-Lac-1- RBS -mRFP | 953 bp |
| BBa_K3040012 | Composite | pFadBA promoter with a lacI repressor inserted after Pribnow box and a FadR repressor inserted before Pribnow box. | pFadBA-Lac-2- RBS -mRFP | 953 bp |
| BBa_K3040013 | Composite | Native pFadD promoter regulating downstream RFP | pFadD- RBS -mRFP | 953 bp |
| BBa_K3040014 | Composite | pFadD promoter with Lac regulating downstream RFP | pFadD_Lac- RBS -mRFP | 106 bp |
| BBa_K3040015 | Composite | pFadD promoter with one fadR binding site regulating downstream RFP | pFadD_fadR binding site- RBS -mRFP | 103 bp |
| BBa_K3040105 | Basic | pFadBA promoter with consensus sequence | pFadBA_NTHU | 184 bp |
| BBa_K3040106 | Basic | pFadBA promoter with mutant consensus sequence | pFadBA_NTHU mutant | 184 bp |
| BBa_K3040107 | Basic | placUV promoter with TesA | placUV promoter with TesA | 896 bp |
| BBa_K3040108 | Basic | rrnD promoter with TP24 UP element and a fadR binding site inserted before spacer region | TP24-rrnD-fadR binding site-1 | 137 bp |
| BBa_K3040109 | Basic | rrnD promoter with TP24 UP element and a fadR binding site inserted between spacer region | TP24-rrnD-fadR binding site-2 | 121 bp |
| BBa_K3040110 | Basic | rrnD promoter with TP24 UP element and a fadR binding site inserted after spacer region | TP24-rrnD-fadR binding site-3 | 120 bp |
| BBa_K3040111 | Basic | One FadR recognition site with pLac promoter | pFadBA-Lac-1 | 201 bp |
| BBa_K3040112 | Basic | Two FadR recognition sites with pLac promoter | pFadBA-Lac-2 | 198 bp |
| BBa_K3040113 | Basic | Native pFadD promoter | pFadD | 297 bp |
| BBa_K3040114 | Basic | pFadD promoter with LacI repressor | pFadD_pLac | 299 bp |
| BBa_K3040115 | Basic | pFadD promoter with one fadR binding site | pFadD_fadR binding site | 255 bp |
Fatty Acid Metabolism Related Parts
| Name | Type | Description | Structure | Length |
|---|---|---|---|---|
| BBa_K3040016 | Composite | Expression of FadR | promotor-RBS -fadR | - |
| BBa_K3040017 | Composite | Expression of FadL | promotor - RBS -fadL | - |
| BBa_K3040018 | Composite | Expression of FadD | promotor - RBS -fadD | - |
| BBa_K3040116 | Basic | FadR | fadR | 1096 bp |
| BBa_K3040117 | Basic | FadL | fadL | 1467 bp |
| BBa_K3040118 | Basic | FadD | fadD | 1786 bp |
Nutrition Related Parts
| Name | Type | Description | Structure | Length |
|---|---|---|---|---|
| BBa_K3040019 | Composite | Expression of urease | promotor - RBS -ureaABC | - |
| BBa_K3040119 | Basic | The union of urease | urease | 2829 bp |
References
- ZHANG, Jin-wei; ZENG, Run-ying. Molecular cloning and expression of a cold-adapted lipase gene from an Antarctic deep sea psychrotrophic bacterium Pseudomonas sp. 7323. Marine biotechnology, 2008, 10.5: 612-621.
- J Microbiol Biotechnol. 2007 Apr;17(4):604-10. Cloning, expression, and characterization of a cold-adapted lipase gene from an Antarctic deep-sea psychrotrophic bacterium, Psychrobacter sp 7195.
- JENSEN, Peter Ruhdal; HAMMER, Karin. The sequence of spacers between the consensus sequences modulates the strength of prokaryotic promoters. Appl. Environ. Microbiol., 1998, 64.1: 82-87.
- ZHANG, Fuzhong; CAROTHERS, James M.; KEASLING, Jay D. Design of a dynamic sensor-regulator system for production of chemicals and fuels derived from fatty acids. Nature biotechnology, 2012, 30.4: 354.
- PRESNELL, Kristin V.; FLEXER-HARRISON, Madeleine; ALPER, Hal S. Design and synthesis of synthetic UP elements for modulation of gene expression in Escherichia coli. Synthetic and systems biotechnology, 2019, 4.2: 99-106.
- ZHANG, Fuzhong; CAROTHERS, James M.; KEASLING, Jay D. Design of a dynamic sensor-regulator system for production of chemicals and fuels derived from fatty acids. Nature biotechnology, 2012, 30.4: 354.
- FENG, Youjun; CRONAN, John E. Crosstalk of Escherichia coli FadR with global regulators in expression of fatty acid transport genes. PloS one, 2012, 7.9: e46275.
- Cover Photo by Caleb Woods on Unsplash
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