Team:Strasbourg/Parts

iGEM

Parts Overview

iGEM Strasbourg project was concentrated on establishing two aptazyme based three-hybrid systems. For this, we linked the aptazyme to MS2 and PP7 stem-loops which represent a big portion of our basic parts. Next to this construct, we tried to produce fusion MS2-LexAmut and PP7-LexA proteins as the components of one of the proposed bacterial three hybrid systems.
Our team added to registry two different types of aptazymes: anti-theophylline switch-off (cleaves itself in absence of ligand) and anti-guanine switch-on (cleaves itself in presence of ligand). Compared to previously described anti-theophylline structures our aptazymes have 100% cleavage activity in vitro. Moreover, guanine aptazyme is the first switch-on aptazyme in the history of the iGEM registry. Our team also added the sequences of inactive aptazymes which are the perfect control for investigating aptazymes activity.

Table Parts

Name	Type	Description	Designer	Length
BBa_K2923000	RNA	MS2_MS2-PP7_PP7 RNA	Kateryna LEN	130
BBa_K2923001	RNA	Theophylline actif aptazyme	claire husser	94
BBa_K2923002	RNA	Theophylline inactif aptazyme	claire husser	94
BBa_K2923003	RNA	Guanine actif aptazyme	claire husser	138
BBa_K2923008	RNA	Guanine inactif aptazyme	claire husser	138
BBa_K2923009	Coding	LexA WT repressor	Kateryna LEN	255
BBa_K2923010	Coding	LexA mutated repressor	Kateryna LEN	255
BBa_K2923011	Coding	MS2 coat protein	Kateryna LEN	381
BBa_K2923012	Coding	PP7 coat protein	Kateryna LEN	387
BBa_K2923013	Composite	MS2cp-LexaAmut	Kateryna LEN	680
BBa_K2923014	Scar	Linker	Kateryna LEN	30
BBa_K2923015	Composite	PP7cp-LexA WT	Kateryna LEN	686
BBa_K2923016	RNA	Two PP7 stem-loops	Kateryna LEN	60
BBa_K2923017	RNA	Two MS2 stem-loops	Kateryna LEN	70
BBa_K2923018	Composite	MS2-MS2-Theophylline active aptazyme-PP7-PP7	Kateryna LEN	240
BBa_K2923019	Composite	MS2-MS2-Theophylline inactive aptazyme-PP7-PP7	Kateryna LEN	240
BBa_K2923020	Composite	MS2-MS2-Guanine actif aptazyme-PP7-PP7	Kateryna LEN	284
BBa_K2923021	Composite	MS2-MS2-Guanine inactif aptazyme-PP7-PP7	Kateryna LEN	284