Part:BBa-K2948006
Part:BBa-ATAPX1 with PrplJ
ATAPX1 (BBa_K2948004) encodes a cytosolic ascorbate peroxidase APX1. Ascorbate peroxidases are enzymes that scavenge hydrogen peroxide in plant cells.
PrplJ (BBa_K2559003) is E. coli promoter of sgRNA
We put it into the escherichia coli culture. We made e. coli express the protein and test its enzyme activity at different ph, temperature and light conditions.
Here is the result:
Our part is also in accordance with the igem competition official.