Difference between revisions of "Team:Marburg/Collaborations"

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                     <div class="content-inner" style="text-align: left;">
 
                     <div class="content-inner" style="text-align: left;">
 
                       <h3>Interlab - Accelerate Golden Gate Assembly</h3>
 
                       <h3>Interlab - Accelerate Golden Gate Assembly</h3>
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                       <figure Style="float:left; margin-left: 25px;">
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                       <figure style="float:left; margin-left: 25px;">
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                         <img style="height: 50ex; width: 40ex"
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                         <img src="  https://static.igem.org/mediawiki/2019/7/79/T--marburg--interlab_button.svg" alt="Interlab button">
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                          src="  https://static.igem.org/mediawiki/2019/7/79/T--marburg--interlab_button.svg"
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                          alt="Interlab button">
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                       </figure>
 
                       </figure>
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                      <br> <br>
 
 
                       Interlab - Accelerate Golden Gate Assembly
 
                       Interlab - Accelerate Golden Gate Assembly
 
                       As we are constantly working on improving the efficiency of Golden Gate. We clearly
 
                       As we are constantly working on improving the efficiency of Golden Gate. We clearly
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                       cycler settings for different applications.
 
                       cycler settings for different applications.
 
                       Although these threeprotocols were tested before, they were never compared to each other in
 
                       Although these threeprotocols were tested before, they were never compared to each other in
−
                       their
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                       their efficiency.<br>
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                      efficiency.<br>
+
 
                       We conducted our own Interlab study in collaboration with the iGEM Teams from
 
                       We conducted our own Interlab study in collaboration with the iGEM Teams from
 
                       <a href="https://2019.igem.org/Team:TUDelft"> TUDelft </a>,
 
                       <a href="https://2019.igem.org/Team:TUDelft"> TUDelft </a>,
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                             <td style="text-align: center"> <a href="link 7"> Kan-RFP BBa_K2560056 </a>
 
                             <td style="text-align: center"> <a href="link 7"> Kan-RFP BBa_K2560056 </a>
 
                           </tr>
 
                           </tr>
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+
                     
 
                         </tbody>
 
                         </tbody>
 
                       </table>
 
                       </table>
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                       <br> <br>
 
                       <br> <br>
 
                       <figure Style="text-align:center">
 
                       <figure Style="text-align:center">
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                         <img style="height: 60ex; width:80ex"
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                         <img style="height: 60ex; width:80ex" src="https://static.igem.org/mediawiki/2019/9/91/T--marburg--interlab_package.jpeg"
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                          src="https://static.igem.org/mediawiki/2019/9/91/T--marburg--interlab_package.jpeg"
+
 
                           alt="Interlab oackage">
 
                           alt="Interlab oackage">
 
                         <figcaption>
 
                         <figcaption>
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                       <br>
 
                       <br>
 
                       The handout with the used protocols can be downloaded <a
 
                       The handout with the used protocols can be downloaded <a
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                         href=https://static.igem.org/mediawiki/2019/a/a0/T--marburg--Interlab_protocoll.pdf> here </a> </div>
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                         href=https://static.igem.org/mediawiki/2019/a/a0/T--marburg--Interlab_protocoll.pdf>here </a>
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                        </div> </div> </section> <div class="sub" onclick="popup('rbn3')">
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                        <div class="sub-header">
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                          <h1>
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                            B E R L I N / D E L F T
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                          </h1>
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                          <hr>
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                        </div>
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                        <div class="sub-content">
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                          <p>
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                          </p>
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                     </div>
 
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                </div>
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                        <div class="popup-header">
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              </section>
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                          <h1 class="title">Berlin/Delft Collaborations</h1>
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              <div class="sub" onclick="popup('rbn3')">
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                          <button type="button" onclick="hide('rbn3')">X</button>
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                <div class="sub-header">
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                  <h1>
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                        <div class="popup-content" style="text-align: justify;">
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                    B E R L I N / D E L F T
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                          <div>
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                  </h1>
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                            <p>
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                  <hr>
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                              <figure Style="float:right; margin-left: 25px;">
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                </div>
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                                <img style="height: 15ex; width: 35ex"
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                <div class="sub-content">
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                                  src="  https://static.igem.org/mediawiki/2019/9/9b/T--marburg--berlin_logo_richtig.png"
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                  <p>
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                                  alt="Berlin logo">
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                              </figure>
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                              iGEM Berlin engineered the freshwater algae Chlamydomonas reinhardtii to degrade
+
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                              Polyethylene
+
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                              terephthalate
+
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                              (PET). For this goal they also built a bioreactor for their chassis. We sent
+
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                              them our UTEX
+
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                              strain to test if
+
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                              their photobioreactor is capable to cultivate other phototrophic organisms as
+
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                              well. They gained
+
−
                              additional
+
−
                              experiences on growth conditions for phototrophic organisms and we could see our
+
−
                              strains grow in
+
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                              a
+
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                              bioreactor environment.
+
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                              <br>
+
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                              Click here to learn more about there project <a
+
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                                href="https://2019.igem.org/Team:Humboldt_Berlin"> Chlamylicious </a>
+
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                              <br>
+
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                              <br>
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                              <br> <br> <br>
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                              <h> <b>Delft Collaborations </b></h> <!-- Delf-->
+
  
 +
                  </p>
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                </div>
 +
              </div>
 +
              <div id="rbn3" class="popup">
 +
                <div class="popup-container">
 +
                  <div class="popup-header">
 +
                    <h1 class="title">Berlin/Delft Collaborations</h1>
 +
                    <button type="button" onclick="hide('rbn3')">X</button>
 +
                  </div>
 +
                  <div class="popup-content" style="text-align: justify;">
 +
                    <div>
 +
                      <p>
 +
                        <figure Style="float:right; margin-left: 25px;">
 +
                          <img style="height: 15ex; width: 35ex"
 +
                            src="  https://static.igem.org/mediawiki/2019/9/9b/T--marburg--berlin_logo_richtig.png"
 +
                            alt="Berlin logo">
 +
                        </figure>
 +
                        iGEM Berlin engineered the freshwater algae Chlamydomonas reinhardtii to degrade
 +
                        Polyethylene
 +
                        terephthalate
 +
                        (PET). For this goal they also built a bioreactor for their chassis. We sent
 +
                        them our UTEX
 +
                        strain to test if
 +
                        their photobioreactor is capable to cultivate other phototrophic organisms as
 +
                        well. They gained
 +
                        additional
 +
                        experiences on growth conditions for phototrophic organisms and we could see our
 +
                        strains grow in
 +
                        a
 +
                        bioreactor environment.
 +
                        <br>
 +
                        Click here to learn more about there project <a
 +
                          href="https://2019.igem.org/Team:Humboldt_Berlin"> Chlamylicious </a>
 +
                        <br>
 +
                        <br>
 +
                        <br> <br> <br>
 +
                        <h> <b>Delft Collaborations </b></h> <!-- Delf-->
  
