Team:SDU CHINA/Improve
Our team has optimized the BBa_S04615 part. This part consists of the promoter B0034 and the BBa_K592001.
In 2018, the SDU-CHINA team optimized the K592001 part to reduce its leakage under the red light. We have improved its promoters this year, which has further bettered the performance of this component.
The component BBa_K2627000 proved the progress of the optimized CcaS by sufficient characterization, so we directly replaced K592001 in BBa_S04615 with the optimized K2627000 when characterizing this component. In 2014, Schmidl S R , Sheth R U, Wu A, et al. optimized CcaS by constructing an RBS library. This document has fully proven that the performance of components with optimized RBS is better than S04615. In 2019, our team SDU-CHINA has further improved the components based on them. We have introduced site-directed mutagenesis to the improved promoter, further bettering the performance based on all previous studies.
The plasmid carrying our RBS is called 5-10#10. There is less fluorescence intensity than #10 under light of 640-650 nm wavelength.
Reference
[1]Schmidl, S. R. , Sheth, R. U. , Wu, A. , & Tabor, J. J. . (2014). Refactoring and optimization of light-switchable\r, escherichia coli\r, two-component systems. ACS Synthetic Biology, 3(11), 820-831.