Our Initial Project Design
Generation 1 RED
Generation 2 BLUE
Generation 3 GREEN
Generation 4 DEATH!!
We were inspired by the project of team UT-Tokyo 2016, and planned to improve their project by adding our original generation that expresses self-killing system. However we decided to focus on building our original "DEATH" section after all.
Our newest design is shown below.
1.Confirm BL21(DE3)pLysS tend to lyse in hypotonic solution, but the wild E.coli don't.
2.Confirm that T7 RNA polymerase can inhibit amidase activity of T7 lysozyme besically expressed in BL21(DE3)pLysS.
3.Build a system that self restrain proliferation of BL21(DE3)pLysS cultured in a solution with T7 RNA polymerase.
4.Build a part that express toxic protein weaken cell membrane.
5.Transform the part above into BL21(DE3)pLysS to make it lyse more easily than BL21(DE3)pLysS.
Designed by team UT-Tokyo 2016 cited byhttp://2016.igem.org/Team:UT-Tokyo/Project#system
What is our future vision?
Currently we are required many facilities to treat GMO or conducting DNA recombinant experiments for Biosafety. For example we need to implement all the experiments in Lab with proper facility, in negative pressure, outside shoes strictly prohibited and so on, or in cleanbench, and use autioclave to make sure to kill all the GMO we made not to release them outside. These facilities are essential but expensive. Hence it is sometimes cause economical unfairness between economically rich countries and relatively poor countries.
Our idea is still supporting the current rules but also aim to add another cheao means to avoid biohazard: building a system inside microorganism that regulates its life in advance.