| BBa_K3183000 |
Regulatory |
erm |
Erythromycin Constitutive Promoter for Lactobacillus sp. |
Promoter |
| BBa_K3183001 |
Regulatory |
slp |
Surface (S)-Layer Protein Constitutive Promoter for Lactobacillus sp. |
Promoter |
| BBa_K3183002 |
Regulatory |
ldh |
Lactate Dehydrogenase Constitutive Promoter for Lactobacillus sp. |
Promoter |
| BBa_K3183003 |
Regulatory |
AgrProm |
Putative C. difficile AgrA2 Promoter |
Promoter |
| BBa_K3183004 |
Coding |
AgrA |
AgrA2 Quorum Sensing Transcription Factor; C. difficile |
2 component signalling system cytoplasmic component |
| BBa_K3183005 |
Coding |
AgrC |
AgrC2 Quorum Sensing Histidine Kinase; C. difficile |
2 component signalling system cytoplasmic component |
| BBa_K3183006 |
Coding |
AgrB |
AgrB Quorum Sensing Processing Protein; C. difficile |
AIP production |
| BBa_K3183007 |
Coding |
AgrD |
AgrD Quorum Sensing ; C. difficile |
AIP production and processing |
| BBa_K3183008 |
Secretion tag |
slpMOD |
slpMOD Lactobacillus Secretion Tag |
Secretion |
| BBa_K3183010 |
Coding |
mClover3 |
mClover3 Fluorescent Protein, Codon Optimized for L. reuteri |
Reporter gene |
| BBa_K3183011 |
Coding |
mGFP5 |
Modified Green Fluorescent Protein, Codon Optimized for L. reuteri |
Reporter gene |
| BBa_K3183009 |
Coding |
CD27L |
CD27L Endolysin Targetting C. difficile for E. coli |
Killing C. difficile |
| BBa_K3183012 |
Coding |
CD27L1-179 |
CD27L Endolysin Residues 1-179 Targetting C. difficile for E. coli |
Killing C. difficile |
| BBa_K1159201 |
Purification tag |
SpyTag |
N/A |
Purification |
| BBa_K3183013 |
Purification tag |
SpyTag L reuteri |
N/A |
Purification |
|
| BBa_K895005 |
Coding |
Dundee CD27L |
N/A |
Killing C. difficile |
| BBa_K3183014 |
Linker |
GSG linker |
Gly-Ser-Gly Protein Domain Linker for E. coli |
Linker |
| BBa_K3183015 |
Linker |
GSG linker |
Gly-Ser-Gly Protein Domain Linker for L. reuteri |
Linker |
| BBa_M0021 |
Purification tag |
6His |
6-Histidine Tag Codon-Optimised for E. coli |
Purification |
| BBa_K3183016 |
Purification tag |
6His |
6-Histidine Tag Codon-Optimised for L. reuteri |
Purification |
| BBa_K3183017 |
Coding |
CD27L L. reuteri |
CD27L Endolysin Targetting C. difficile Optimized for L. reuteri |
Killing C. difficile |
| BBa_K3183018 |
Coding |
CD27L1-179 L. reuteri |
CD27L Endolysin Targetting C. difficile Optimized for L. reuteri |
Killing C. difficile |
| BBa_K3183019 |
DNA |
L. reuteri spacer |
Includes SD box |
Spacer |
| BBa_K3183020 |
DNA |
E. coli spacer |
includes high-efficiency ribosome binding site |
Spacer |
| BBa_K3183021 |
Terminator |
T7 Terminator (Double) |
Phage Terminators T7 and T1rrb Terminator fused for use in L. reuteri |
Terminator |
BBa_K3183022 |
Terminator |
T7 Terminator |
Phage Terminator T7 for use in L. reuteri in conjunction with T1rrb Terminator |
Terminator |
BBa_K3183023 |
Terminator |
T1 Terminator rrnB |
Phage Terminator T1rrb for use in L. reuteri in conjunction with T7 Terminator |
Terminator |
BBa_K3183024 |
Tag |
HA Tag (L. reuteri) |
Standard Hemagglutinin Tag optimised for L. reuteri |
Tag |
BBa_K3183025 |
Protein_DomainMSG |
Start MSG E. coli |
Start Methionine-Glycine-Serine Linker |
Enhances protein lifetime |
BBa_K3183026 |
DNA |
AgrAC Spacer |
Separates two proteins of Agr two component signalling system |
Spacer+RBS |
BBa_K3183027 |
Composite |
AgrB/D Spacer |
Spacer between AIP producing genes in C. diff |
Spacer |
BBa_K3183028 |
Reporter |
erm-mClover3 |
reporter gene compromised of P-erm and mClover3 fluorescent protein. |
Promoter characterisation and quantification |
BBa_K2141000 |
Plasmid |
pET28A |
pET28a(+) expression plasmid kanamycin resistance |
Protein expression induction |
BBa_K3183050 |
Plasmid |
pTRKH3 |
shuttle vector for L. reuteri |
Backbone with erythromycin resistance |
BBa_K3183100 |
Reporter |
erm-GFP |
Erythromycin Promoter Reporter Gene + GFP L. reuteri |
Expression + Fluorescence |
BBa_K3183101 |
Reporter |
ldh-mClover3 |
Lactate Dehydrogenase Promoter Reporter Gene |
Promoter Characterisation |
BBa_K3183102 |
Generator |
slp-AgrC-AgrA |
AgrAC Translational Unit for L. reuteri |
Expression |
BBa_K3183103 |
Generator |
erm-CD27L (L. reuteri) |
CD27L and mClover3 Under Erythromycin Promoter Control |
Expression + secretion |
BBa_K3183104 |
Regulatory |
Agr-Prom-mClover3 |
Putative Agr Promoter for C. difficile Fused with mClover3 |
Promoter activated by AgrAC signalling system |
BBa_K3183200 |
DNA |
CD27L plus Tags |
CD27L with N-Terminal 6-His Tag and SpyTag |
Killing of C. difficile |
BBa_K3183201 |
DNA |
CD27L plus Tags |
Truncated CD27L 1-179 for E. coli with 6-His and SpyTag |
Killing of C. difficile |
BBa_K3183202 |
DNA |
AgrBD E. coli |
pET28A-AgrBD |
Purification |
BBa_K3183203 |
DNA |
SpyTag-mClover 6His |
mClover3 with SpyTag and 6-His E. coli |
Reporter gene + purification quantification |
BBa_K3183300 |
Composite |
slp-AgrC-AgrA Agr Prom - CD27L (L. reuteri) Tag |
includes SpyTag and His tag |
C. difficile Detection and CD27L Secretion |