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                              <figure Style="float:left; margin-right: 25px;">
 
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                                <img style="height: 60 ex; width: 40ex"
 
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                                  src="https://static.igem.org/mediawiki/2019/5/5e/T--marburg--Skype_DELF.jpeg"
 
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                                  alt=" DELFT Bild">
 
−
                                <figcaption> Fig. 1: Skype talk with TU Delft</figcaption>
 
−
                              </figure>
 
  
−
                              <figure Style="float:right; margin-left: 25px;">
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                        <figure Style="float:left; margin-right: 25px;">
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                                <img style="height: 30ex; width: 30ex"
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                          <img style="height: 60 ex; width: 40ex"
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                                  src="https://static.igem.org/mediawiki/2019/d/d4/T--marburg--DELF_logo_richtig.svg"
+
                            src="https://static.igem.org/mediawiki/2019/5/5e/T--marburg--Skype_DELF.jpeg" alt=" DELFT Bild">
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                                  alt="DELF logo">
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                          <figcaption> Fig. 1: Skype talk with TU Delft</figcaption>
 +
                        </figure>
  
−
                              </figure>
+
                        <figure Style="float:right; margin-left: 25px;">
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                              <br>
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                          <img style="height: 30ex; width: 30ex"
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                              iGEM Delft created an all species encompassing toolbox, based on orthogonal gene
+
                            src="https://static.igem.org/mediawiki/2019/d/d4/T--marburg--DELF_logo_richtig.svg"
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                              expression and
+
                            alt="DELF logo">
−
                              host
+
−
                              independent plasmid replication. We exchanged multiple times over Molecular
+
−
                              Cloning and toolbox
+
−
                              designs.
+
−
                              Additionally, we sent them parts in the PhytoBricks standard.
+
  
−
                              Click here to learn more about there project <a href="https://2019.igem.org/Team:TUDelft">
+
                        </figure>
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                                project SCI-PHI 29 </a>
+
                        <br>
 +
                        iGEM Delft created an all species encompassing toolbox, based on orthogonal gene
 +
                        expression and
 +
                        host
 +
                        independent plasmid replication. We exchanged multiple times over Molecular
 +
                        Cloning and toolbox
 +
                        designs.
 +
                        Additionally, we sent them parts in the PhytoBricks standard.
  
−
                              <br>
+
                        Click here to learn more about there project <a href="https://2019.igem.org/Team:TUDelft">
−
                            </p>
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                           project SCI-PHI 29 </a>
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                           </div>
+
 
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                          <br>
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                        <br>
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                          <br>
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                       </p>
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                          <br>
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                        </div>
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                       </div>
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                     </div>
 
                     </div>
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                     <div class="sub" onclick="popup('rbn4')">
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                     <br>
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                      <!--UC Davis pop up-->
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                    <br>
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                      <div class="sub-header">
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                    <br>
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                        <h1>
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                  </div>
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                          U C<span style="visibility: hidden;">A</span>D A V I S
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                </div>
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                        </h1>
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              </div>
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                        <hr>
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              <div class="sub" onclick="popup('rbn4')">
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                      </div>
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                <!--UC Davis pop up-->
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                        <p>
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                  <h1>
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                    U C<span style="visibility: hidden;">A</span>D A V I S
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                  </h1>
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                  <hr>
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                </div>
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                <div class="sub-content">
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                  <p>
  
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              <div id="rbn4" class="popup">
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                <div class="popup-container">
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                  <div class="popup-header">
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                    <h1 class="title">UC Davis collaboration</h1>
 +
                    <button type="button" onclick="hide('rbn4')">X</button>
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                  </div>
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                  <div class="popup-content">
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                    <div>
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                      <p>
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                         <figure Style="float:left; margin-right: 25px;">
 +
                          <img style="height: 50ex; width: 40ex"
 +
                            src="https://static.igem.org/mediawiki/2019/4/49/T--marburg--UCD_OT2.jpeg" alt="UCD OT2">
 +
                        </figure>
 +
                        UC Davis iGEM team is one of the winners of the Opentrons competition in 2019.
 +
                        iGEM Marburg
 +
                        won one OT-2
 +
                        during last
 +
                        year’s edition of the competition and got contacted for a collaboration by iGEM
 +
                        UC Davis
 +
                        along with other
 +
                        winners from
 +
                        2018 and 2019.
 +
                        The goal for this automation InterLab collaboration was to collect data to
 +
                        determine the
 +
                        accuracy and
 +
                        reproducibility of
 +
                        the OT-2 compared to humans. To do this, UC Davis proposed to perform the
 +
                        standard iGEM
 +
                        protocol on serial
 +
                        dilutions
 +
                        with silica beads and fluorescein, each manually and with an OT-2. In total iGEM
 +
                        Marburg did
 +
                        four dilutions;
 +
                        twice with
 +
                        an OT-2 and twice manually. The data was then sent to UC Davis via the
 +
                        spreadsheet designed
 +
                        by them.
 +
                        Finally, iGEM
 +
                        Marburg aided by creating a Slack channel for all iGEM teams with an OT-2 to
 +
                        facilitate
 +
                        better communication
 +
                        and
 +
                        exchange in the future.
 +
                        <br><br>
 +
                        In total, seven teams participated and sent their data to UC Davis which are
 +
                        shown in the
 +
                        figure below.
 +
                        According to UC
 +
                        Davis, the fluorescence data was standardized using the iGEM MEFL conversion to
 +
                        standardize
 +
                        the plate reader
 +
                        measurements. Subsequently, the data was fitted using a linear regression model,
 +
                        whose
 +
                        quality is quantified
 +
                        using the
 +
                        r2 metric (it ranges from 0 to 1 with higher value indicates a better fit).
 +
                        <br>
 +
                        For the fluorescence the r2 value from the OT2 data beats human r2 value with
 +
                        0.94 and 0.90,
 +
                        respectively.
 +
                        Interestingly, for the silica beads, manual measurements yielded a more
 +
                        satisfactory result
 +
                        with r2 value of
 +
                        0.90
 +
                        compared to 0.82 from OT2 measurements. One possible explanation could be the
 +
                        speed of the
 +
                        OT-2: it took
 +
                        twice as long
 +
                        to complete this task with the OT-2 compared to a manual trial. This could lead
 +
                        to the
 +
                        sedimentation of the
 +
                        silica beads
 +
                        as the OT-2 went through their runs; therefore worsening its results. Also, as
 +
                        shown in Fig.
 +
                        2b, one team’s
 +
                        result
 +
                        deviates a lot from the rest of the teams, maybe if their result is taken from
 +
                        the overall
 +
                        evaluation a more
 +
                        comparable
 +
                        result between OT-2 and human can be observed.
 +
                        <br>
 +
                        <br>
 +
                        <figure Style="text-align:center">
 +
                          <img style="height: 50ex; width: 50ex"
 +
                            src="https://static.igem.org/mediawiki/2019/c/cc/T--marburg--UC_Davis_Graphic_1.png"
 +
                            alt=" UCD Graphic 1">
 +
                          <img style="height: 50ex; width: 50ex"
 +
                            src=" https://static.igem.org/mediawiki/2019/a/a7/T--marburg--UC_Davis_Graphic_2.png"
 +
                            alt=" UC Davis Graphic 2">
 +
                          <figcaption1>
 +
                            <br> Figure 1: a)OT-2 vs manual fluorescein measurement experiment. OT-2
 +
                            performed better
 +
                            with r2 value of 0.94 compared to 0.90 of human. b)OT-2 vs manual silica
 +
                            beads measurement experiment. Human performed better
 +
                            with r2 value of 0.90 compared to 0.82 of OT-2.
 +
                            </figcaption>
 +
                        </figure>
 +
                        <br>
 +
                      </p>
 +
                       <br>More information about this collaboration such as individual team data can be
 +
                      found on UC
 +
                      Davis wiki:
 +
                      <a href="https://2019.igem.org/Team:UC_Davis/Collaborations"> Wiki UC Davis
 +
                        Collaboration </a>
 +
                      <br>
 +
                      </p>
 
