Team:GZHS-United/Improve

• Team
• Project
• Parts
• Safety
• Human Practices
• Awards

• Team Members
• Collaborations

• Description
• Design
• Experiments
• Notebook
• Contribution
• Results
• Demonstrate
• Improve
• Attributions

• Parts Overview
• Basic Part
• Composite Part
• Part Collection

• Human Practices
• Education & Engagement

• Model

Improve

Part:BBa-K2948003

GroE promoter

The default state of this promoter is off and can be induced by heat. It is 44 base pairs long and is located 72 bases upstream of the start site of groE. The groE gene is from E. coli.

BBa-K2948005

Heme Oxygenase with GroE & RBS (E. coli Codon Optimised)

Hemeoxygenase with RBS (E. coli Codon Optimised), sequences obtained from BBa_K953000, and we turned the T7 promoter(Part BBa_I712074) into GroE promoter (BBa_K763001) This complex can then absorb red light (620-750 nm) to excite the bacteriophytochrome and result in a phenotypic change from blue to green. This can be reversed by far-red light (700-800 nm) or will revert from green to blue over time. As E. coli does not produce biliverdin, heme oxygenase must be coupled with a bacteriophytochrome to activate the oxidation of heme to produce biliverdin.

We changed the sequence of heme device in BBa K953000. We replace the T7 promoter with the promoter GroE. By taking photos and comparing the effects of different induction temperatures of plasmid pGroE-heme on the expression of target protein, we explored the expression induced by promoter GroE at different induction temperatures.

© 2019 Team:GZHS-United IGEM

arrow_upward