Part Collection

Overview

Along with the catalytic activity changes of lipaseA at different temperatures, we can utilize the characteristic of this promoter to establish a dynamic expression system controlled by temperature since different concentration of LCFA can induce different extent of gene expression. However, other iGEM teams such as NTU_Taida 2014 encountered a problem with this promoter. Since pFadBA has a massive leakage, the baseline expression of the reporter protein coupled with the promoter showed no difference when induced with LCFA-coA. This makes the promoter becomes a poor sensor of fatty acid. NTU_Taida 2014 teams solved this problem with the overexpression of fadR protein. Nevertheless, fadR overexpression is toxic to the cell since the normal fatty acid metabolism pathway will have interfered. Hence, we engineered the promoter with many special designs by editing sequence. The fatty acid inducing effect is still retained, but with lower leakage and high fold change.