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                          <h1 class="title">UC Davis collaboration</h1>
 
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                              <figure Style="float:left; margin-right: 25px;">
 
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                                <img style="height: 50ex; width: 40ex"
 
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                                  src="https://static.igem.org/mediawiki/2019/4/49/T--marburg--UCD_OT2.jpeg" alt="UCD OT2">
 
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                              </figure>
 
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                              UC Davis iGEM team is one of the winners of the Opentrons competition in 2019.
 
−
                              iGEM Marburg
 
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                              won one OT-2
 
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                              during last
 
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                              year’s edition of the competition and got contacted for a collaboration by iGEM
 
−
                              UC Davis
 
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                              along with other
 
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                              winners from
 
−
                              2018 and 2019.
 
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                              The goal for this automation InterLab collaboration was to collect data to
 
−
                              determine the
 
−
                              accuracy and
 
−
                              reproducibility of
 
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                              the OT-2 compared to humans. To do this, UC Davis proposed to perform the
 
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                              standard iGEM
 
−
                              protocol on serial
 
−
                              dilutions
 
−
                              with silica beads and fluorescein, each manually and with an OT-2. In total iGEM
 
−
                              Marburg did
 
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                              four dilutions;
 
−
                              twice with
 
−
                              an OT-2 and twice manually. The data was then sent to UC Davis via the
 
−
                              spreadsheet designed
 
−
                              by them.
 
−
                              Finally, iGEM
 
−
                              Marburg aided by creating a Slack channel for all iGEM teams with an OT-2 to
 
−
                              facilitate
 
−
                              better communication
 
−
                              and
 
−
                              exchange in the future.
 
−
                              <br><br>
 
−
                              In total, seven teams participated and sent their data to UC Davis which are
 
−
                              shown in the
 
−
                              figure below.
 
−
                              According to UC
 
−
                              Davis, the fluorescence data was standardized using the iGEM MEFL conversion to
 
−
                              standardize
 
−
                              the plate reader
 
−
                              measurements. Subsequently, the data was fitted using a linear regression model,
 
−
                              whose
 
−
                              quality is quantified
 
−
                              using the
 
−
                              r2 metric (it ranges from 0 to 1 with higher value indicates a better fit).
 
−
                              <br>
 
−
                              For the fluorescence the r2 value from the OT2 data beats human r2 value with
 
−
                              0.94 and 0.90,
 
−
                              respectively.
 
−
                              Interestingly, for the silica beads, manual measurements yielded a more
 
−
                              satisfactory result
 
−
                              with r2 value of
 
−
                              0.90
 
−
                              compared to 0.82 from OT2 measurements. One possible explanation could be the
 
−
                              speed of the
 
−
                              OT-2: it took
 
−
                              twice as long
 
−
                              to complete this task with the OT-2 compared to a manual trial. This could lead
 
−
                              to the
 
−
                              sedimentation of the
 
−
                              silica beads
 
−
                              as the OT-2 went through their runs; therefore worsening its results. Also, as
 
−
                              shown in Fig.
 
−
                              2b, one team’s
 
−
                              result
 
−
                              deviates a lot from the rest of the teams, maybe if their result is taken from
 
−
                              the overall
 
−
                              evaluation a more
 
−
                              comparable
 
−
                              result between OT-2 and human can be observed.
 
−
                              <br>
 
−
                              <br>
 
−
                              <figure Style="text-align:center">
 
−
                                <img style="height: 50ex; width: 50ex"
 
−
                                  src="https://static.igem.org/mediawiki/2019/c/cc/T--marburg--UC_Davis_Graphic_1.png"
 
−
                                  alt=" UCD Graphic 1">
 
−
                                <img style="height: 50ex; width: 50ex"
 
−
                                  src=" https://static.igem.org/mediawiki/2019/a/a7/T--marburg--UC_Davis_Graphic_2.png"
 
−
                                  alt=" UC Davis Graphic 2">
 
−
                                <figcaption1>
 
−
                                  <br> Figure 1: a)OT-2 vs manual fluorescein measurement experiment. OT-2
 
−
                                  performed better
 
−
                                  with r2 value of 0.94 compared to 0.90 of human. b)OT-2 vs manual silica
 
−
                                  beads measurement experiment. Human performed better
 
−
                                  with r2 value of 0.90 compared to 0.82 of OT-2.
 
