Team:Oxford/Protocols

Protocols

Benchling provided a convenient repository for us to ensure consistency in our wet lab protocols. Below are some of the most commonly used protocols over this project.

Affinity Chromatography - Ni-NTA

Affinity Chromatography - Spy&Go

Cell Harvesting and Lysis

Chemically Competent E. coli Cells

Colony PCR - L. reuteri

Dialysis

Electroporation - L. reuteri - Quadram Institute

Electroporation - L. reuteri - Aukrust et al.

Fluorescence Measurement

Gel Extraction

Gel Preparation

Gibson Assembly

Heat Shock Transformation - E. coli

In-Gel SpyCatcher Blot

Mass Spectrometry

OD600 Kill Assay

PCR with Q5 Polymerase

SDS-PAGE

Western Blot