Team:MADRID UCM/Attributions

Attributions-Bueno – iGem Madrid

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ATTRIBUTIONS

1 Wet Lab

Laura Sáchez Lara
Responsible for the synthetic-biology part of the project. She designed and adapted both the basic and composed DNA parts of the Golden Gate Assembly. She was also in charge of carrying out the protocols and corresponding experiments.
Victor de Lorenzo
From the CNB. He lent his working space and resources to the team through the whole summer period. He also consulted on the process of designing and choosing the system in which we expressed the SynBio.
Juan Nogales
From the CNB. He shared materials for building the MoClo with us. He also helped with the theoretical development of that protocol.
Tomas Aparicio
From the CNB. He instructed the team and helped to perform experiments for characterising the composed constructs of DNA.
Sofía Fraile de la Paz
From the CNB. She worked together with the team throughout the summer, teaching members technical skills and collaborating on the experiments.
Igor Martínez Sánchez and Alejandro Ronco
From the CNB. Their contribution to the lab work consisted of providing explanations and answering queries about the theoretical basis of the MoClo system.
Alejandro Vignoni
Kindly sent us vectors and parts used in the project. Thanks to his guidance, we were able to develop and insert the type enzymes into the competition.
Mercedes Echaide Torreguitar
PI of the team. She lent materials to the team, and allowed a constant flow of the exchange of ideas about the experiments and results obtained throughout the summer.
Paolo Natale
From the Universidad Complutense de Madrid. He helped in the development of the bacteria-transformation experiments.
Francisco Javier Quero
Team leader. He participated in the part design and adaptation for the Golden Gate Assembly.
Claudia Sofía Troncone and Laura Armero Hernández:
In charge of aptamer automation. They participated in the design and adaptation of the automated protocols for the OT-2. They also participated in the corresponding experiments and their manual and automatic demonstration.
Victor González
From the Ramon y Cajal Institute. He supported the team with his contact network, advice and constant availability throughout the summer period. He also helped with the experiments and protocols. Together with his corporate partners, he sponsored the team.
Guillermo Fernández Rodríguez
Guillermo Fernández Rodríguez: Responsible for the SELEX characterisation. His tasks involved the design and adaptation of the conventional protocols for cell adherence to plates, the design and automation of cell-ELONA with OT-2 and the qualitative and quantitative characterization of two aptamers with an already-known sequence.
Jorge Fernández Méndez
Team member. He collaborated on the experiments for the tuning-up of the ELONA and on the qualitative and quantitative characterization of two aptamers with an already-known sequence.
Laura Sánchez Lara
Team member. She performed the design of cell-splitting experiments by manual and automatic magnetic beads.
Jorge Fernández Méndez
Jointly responsible for the development of the sensor. He developed the technology for lateral flow analysis and performed experimental trials. He also took part in the final theoretical design of the device.
Guillermo Fernández Rodríguez
Team member. He participated in the lateral flow technology development. He also took part in the shaping of conjugation protocols
Manuela de las Casas Felgueroso
Jointly responsible for the sensor development. She optimized protocols for the development of the lateral flow analysis sensor.
Francisco Javier Quero Lombardero
Team leader. He participated in the design and development of the lateral flow analysis technology.
Rafael Alejandro Alonso
From the Universidad Complutense de Madrid. He helped with the experiments for the sensor development.
Irene Guardiola Luna and Pablo Villalobos Sánchez
Team members. They carried out the experiments for demonstration of the potentiostat functioning.
Susan Campuzano
From the Universidad Complutense de Madrid. She lent the team materials for making readings with the potentiostat. She also provided the theoretical base for the experiments

2 Dry Lab

Ana Matesanz Fernández-Arias
Responsible for modelling. She designed the mathematical model that predicts the aptamer-folding structure.
Alfonso Rodríguez Patón
From the Polytechnic University of Madrid. He guided and advised on the development of the mathematical model for aptamer folding.
Elena Nuñez Berrueco
From the Polytechnic University of Madrid. She participated in the development of the model.
Irene Guardiola Luna
Team member. She designed the electronics and 3D structures of the OT-2 modules, together with the required plan for their reproducibility.
Francisco Javier Quero Lombardero
Team leader. He designed the electronics and 3D structures of the printed OT-2 modules.
Luis Ángel Tejedor Alvarez
From the Universidad Complutense de Madrid. He provided the work station for the hardware building. He also offered valuable advice when it was most needed.
Carlos Bilbao Muñoz and Pablo Villalobos Sánchez
Responsible for the software part of the project. They were involved in writing all of the team’s code lines.
Ana Matesanz Fernández- Arias
Team member. She designed and programmed the aptamer creation and folding software for the database.