−
                                  </figcaption>
 
−
                              </figure>
 
−
                              <br>
 
−
                            </p>
 
−
                            <br>More information about this collaboration such as individual team data can be
 
−
                            found on UC
 
−
                            Davis wiki:
 
−
                            <a href="https://2019.igem.org/Team:UC_Davis/Collaborations"> Wiki UC Davis
 
−
                              Collaboration </a>
 
−
                            <br>
 
−
                            </p>
 
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                          <br>
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                    <br>
  
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                          U F L O R I D A
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                    U F L O R I D A
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                        </h1>
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                  </h1>
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                          <h1 class="title">UFlorida</h1>
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                    <h1 class="title">UFlorida</h1>
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                          <button type="button" onclick="hide('rbn5')">X</button>
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               <div></div><p>
 
               <div></div><p>
 
                     <br>
 
                     <br>
  
 
                     <figure  Style=" float:left; margin-right: 25px;">
 
                     <figure  Style=" float:left; margin-right: 25px;">
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                          <img style="height: 30ex; width: 40ex"
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                    <img style="height: 30ex; width: 40ex"
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                            src="https://static.igem.org/mediawiki/2019/f/f7/T--Florida--iGEMWeb1.png" alt="Florida">
+
                      src="https://static.igem.org/mediawiki/2019/f/f7/T--Florida--iGEMWeb1.png" alt="Florida">
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                          <figcaption>
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                    </figure>
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                          This semester we had the pleasure of mentoring UFlorida for their Wiki
+
                    This semester we had the pleasure of mentoring UFlorida for their Wiki
−
                          design. Our member, Rene Inckemann, aided UFlorida in their quest to create
+
                    design. Our member, Rene Inckemann, aided UFlorida in their quest to create
−
                          a visually accessible Wiki, similar to what we did this past iGEM season.
+
                    a visually accessible Wiki, similar to what we did this past iGEM season.
−
                          We helped shape UFlorida’s website into an interactive and appealing
+
                    We helped shape UFlorida’s website into an interactive and appealing
−
                          landscape, while maintaining the integrity of their goal and project. We
+
                    landscape, while maintaining the integrity of their goal and project. We
−
                          advised them on their font choices- specifically their use of bold and
+
                    advised them on their font choices- specifically their use of bold and
−
                          italicized words-, along with answering questions about our work with the
+
                    italicized words-, along with answering questions about our work with the
−
                          visually disabled community and utilizing our “Accessible Wiki Guide” from
+
                    visually disabled community and utilizing our “Accessible Wiki Guide” from
−
                          the previous year.
+
                    the previous year.
−
                          <br>
+
                    <br>
−
                          Besides answering questions specifically about their project, we also
+
                    Besides answering questions specifically about their project, we also
−
                          offered valuable information about when to start working on the Wiki and
+
                    offered valuable information about when to start working on the Wiki and
−
                          questions about coding. Working with a young team like UFlorida offered us
+
                    questions about coding. Working with a young team like UFlorida offered us
−
                          a great mentorship opportunity.
+
                    a great mentorship opportunity.
−
                          <br>
+
                    <br>
  
−
                          <figure style="align-content:center;">
+
                    <figure style="align-content:center;">
−
                            <img src="https://static.igem.org/mediawiki/2019/2/29/T--marburg--UFlorida_Rene.jpeg"
+
                      <img src="https://static.igem.org/mediawiki/2019/2/29/T--marburg--UFlorida_Rene.jpeg" alt="Florida">
−
                              alt="Florida">
+
                      <figcaption>
−
                            <figcaption>
+
                        Fig. 1: Skypecall with UFlorida
−
                              Fig. 1: Skypecall with UFlorida
+
                      </figcaption>
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                            </figcaption>
+
                    </figure>
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                          </figure>
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                    <br>
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                          <br>
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                  <h1>I N T E R A C T I O N S</h1>
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                        <h1>I N T E R A C T I O N S</h1>
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                  <h1>W I T H</h1>
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                        <h1>W I T H</h1>
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                  <h1>O T H E R<span style="visibility: hidden;">A</span>T E A M S</h1>
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                        <h1>O T H E R<span style="visibility: hidden;">A</span>T E A M S</h1>
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                  <hr>
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                    <h1 class="title">Interactions with other teams</h1>
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                          <h1 class="title">Interactions with other teams</h1>
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                    <p>
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                          <p>
+
  
