Team:Oxford/Parts

Registry # Type Common Name Description Function
BBa_K3183000 Regulatory erm Erythromycin Constitutive Promoter for Lactobacillus sp. Promoter
BBa_K3183001 Regulatory slp Surface (S)-Layer Protein Constitutive Promoter for Lactobacillus sp. Promoter
BBa_K3183002 Regulatory ldh Lactate Dehydrogenase Constitutive Promoter for Lactobacillus sp. Promoter
BBa_K3183003 Regulatory AgrProm Putative C. difficile AgrA2 Promoter Promoter
BBa_K3183004 Coding AgrA AgrA2 Quorum Sensing Transcription Factor; C. difficile 2 component signalling system cytoplasmic component
BBa_K3183005 Coding AgrC AgrC2 Quorum Sensing Histidine Kinase; C. difficile 2 component signalling system cytoplasmic component
BBa_K3183006 Coding AgrB AgrB Quorum Sensing Processing Protein; C. difficile AIP production
BBa_K3183007 Coding AgrD AgrD Quorum Sensing ; C. difficile AIP production and processing
BBa_K3183008 Secretion tag slpMOD slpMOD Lactobacillus Secretion Tag Secretion
BBa_K3183010 Coding mClover3 mClover3 Fluorescent Protein, Codon Optimized for L. reuteri Reporter gene
BBa_K3183011 Coding mGFP5 Modified Green Fluorescent Protein, Codon Optimized for L. reuteri Reporter gene
BBa_K3183009 Coding CD27L CD27L Endolysin Targetting C. difficile for E. coli Killing C. difficile
BBa_K3183012 Coding CD27L1-179 CD27L Endolysin Residues 1-179 Targetting C. difficile for E. coli Killing C. difficile
BBa_K1159201 Purification tag SpyTag N/A Purification
BBa_K3183013 Purification tag SpyTag L reuteri N/A Purification
BBa_K895005 Coding Dundee CD27L N/A Killing C. difficile
BBa_K3183014 Linker GSG linker Gly-Ser-Gly Protein Domain Linker for E. coli Linker
BBa_K3183015 Linker GSG linker Gly-Ser-Gly Protein Domain Linker for L. reuteri Linker
BBa_M0021 Purification tag 6His 6-Histidine Tag Codon-Optimised for E. coli Purification
BBa_K3183016 Purification tag 6His 6-Histidine Tag Codon-Optimised for L. reuteri Purification
BBa_K3183017 Coding CD27L L. reuteri CD27L Endolysin Targetting C. difficile Optimized for L. reuteri Killing C. difficile
BBa_K3183018 Coding CD27L1-179 L. reuteri CD27L Endolysin Targetting C. difficile Optimized for L. reuteri Killing C. difficile
BBa_K3183019 DNA L. reuteri spacer Includes SD box Spacer
BBa_K3183020 DNA E. coli spacer includes high-efficiency ribosome binding site Spacer
BBa_K3183021 Terminator T7 Terminator (Double) Phage Terminators T7 and T1rrb Terminator fused for use in L. reuteri Terminator
BBa_K3183022 Terminator T7 Terminator Phage Terminator T7 for use in L. reuteri in conjunction with T1rrb Terminator Terminator
BBa_K3183023 Terminator T1 Terminator rrnB Phage Terminator T1rrb for use in L. reuteri in conjunction with T7 Terminator Terminator
BBa_K3183024 Tag HA Tag (L. reuteri) Standard Hemagglutinin Tag optimised for L. reuteri Tag
BBa_K3183025 Protein_DomainMSG Start MSG E. coli Start Methionine-Glycine-Serine Linker Enhances protein lifetime
BBa_K3183026 DNA AgrAC Spacer Separates two proteins of Agr two component signalling system Spacer+RBS
BBa_K3183027 Composite AgrB/D Spacer Spacer between AIP producing genes in C. diff Spacer
BBa_K3183028 Reporter erm-mClover3 reporter gene compromised of P-erm and mClover3 fluorescent protein. Promoter characterisation and quantification
BBa_K2141000 Plasmid pET28A pET28a(+) expression plasmid kanamycin resistance Protein expression induction
BBa_K3183050 Plasmid pTRKH3 shuttle vector for L. reuteri Backbone with erythromycin resistance
BBa_K3183100 Reporter erm-GFP Erythromycin Promoter Reporter Gene + GFP L. reuteri Expression + Fluorescence
BBa_K3183101 Reporter ldh-mClover3 Lactate Dehydrogenase Promoter Reporter Gene Promoter Characterisation
BBa_K3183102 Generator slp-AgrC-AgrA AgrAC Translational Unit for L. reuteri Expression
BBa_K3183103 Generator erm-CD27L (L. reuteri) CD27L and mClover3 Under Erythromycin Promoter Control Expression + secretion
BBa_K3183104 Regulatory Agr-Prom-mClover3 Putative Agr Promoter for C. difficile Fused with mClover3 Promoter activated by AgrAC signalling system
BBa_K3183200 DNA CD27L plus Tags CD27L with N-Terminal 6-His Tag and SpyTag Killing of C. difficile
BBa_K3183201 DNA CD27L plus Tags Truncated CD27L 1-179 for E. coli with 6-His and SpyTag Killing of C. difficile
BBa_K3183202 DNA AgrBD E. coli pET28A-AgrBD Purification
BBa_K3183203 DNA SpyTag-mClover 6His mClover3 with SpyTag and 6-His E. coli Reporter gene + purification quantification
BBa_K3183300 Composite slp-AgrC-AgrA Agr Prom - CD27L (L. reuteri) Tag includes SpyTag and His tag C. difficile Detection and CD27L Secretion