Team:NWU-China/Improve
Improved Parts
We fused amilCP ( BBa_K592009) to the signal peptide Kp-Sp (new part is BBa_K2981007) from actinomycetes (Yanbing Cui et al., 2017) and transferred the plasmid into E. coli BL-21. Incubate overnight in LB medium at 37℃ , 200 rpm. The control group was an amilCP without the fusion signal peptide Kp-Sp. 15 ml of the bacterial solution was taken from each group and centrifuged at 8000 rpm. It was observed that the supernatant of the experimental group showed a clear blue color, the supernatant of the control group had no color, and the value of OD588 was measured. After that, we collected the bacteria of the experimental group and used ultrasonic lysis to measure OD588 using a microplate reader. We found that about 30% of amilCP is secreted outside the cell.
result comparison chart