−
                            <b>Mike the microbe</b>
+
                      <b>Mike the microbe</b>
−
                            <figure Style="text-align:center">
+
                      <figure Style="text-align:center">
−
                              <img src="  https://static.igem.org/mediawiki/2019/b/b0/T--marburg--Mike_microbe.png"
+
                        <img src="  https://static.igem.org/mediawiki/2019/b/b0/T--marburg--Mike_microbe.png"
−
                                alt=" Mike the Mircrobe">
+
                          alt=" Mike the Mircrobe">
−
                              <figcaption>
+
                        <figcaption>
−
                                Figure 1: Mike the mircrobe in our lab.
+
                          Figure 1: Mike the mircrobe in our lab.
−
                              </figcaption>
+
                        </figcaption>
−
                            </figure>
+
                      </figure>
−
                            We participated in the “Mike the microbe” collaboration of the iGEM Team US AFRL CarrollHS.
+
                      We participated in the “Mike the microbe” collaboration of the iGEM Team US AFRL CarrollHS.
−
                            They
+
                      They
−
                            sent us
+
                      sent us
−
                            their virtual mascots Mike the Microbe and Chia the Chitinase so we could take pictures with
+
                      their virtual mascots Mike the Microbe and Chia the Chitinase so we could take pictures with
−
                            them to
+
                      them to
−
                            connect
+
                      connect
−
                            all iGEM teams around the world via social media. It brought us great joy to join to this
+
                      all iGEM teams around the world via social media. It brought us great joy to join to this
−
                            collaboration and
+
                      collaboration and
−
                            let the two little buddies visit our daily life in our incubators for cyanobacteria.
+
                      let the two little buddies visit our daily life in our incubators for cyanobacteria.
−
                            <br>
+
                      <br>
−
                            <br>
+
                      <br>
−
                          </p>
+
                    </p>
−
                          <br>
+
                    <br>
−
                          <p>
+
                    <p>
−
                            <h4><b>Postcards Düsseldorf</b></h4> <!-- Postcard challenge-->
+
                      <h4><b>Postcards Düsseldorf</b></h4> <!-- Postcard challenge-->
−
                            iGEM Düsseldorf called for their traditional collaboration project, a long year postcard
+
                      iGEM Düsseldorf called for their traditional collaboration project, a long year postcard
−
                            campaign with the
+
                      campaign with the
−
                            purpose to educate and to show the public about the different topics and projects in
+
                      purpose to educate and to show the public about the different topics and projects in
−
                            Synthetic
+
                      Synthetic
−
                            Biology and
+
                      Biology and
−
                            especially regarding all the iGEM projects. With interesting designs and a short project
+
                      especially regarding all the iGEM projects. With interesting designs and a short project
−
                            abstract iGEM teams
+
                      abstract iGEM teams
−
                            could spread their ideas using the postcards to share their thinking to each other and the
+
                      could spread their ideas using the postcards to share their thinking to each other and the
−
                            society. Our
+
                      society. Our
−
                            title and postcard design was about our photosynthetic project „Syntex“. You can see
+
                      title and postcard design was about our photosynthetic project „Syntex“. You can see
−
                            Synechococcus elongatus
+
                      Synechococcus elongatus
−
                            UTEX 2973 direly needing a refreshing drink, a cute little draft from our team member Jonas.
+
                      UTEX 2973 direly needing a refreshing drink, a cute little draft from our team member Jonas.
−
                            <br>
+
                      <br>
−
                            <figure Style="text-align:center">
+
                      <figure Style="text-align:center">
−
                              <img src="    https://static.igem.org/mediawiki/2019/f/fe/T--marburg--Postcard.png"
+
                        <img src="    https://static.igem.org/mediawiki/2019/f/fe/T--marburg--Postcard.png"
−
                                alt="Postcard challenge">
+
                          alt="Postcard challenge">
−
                              <figcaption>
+
                        <figcaption>
−
                                Figure 2: Our Postcard for the Postcard collaboration from Düsseldorf.
+
                          Figure 2: Our Postcard for the Postcard collaboration from Düsseldorf.
−
                              </figcaption>
+
                        </figcaption>
−
                            </figure>
+
                      </figure>
−
                            <br>
+
                      <br>
−
                          </p>
+
                    </p>
−
                          <br>
+
                    <br>
−
                          <p>
+
                    <p>
−
                            <h5><b>iGEMxSDGs challenge</b></h5>
+
                      <h5><b>iGEMxSDGs challenge</b></h5>
−
                            IGEM Costa Rica, Tuebingen, and TAS Taipei created an iGEMxSDGs challenge as collaboration.
+
                      IGEM Costa Rica, Tuebingen, and TAS Taipei created an iGEMxSDGs challenge as collaboration.
−
                            They
+
                      They
−
                            asked us to
+
                      asked us to
−
                            choose four of the Sustainable Development Goals (SDGs) proposed by the United Nations (UN).
+
                      choose four of the Sustainable Development Goals (SDGs) proposed by the United Nations (UN).
−
                            We
+
                      We
−
                            joined this
+
                      joined this
−
                            collaboration challenge and spread it on our social media account. We decided on the
+
                      collaboration challenge and spread it on our social media account. We decided on the
−
                            following
+
                      following
−
                            goals:
+
                      goals:
−
                            <br>
+
                      <br>
−
                            <figure Style="text-align:center">
+
                      <figure Style="text-align:center">
−
                              <img src="  https://static.igem.org/mediawiki/2019/1/18/T--marburg--SDG_chalenge.png"
+
                        <img src="  https://static.igem.org/mediawiki/2019/1/18/T--marburg--SDG_chalenge.png"
−
                                alt="SGD Challenge">
+
                          alt="SGD Challenge">
−
                              <figcaption>
+
                        <figcaption>
−
                                Figure 3: SDG challenge.
+
                          Figure 3: SDG challenge.
−
                              </figcaption>
+
                        </figcaption>
−
                            </figure>
+
                      </figure>
−
                            <br>
+
                      <br>
−
                          </p>
+
                    </p>
−
                          <br>
+
                    <br>
  
−
                          <p>
+
                    <p>
−
                            <h5><b>Who wore it best? #labcoatchallenge</b></h5>
+
                      <h5><b>Who wore it best? #labcoatchallenge</b></h5>
−
                            The Stony Brook iGEM Team challenged us to design an outfit for a runway from a standard lab
+
                      The Stony Brook iGEM Team challenged us to design an outfit for a runway from a standard lab
−
                            coat.
+
                      coat.
−
                            The
+
                      The
−
                            outfit had to be created by tying, not using duct tape, cut or sew, etc. After designing our
+
                      outfit had to be created by tying, not using duct tape, cut or sew, etc. After designing our
−
                            outfits
+
                      outfits
−
                            four of
+
                      four of
−
                            our members presented their style on our ‘runway’ while paparazzi took their photos. As an
+
                      our members presented their style on our ‘runway’ while paparazzi took their photos. As an
−
                            outfit we
+
                      outfit we
−
                            chose a
+
                      chose a
−
                            dress highlighting the neckline, a shoulder free dress, a knotted dress and a skirt to bring
+
                      dress highlighting the neckline, a shoulder free dress, a knotted dress and a skirt to bring
−
                            glamour
+
                      glamour
−
                            to the
+
                      to the
−
                            daily routine in the labs. Especially our guys were rocking their outfits as you can see
+
                      daily routine in the labs. Especially our guys were rocking their outfits as you can see
−
                            here
+
                      here
−
                            x
+
                      x
−
                            <br>
+
                      <br>
−
                            <figure Style="text-align:center">
+
                      <figure Style="text-align:center">
−
                              <img style="height: 75ex; width: 60ex"
+
                        <img style="height: 75ex; width: 60ex"
−
                                src="https://static.igem.org/mediawiki/2019/7/74/T--marburg--Labcoat_Burgi.jpeg"
+
                          src="https://static.igem.org/mediawiki/2019/7/74/T--marburg--Labcoat_Burgi.jpeg" alt="labcoat">
−
                                alt="labcoat">
+
                        <figcaption>
−
                              <figcaption>
+
                          Figure 4: Our model Burgis for the labcoatchallenge.
−
                                Figure 4: Our model Burgis for the labcoatchallenge.
+
                        </figcaption>
−
                              </figcaption>
+
                      </figure>
−
                            </figure>
+
                      <br>
−
                            <br>
+
                    </p>
−
                          </p>
+
                  </div>
−
                        </div>
+
                  <br>
−
                        <br>
+
                  <br>
−
                        <br>
+
                </div>
−
                      </div>
+
              </div>
−
                    </div>
+
              <div class="sub" onclick="popup('rbn7')">
−
                    <div class="sub" onclick="popup('rbn7')">
+
                <div class="sub-header">
−
                      <div class="sub-header">
+
                  <h1>M E E T U P S</h1>
−
                        <h1>M E E T U P S</h1>
+
                  <hr>
−
                        <hr>
+
                </div>
−
                      </div>
+
                <div class="sub-content">
−
                      <div class="sub-content">
+
                  <p>
−
                        <p>
+
                  </p>
−
                        </p>
+
                </div>
−
                      </div>
+
              </div>
−
                    </div>
+
              <div id="rbn7" class="popup">
−
                    <div id="rbn7" class="popup">
+
                <div class="popup-container">
−
                      <div class="popup-container">
+
                  <div class="popup-header">
−
                        <div class="popup-header">
+
                    <h1 class="title">Meet ups</h1>
−
                          <h1 class="title">Meet ups</h1>
+
                    <button type="button" onclick="hide('rbn7')">X</button>
−
                          <button type="button" onclick="hide('rbn7')">X</button>
+
                  </div>
−
                        </div>
+
                  <div class="popup-content" style="text-align: justify;">
−
                        <div class="popup-content" style="text-align: justify;">
+
                    <div></div>
−
                          <div></div>
+
                    <p>
−
                          <p>
+
  