3 Human Practice

Paula Palanco López
Responsible for the human practices. She performed applied ethnographic research on the ground, carrying out interviews and analyzing archival materials. She also collaborated on the organization and logistics of the fieldwork trip to Cameroon.
Thomas Hervé Mboa Nkoudou
From the African Institute of Open Science and Hardware. He helped with the logistics of the fieldwork trip to Cameroon. He let the team use his workspace (MboaLab) and materials for running workshops and experiments on the ground.
Lenshina Agbor
From the African Institute of Open Science and Hardware. She helped with the team’s fieldwork, participating in the workshops and providing further feedback
Stéphane Fadanka
From the African Institute of Open Science and Hardware. He collaborated with the team’s fieldwork, participating in the workshops and providing further feedback.
Jorge Fernández Méndez
Team member. He carried out the necessary tests for the workshop experiments in Cameroon. He also participated in testing the current cholera-detection methodologies.
Francisco Javier Quero Lombardero
Team leader. He organized the logistics and financing of the fieldwork trip to Cameroon. He also took part in the design and running of the MboaLab workshops.
Andrea Palanco López
Team designer. She documented the trip and helped in local-association projects in Cameroon.
Domingo Marquina
From the Universidad Complutense de Madrid. He lent the team space to test the techniques for the Africa workshops and provided the materials used in those workshops.
Laura Herrero de León and Silvia Herrero de León
From the Carlos III Institute. They lent the team their work space for carrying out experiments prior to the fieldwork trip to Cameroon.
Francisco Javier Quero Lombardero
Team leader. He promoted the creation of the High School Madrid_UCM iGEM team. He also organized a number of activities to help with the participation of the High School team in the iGEM competition.
Mercedes Echaide Torreguitar
Team PI. She promoted the creation of the High School Madrid_UCM iGEM team and participated as the PI of both teams, ensuring their mutual collaboration.
Cristina Sánchez García
Vice-rector of investigation of the Universidad Complutense de Madrid. She was responsible for locating working spaces and managing university matters for both Madrid_UCM iGEM teams.
Francisco Javier Quero Lombardero and Jorge Fernández Méndez
Team members. They were in charge of carrying out the safety form.

4 Graphic design

Andrea Palanco López
team designer. She was responsible for the whole graphic design process of the project. She carried out the visual and creative tasks and created the corporate identity of the project and the team.

5 Wiki

Andrea Palanco López
team designer. She created the web design and designed the graphic content.
Pablo Villalobos Sanchez, Carlos Bilbao Muñoz and Francisco Javier Quero
Team members. They programmed and uploaded all the contents, graphics and effects of the wiki page.
Paula Palanco López
Revised and edited the written contents included in the wiki.
Charlie Bowen
External proofreader. Proofread the written contents included in the wiki.
Team Madrid_UCM
Wrote the contents included in the wiki.

6 Organization

Francisco Javier Quero Lombardero
Team leader. He sourced and contacted the team sponsors.
Cristina Sánchez García
Vice-rector of investigation of the Universidad Complutense de Madrid. She helped with funding and management within the Universidad Complutense de Madrid.
Manuela de las Casas Felgueroso, Paula Palanco López and Andrea Palanco López
Team members. They took charge of the team’s social network activities.
Francisco Javier Quero Lombardero
Team leader. He organized and guided the project and team members throughout the year.
Martín Gúrpide
Director of MK press. He actively participated in the project’s exposure by releasing a number of press notes through several media throughout the year.
Mª Teresa Villalba Díaz
From the Universidad Complutense de Madrid. She promoted the project by organizing various seminars in the Chemistry Faculty, and participated together with the team on the radio program “Hombros de Gigantes” (RTVE).
Cristina Sánchez García
Vice-rector of investigation of the Universidad Complutense de Madrid. She promoted several exposure events within the university and boosted the presence of the team in the university media.
Francisco Javier Quero Lombardero
Team leader. He contacted a number of sources and promoted the team’s exposure
Paula Palanco López
Team member. She helped with the networking and exposure process.