Team:Gunma/Results
Results
Characterization of BBa_I746909 : Succeeded!!
100 μM IPTG added BL21(DE3) transformed pSB1C3, BBa_I746909
Comparison between IPTG added and not
800μM IPTG added culture fluid of BL21(DE3) transformed pSB1C3,BBa_I746909
800μM IPTG added culture fluid of BL21(DE3) transformed pSB1C3,BBa_I746909
Preliminary Experiments : Succeeded!!
(1)
(2)
Main Experiments : Validation Succeeded!!
Fig.1 BL21(DE3) pSB1C3 BBa_K3050000 expressed under visible light with negative control without IPTG induction
Fig.2 pSB1C3 BBa_K3050000 expressed under UV light with negative control without IPTG induction
Fig.3 Four biological replicates all highly expressed
Fig.4 500 μM IPTG induced BBa K3050000, strongly emitting green fluorescent light
NOTE : BBa_K3050001 is validated as a part of BBa_K3050000.
