We have developed a method to measure hydrogen gas production using novel spectra created using a scanning fluorometer.
H2 detection can be performed using H2Blue, a reagent that is blue but turns clear in the presence of dissolved hydrogen gas; as such, its color can be used to quantitatively assess the presence of dissolved hydrogen in a sample. Using the spectra that we have measured, hydrogen gas can be easily detected and quantified without requiring more complex setups like gas chromatography or a subsequent reaction to find the concentration through stoichiometry. The optimal wavelength for absorbance measurement was found to be 650 nm for the H2Blue. An example of data obtained by using H2 Blue is shown below (note that this is not our experiment's actual data, but just an example).