Gold Medal!

We built a thermodynamics model under equilibrium state to describe quantitative relationship between the concentration of recombinase and the recombination efficiency. The parameters we employed are corresponding to physical meanings. We used this model to simulate the result of the experiment and successfully proof that the specific recombination of recombinase sites attB/attP have a narrow hypersensitive response interval and when the input is greater than a certain threshold, the output of recombinase will dramatically increase, consequently proofing the feasibility of our design. The result of the experiment fit the prediction of the model well. Furthermore, the model can describe the performance of the relay by the parameterization of relay element. This can improve the modularization of the bio-relay, simplify the processing of choosing recombinase.

Measurement

https://2019.igem.org/Team:GENAS_China/Measurement

In order to fit the assumption of that the reaction is under equilibrium, we develop a new precise measurement based on steady state measuring method in order to determine the recombination efficiency accurately. By separating the inducing process of recombinase expression and GFP reporting system, we are able to minimize the strain growth difference due to protein expression burden, improving the accuracy of the experiment. What’s more, we also simplify the steady state measuring, shorten the time of whole measuring process. This new method can get similar result of the conventional method and the accuracy can meet the requirements of characterization of elements.

Software Tool

https://2019.igem.org/Team:GENAS_China/Software

We build our mathematical model based on thermodynamic theories. Our programs, written in R language and MATLAB respectively, can use this model to stimulate experiments and conduct regression analysis on experimental data. Thus, we achieve to describe our components (recombinase-based biological relay) parametrically. Such programs will contribute to the establishment of the parameterized database of the recombinase system and this is a necessary condition to realize the computer-assisted automated designing of the biological circuit.

Best Part Collection

Part Collection page.

From Part BBa_K3254000 to Part BBa_K3254005 are the genetic switches of structure attB-BsaI site-terminator-attP. These genetic switches can response to the expression intensity of the corresponding recombinases, and change the expression states of the downstream gene or the gene between the BsaI cutting sites. From Part BBa_K3254010 to Part BBa_3254013 are the recombinase translational units connected to RiboJ insulator. By collocating the above-mentioned parts, we can create a set of orthogonal recombinase-based biological relay with different response range to the input signal.

Team:GENAS China/Awards

Gold Medal!

Best Model - High school

Other Nominations

Award Requirements

Requirements for Bronze:

1. Registration and Jamboree Attendance: https://igem.org/2019_Judging_Form?team=GENAS_China

2. Competition Deliverables: https://igem.org/2019_Judging_Form?team=GENAS_China

3. Attributions:

4. Project Inspiration and Description:

5. Characterization:

Requirements for Silver:

1. Validated Part/Validated Contribution:

2. Collaboration:

3. Human Practices:

Requirements for Gold:

1. Integrated Human Practices:

2. Improvement of a previous part

3. Model

4. Demonstration

Special prizes:

Model

Measurement

Software Tool

Best Part Collection