−
                            <h2><b>iGEM Spring Festival in Bonn</b></h2>
+
                      <h2><b>iGEM Spring Festival in Bonn</b></h2>
−
                            From 3rd – 5th Mai we met our iGEM colleagues for the Spring Festival in Bonn. Professors of
+
                      From 3rd – 5th Mai we met our iGEM colleagues for the Spring Festival in Bonn. Professors of
−
                            the
+
                      the
−
                            university of Bonn presented their research fields which gained huge interest. We heard
+
                      university of Bonn presented their research fields which gained huge interest. We heard
−
                            lectures
+
                      lectures
−
                            of immunology and neurosciences, as well as reviving mammoths. During the lectures, specific
+
                      of immunology and neurosciences, as well as reviving mammoths. During the lectures, specific
−
                            topics were deepened in small seminar groups, such as the ethical tenability of CRISPR
+
                      topics were deepened in small seminar groups, such as the ethical tenability of CRISPR
−
                            applied
+
                      applied
−
                            at humans and other animals. At the poster sessions we got the first time in contact with
+
                      at humans and other animals. At the poster sessions we got the first time in contact with
−
                            the
+
                      the
−
                            other teams’ projects. Afterwards we had the opportunity to vote for the best project, which
+
                      other teams’ projects. Afterwards we had the opportunity to vote for the best project, which
−
                            iGEM Düsseldorf won. Besides that, the meetup was filled with a lot of awarding categories.
+
                      iGEM Düsseldorf won. Besides that, the meetup was filled with a lot of awarding categories.
−
                            We
+
                      We
−
                            competed in the categories for “Best Video”, “Best Poster”, “Best Project” and a single
+
                      competed in the categories for “Best Video”, “Best Poster”, “Best Project” and a single
−
                            person
+
                      person
−
                            had the chance to win the award for “Best Meme”. The iGEM Team Bonn offered spontaneously a
+
                      had the chance to win the award for “Best Meme”. The iGEM Team Bonn offered spontaneously a
−
                            little football table tournament, where two of our team members won. In the evening we
+
                      little football table tournament, where two of our team members won. In the evening we
−
                            learnt a
+
                      learnt a
−
                            lot about Bonn as a city from a guide living there for ages. Afterwards, the iGEM Teams met
+
                      lot about Bonn as a city from a guide living there for ages. Afterwards, the iGEM Teams met
−
                            in
+
                      in
−
                            several groups in restaurants and bars. We merged with iGEM Kaiserslautern and exchanged our
+
                      several groups in restaurants and bars. We merged with iGEM Kaiserslautern and exchanged our
−
                            projects and ideas for the rest of the evening.
+
                      projects and ideas for the rest of the evening.
−
                            <br>
+
                      <br>
−
                            On the second day, the iGEM Team Bonn offered a competition out of three rounds, where we
+
                      On the second day, the iGEM Team Bonn offered a competition out of three rounds, where we
−
                            competed against other teams. We were asked iGEM specific questions, had to solve riddles
+
                      competed against other teams. We were asked iGEM specific questions, had to solve riddles
−
                            for an
+
                      for an
−
                            escape room and finished a lab challenge afterwards. Our team could compete until the end
+
                      escape room and finished a lab challenge afterwards. Our team could compete until the end
−
                            and
+
                      and
−
                            won the prize! Thanks again to Doulix for this awesome prize!
+
                      won the prize! Thanks again to Doulix for this awesome prize!
−
                            <br>
+
                      <br>
−
                            We particularly thank the iGEM Team Bonn for the huge effort and the great meetup!
+
                      We particularly thank the iGEM Team Bonn for the huge effort and the great meetup!
−
                            <br>
+
                      <br>
−
                            <figure Style="text-align:center">
+
                      <figure Style="text-align:center">
−
                              <img style="height: 50ex; width: 65ex"
+
                        <img style="height: 50ex; width: 65ex"
−
                                src="https://static.igem.org/mediawiki/2019/a/a9/T--marburg--meet_u_bonn.png"
+
                          src="https://static.igem.org/mediawiki/2019/a/a9/T--marburg--meet_u_bonn.png"
−
                                alt="Erlangen meetup">
+
                          alt="Erlangen meetup">
−
                              <figcaption>
+
                        <figcaption>
−
                                Fig. 1: Two representatives of Doulix celebrating with us.
+
                          Fig. 1: Two representatives of Doulix celebrating with us.
−
                              </figcaption>
+
                        </figcaption>
−
                            </figure>
+
                      </figure>
  
