Team:TU Dresden/Experiments
Beaming BioBricks from Space...
Protocols
Use a BioBrick from the Disctribution kit
The fastest gDNA extraction protocol for PCR with cellulose
Make a stock of electrocompetent cells from E. coli GB05 with flash freezing
Electroporate E. coli GB05
Heat shock transformation with NEB E.coli 5α
Optimized miniprep protocol for ZymoPURE Plasmid Miniprep Kit
No-Kit miniprep protocol
Enzyme Saver Mastermix for gel electrophoresis analysis
Agarose gel preparation and run
BioBrick expression protocol in E. coli pRARE T7
Cell lysis and Purification of HRP-Strep
Expression and purification of dCas9 fusion proteins via Amylose Resin and Strep-tag
Activity assay for HRP with TMB
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