Team:Gunma/Results

Team:Gunma Results

Results



Characterization of BBa_I746909 : Succeeded!!


100 μM IPTG added BL21(DE3) transformed pSB1C3, BBa_I746909


Comparison between IPTG added and not


800μM IPTG added culture fluid of BL21(DE3) transformed pSB1C3,BBa_I746909


800μM IPTG added culture fluid of BL21(DE3) transformed pSB1C3,BBa_I746909



Preliminary Experiments : Succeeded!!


(1)

(2)



Main Experiments : Validation Succeeded!!


Fig.1 BL21(DE3) pSB1C3 BBa_K3050000 expressed under visible light with negative control without IPTG induction

Fig.2 pSB1C3 BBa_K3050000 expressed under UV light with negative control without IPTG induction

Fig.3 Four biological replicates all highly expressed

Fig.4 500 μM IPTG induced BBa K3050000, strongly emitting green fluorescent light

NOTE : BBa_K3050001 is validated as a part of BBa_K3050000.