−
                            <br>
+
                      <br>
  
−
                            <h3><b>Erlangen collaboration meetup</b></h3>
+
                      <h3><b>Erlangen collaboration meetup</b></h3>
−
                            On the 17th – 19th of May we attended the collaboration meetup hosted by the iGEM Team
+
                      On the 17th – 19th of May we attended the collaboration meetup hosted by the iGEM Team
−
                            Erlangen
+
                      Erlangen
−
                            2019. There, we met the iGEM teams of Straßbourg, Tübingen, Vienna and of course Erlangen.
+
                      2019. There, we met the iGEM teams of Straßbourg, Tübingen, Vienna and of course Erlangen.
−
                            Apart
+
                      Apart
−
                            from listening to the interesting talks from the various professors there, we made a lot of
+
                      from listening to the interesting talks from the various professors there, we made a lot of
−
                            new
+
                      new
−
                            friends and took the chance to talk about possible collaborations with the other teams and
+
                      friends and took the chance to talk about possible collaborations with the other teams and
−
                            also
+
                      also
−
                            promote our collaborations, such as the colony picking project and the Golden Gate webinar
+
                      promote our collaborations, such as the colony picking project and the Golden Gate webinar
−
                            (evtl. verlinken). We would like to thank iGEM Team Erlangen very much for organizing this
+
                      (evtl. verlinken). We would like to thank iGEM Team Erlangen very much for organizing this
−
                            wonderful meetup and are happy to see them soon in Boston.
+
                      wonderful meetup and are happy to see them soon in Boston.
−
                            <br>
+
                      <br>
  
−
                            <figure Style="text-align:center">
+
                      <figure Style="text-align:center">
−
                              <img style="height: 40ex; width: 65ex"
+
                        <img style="height: 40ex; width: 65ex"
−
                                src="https://static.igem.org/mediawiki/2019/0/01/T--marburg--meet_up_erlangen.jpeg"
+
                          src="https://static.igem.org/mediawiki/2019/0/01/T--marburg--meet_up_erlangen.jpeg"
−
                                alt="Erlangen meetup">
+
                          alt="Erlangen meetup">
−
                              <figcaption>
+
                        <figcaption>
−
                                Fig. 2: Group photo from the Erlangen mett up (Michael Burgis from Team Marburg in the
+
                          Fig. 2: Group photo from the Erlangen mett up (Michael Burgis from Team Marburg in the
−
                                left
+
                          left
−
                                side).
+
                          side).
−
                              </figcaption>
+
                        </figcaption>
−
                            </figure>
+
                      </figure>
  
−
                            <br>
+
                      <br>
  
−
                            <h><b>Düsseldorf meetup</b></h>
+
                      <h><b>Düsseldorf meetup</b></h>
−
                            <br>From 5th – 7th July we went to the HHU for the German meetup. The calendar offered lots
+
                      <br>From 5th – 7th July we went to the HHU for the German meetup. The calendar offered lots
−
                            of
+
                      of
−
                            lectures with interesting topics such as the Excellent Cluster of Prof. Andreas Weber
+
                      lectures with interesting topics such as the Excellent Cluster of Prof. Andreas Weber
−
                            “CEPLAS”
+
                      “CEPLAS”
−
                            but also non-scientific aspects like how to do presentations. We tried to visit all of them
+
                      but also non-scientific aspects like how to do presentations. We tried to visit all of them
−
                            while presenting our poster in the hall. There we got in touch with other iGEM Teams also
+
                      while presenting our poster in the hall. There we got in touch with other iGEM Teams also
−
                            learnt
+
                      learnt
−
                            about their projects. We promoted our Webinar project and laid the foundation stone for
+
                      about their projects. We promoted our Webinar project and laid the foundation stone for
−
                            future
+
                      future
−
                            collaborations. On the second day, iGEM Düsseldorf organized a panel discussion about the
+
                      collaborations. On the second day, iGEM Düsseldorf organized a panel discussion about the
−
                            issues
+
                      issues
−
                            concerning green genetic engineering. Thereby, we got great input on how to structure our
+
                      concerning green genetic engineering. Thereby, we got great input on how to structure our
−
                            panel
+
                      panel
−
                            discussion and which subjects should be mentioned. Beside the regular program organized by
+
                      discussion and which subjects should be mentioned. Beside the regular program organized by
−
                            iGEM
+
                      iGEM
−
                            Düsseldorf we used a lot of chances to get in contact with other teams. After the
+
                      Düsseldorf we used a lot of chances to get in contact with other teams. After the
−
                            presentations
+
                      presentations
−
                            a couple of tourist guides showed us the beautiful old town of Düsseldorf and explained some
+
                      a couple of tourist guides showed us the beautiful old town of Düsseldorf and explained some
−
                            interesting historical facts. Afterwards we had again the possibility to meet with other
+
                      interesting historical facts. Afterwards we had again the possibility to meet with other
−
                            iGEMers
+
                      iGEMers
−
                            in a pub and talk about the projects, especially to find weaknesses or opportunities for
+
                      in a pub and talk about the projects, especially to find weaknesses or opportunities for
−
                            collaborations. This really went successfully due to the time we spend with iGEM Team Aachen
+
                      collaborations. This really went successfully due to the time we spend with iGEM Team Aachen
−
                            and
+
                      and
−
                            Kaiserslautern. The iGEM Team Düsseldorf also arranged a game contest between the teams,
+
                      Kaiserslautern. The iGEM Team Düsseldorf also arranged a game contest between the teams,
−
                            where
+
                      where
−
                            we lost in the final to our good friends from Potsdam. Congrats! <br>
+
                      we lost in the final to our good friends from Potsdam. Congrats! <br>
  
−
                            <figure Style="text-align:center">
+
                      <figure Style="text-align:center">
−
                              <img style="height: 70ex; width: 50ex"
+
                        <img style="height: 70ex; width: 50ex"
−
                                src="https://static.igem.org/mediawiki/2019/e/e4/T--marburg--meet_up_Duesseldorf.jpeg"
+
                          src="https://static.igem.org/mediawiki/2019/e/e4/T--marburg--meet_up_Duesseldorf.jpeg"
−
                                alt="Du meetup">
+
                          alt="Du meetup">
−
                              <figcaption>
+
                        <figcaption>
−
                                Fig. 3: Team photo from the Düsseldorf meetup.
+
                          Fig. 3: Team photo from the Düsseldorf meetup.
−
                              </figcaption>
+
                        </figcaption>
−
                            </figure>
+
                      </figure>
−
                            <br>Thanks again to iGEM Düsseldorf for the huge effort and the great meetup! <br>
+
                      <br>Thanks again to iGEM Düsseldorf for the huge effort and the great meetup! <br>
−
                          </p>
+
                    </p>
−
                          <br>
+
                    <br>
  
