In this project, we designed and built 28 new biological parts includes 13 basic parts, 14 composite parts and 1 device.
Here is the total list. You can get more information on the pages of Basic Part, Composite Part and Part Collection.
Part Name |
Full Name |
Nickname |
Type |
Note |
phiC31attB-ECK120034435-phiC31attPr |
IBR-C35 |
Composite |
the sequence between att sites will be FLIPPED |
|
Int5attP-ECK120010855-Int5attB |
IBR-F55 |
Composite |
the sequence between att sites will be EXCISED |
|
Int7attB-ECK120033737-Int7attP |
IBR-S37 |
Composite |
the sequence between att sites will be EXCISED |
|
Int8attB-ECK120030221-Int8attP |
IBR-E21 |
Composite |
the sequence between att sites will be EXCISED |
|
Int10attB-ECK120020525-Int10attP |
IBR-T25 |
Composite |
the sequence between att sites will be EXCISED |
|
TG1attB-L3S3P22-TG1attP |
IBR-G22 |
Composite |
the sequence between att sites will be EXCISED; |
|
Int5 integrase |
INT5 |
CDS |
||
Int7 integrase |
INT7 |
CDS |
||
Int8 integrase |
INT8 |
CDS |
||
Int10 integrase |
INT10 |
CDS |
||
Translational Unit for phiC31 integrase |
phiC31TLU1 |
Translational Unit |
the RBS is same to the phiC31 phage genome, so is the start codon |
|
Translational Unit for Int8 integrase |
INT8TLU1 |
Translational Unit |
||
Translational Unit for Int10 integrase |
INT10TLU1 |
Translational Unit |
||
Translational Unit for TG1 integrase |
TG1TLU1 |
Translational Unit |
the RBS is same to the TG1 phage genome. |
|
Optimized Ptac promoter |
PtacOpt |
Promoter |
A twin of BBa_K2572025 |
|
Mutant Ptac promoter No.1 |
Ptac-M1 |
Promoter |
||
Mutant Ptac promoter No.2 |
Ptac-M2 |
Promoter |
||
Mutant Ptac promoter No.3 |
Ptac-M3 |
Promoter |
||
original sequence of phiC31 integrase gene |
OriphiC31 |
Translational Unit |
||
A RBS used in BBa_K3254011 |
int8RBS1 |
RBS |
||
A RBS used in BBa_K3254012 |
int10RBS1 |
RBS |
||
A RBS used in BBa_K3254013 |
TG1RBS1 |
RBS |
||
lacI constitutive expression cassette |
lacI-CEC |
Expressional Unit |
||
RiboJ-B0034-sfGFP |
sfGFPTLU |
Translational Unit |
||
Ptac-RiboJ-sfGFP |
Ptac-sfGFP |
Operon |
||
Translational Unit for Int5 integrase |
INT5TLU1 |
Translational Unit |
||
Translational Unit for Int7 integrase |
INT7TLU1 |
Translational Unit |
||
A RBS used in BBa_K3254026 |
int5RBS1 |
RBS |
||
A RBS used in BBa_K3254027 |
in7RBS1 |
RBS |