Team:Strasbourg/Protocols

iGEM

In-vitro transcription protocol

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Protocols BH3 Project

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Summary

  1. Bacterial transformation: p.1-3
  2. Bacterial Glycerol Stocks for Long-term Storage: p.4
  3. Inoculating a Liquid Bacterial Culture: p.5
  4. PCR: p.6
  5. Gibson assembly: p.7-8
  6. Triple-Bacterial transformation: p.9
  7. Competent cells (XL1 Blue): p10-11
  8. Growth test: p.12-13
  9. LB Medium: p.14
  10. Petry dishes (LB + agar): p.15
  11. Double digestion: p.16
  12. Beta galactosidase assay (Using cuvettes): p.17-20
  13. Beta galactosidase assay (Using 96 plate reader): p.21-23

    14. Protocols LexA Project

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    Summary

    1. Site-directed mutagenesis Taq'Ozyme: p.1-2
    2. Site-directed mutagenesis Pfu pol: p.3-4
    3. Agarose gel electrophoresis: p.5
    4. CaCl2 competent cells: p.6
    5. Transformation of competent cells: p.7
    6. Monarch Plasmid DNA Miniprep Kit Protocol (NEB#T1010): p.8
    7. Gibson Assembly (E5510): p9