−
                          <h><b> Vilnius, Lithuania – Biohackathon Lab App 2019</b></h>
+
                    <h><b> Vilnius, Lithuania – Biohackathon Lab App 2019</b></h>
−
                          <br>In August 2019 our Team participated in the Biohackathon Lab App, organized by the
+
                    <br>In August 2019 our Team participated in the Biohackathon Lab App, organized by the
−
                          Vilnius-Lithuania Team 2019. Together with the iGEM Teams Stockholm and UCopenhagen, as well
+
                    Vilnius-Lithuania Team 2019. Together with the iGEM Teams Stockholm and UCopenhagen, as well
−
                          as
+
                    as
−
                          several non-iGEM Teams we sat together for a weekend packed with problems, ideas and neat
+
                    several non-iGEM Teams we sat together for a weekend packed with problems, ideas and neat
−
                          solutions.
+
                    solutions.
−
                          With the task of creating an application that tackles a self-found problem, we sat together
+
                    With the task of creating an application that tackles a self-found problem, we sat together
−
                          and
+
                    and
−
                          brainstormed about what could be done.
+
                    brainstormed about what could be done.
−
                          <br>
+
                    <br>
−
                          During this time we were starting to work more with the Opentrons OT-2 and soon realized that
+
                    During this time we were starting to work more with the Opentrons OT-2 and soon realized that
−
                          some
+
                    some
−
                          of our biologists had trouble integrating protocols in the robot themselves, as this is
+
                    of our biologists had trouble integrating protocols in the robot themselves, as this is
−
                          primarily
+
                    primarily
−
                          done by writing the protocol in Python and pushing it directly to the robot – no convenient
+
                    done by writing the protocol in Python and pushing it directly to the robot – no convenient
−
                          application to change parameters, such as the amount of reactions,was included in the
+
                    application to change parameters, such as the amount of reactions,was included in the
−
                          protocols.
+
                    protocols.
−
                          In our heads the idea to have a user-friendly GUI grew more and more and soon we found
+
                    In our heads the idea to have a user-friendly GUI grew more and more and soon we found
−
                          ourselves
+
                    ourselves
−
                          standing in the Vilnius University Life Sciences Center.
+
                    standing in the Vilnius University Life Sciences Center.
−
                          <br>“We” were two biologists and one mathematician, so we talked to some of the other
+
                    <br>“We” were two biologists and one mathematician, so we talked to some of the other
−
                          participants
+
                    participants
−
                          that did not have a team yet and found the perfect fit to complement our small team: Nour
+
                    that did not have a team yet and found the perfect fit to complement our small team: Nour
−
                          Alsamsam
+
                    Alsamsam
−
                          was happy to join us for this weekend and provided us with his coding skills – huge thank you
+
                    was happy to join us for this weekend and provided us with his coding skills – huge thank you
−
                          to
+
                    to
−
                          you, Nour!
+
                    you, Nour!
−
                          Together we tried our best to come up with a suitable app and presented it in the end, having
+
                    Together we tried our best to come up with a suitable app and presented it in the end, having
−
                          more
+
                    more
−
                          ideas in our heads than we could implement in such a short time – it was clear for us, that we
+
                    ideas in our heads than we could implement in such a short time – it was clear for us, that we
−
                          would continue with this project later on!
+
                    would continue with this project later on!
−
                          <br>In conclusion, the Biohackathon was an awesome and productive meeting of the iGEM Teams
+
                    <br>In conclusion, the Biohackathon was an awesome and productive meeting of the iGEM Teams
−
                          Stockholm, Copenhagen, Vilnius and Marburg. We talked about our projects, offered each other
+
                    Stockholm, Copenhagen, Vilnius and Marburg. We talked about our projects, offered each other
−
                          feedback on current issues in the lab and shared many ideas.
+
                    feedback on current issues in the lab and shared many ideas.
−
                          We are very grateful that we got the opportunity to meet so many experts from different
+
                    We are very grateful that we got the opportunity to meet so many experts from different
−
                          fields,
+
                    fields,
−
                          which really helped getting a fresh perspective on our project and are now looking forward to
+
                    which really helped getting a fresh perspective on our project and are now looking forward to
−
                          meet
+
                    meet
−
                          the other teams again at the Giant Jamboree!
+
                    the other teams again at the Giant Jamboree!
−
                          <br>
+
                    <br>
−
                          <figure Style="text-align:center">
+
                    <figure Style="text-align:center">
−
                            <img src="Pic" alt="Vilnius hacker">
+
                      <img src="Pic" alt="Vilnius hacker">
−
                            <figcaption>
+
                      <figcaption>
−
                              Fig. 4: Meet up Vilnius, Lithuania .
+
                        Fig. 4: Meet up Vilnius, Lithuania .
−
                            </figcaption>
+
                      </figcaption>
−
                          </figure>
+
                    </figure>
  
−
                          </p>
+
                    </p>
−
                        </div>
+
−
                        <br>
+
−
                        <br>
+
−
                      </div>
+
−
                    </div>
+
 
                   </div>
 
                   </div>
−
               </section>
+
                  <br>
 +
                  <br>
 +
                </div>
 +
               </div>
 
             </div>
 
             </div>
−
          </div>
+
      </section>
−
          </body>
+
    </div>
 +
  </div>
 +
  </body>
  
 
</html>
 
</html>
 
{{Marburg/footer}}
 
{{Marburg/footer}}

Revision as of 20:32, 21 October 2019

C O L L A B O R A T I O N S

Through this iGEM year we interacted with so many iGEM Teams all around the world. It was a pleasure working with all the different teams and learn from each other. We got so many impressions about other projects and hope we could help with our collaborations. A huge Thank you for every team collaboration!

C O L O N Y
P I C T U R E S

In our colony picking collaboration we asked other iGEM teams to supply us with data for our picking algorithm. Thanks to this group effort we could build our colony picking unit.

T H EAG O L D E NAG A T E

C O L L A B O R A T I O N