Team:NWU-China/Composite Part

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Composite Parts

 

Number	Part Number	Part Name	Long Description
[1]	BBa_K2981007	Kp-Sp-amilCP	The secreting peptide Kp-SP was added to the N-terminus of the pigment protein - amilCP, and realized the transition of the amilCP from intracellular to extracellular.
[2]	BBa_K2981009	Plac-RBS-Kp-Sp-amilCP-Terminator	we connect the Kp-SP secreting peptide at the N-terminus of the pigment protein, and realize the transition of the pigment protein from intracellular to extracellular..This section is used for the measurement of data in the experiment.
[3]	BBa_K2981010	ParoF-RBS -RFP-terminator	This part uses RFP as a reporter gene and is regulated by ParoF. We aimed to measure the intensity of RFP expression by adding phenylacetic acid to treat bacteria, and to eliminate the influence of catabolic by-products of Phe metabolism in urine on experimental results.
[4]	BBa_K2981011	PtyrP-RBS -GFP-terminator	This part expresses the reporter gene GFP by promoter for PtyrP. Our aim was to eliminate the effects of catabolic by-products of Tyr metabolism in urine on the experimental results by adding PPA to treat bacteria and measuring the intensity of GFP expression. In addition, the background expression of PtyrP was determined by the fluorescence intensity of GFP normally expressed by this construct.
[5]	BBa_K2981013	PtyrP-RBS -GFP-terminator - Plac- RBS -TyrR-terminator	In the presence of Phe and ATP, TyrR strongly induced the expression of tyrP. This plasmid incorporates PtyrP and TyrR, and a standard curve was constructed by measuring the fluorescence expression intensity of RFP by gradient addition of Tyr in M9 medium.
[6]	BBa_K2981014	ParoF-RBS -RFP-terminator–Plac- RBS -TyrR-terminator	In the presence of Tyr and ATP, the TyrR dimer self-associates to form a hexamer, thereby inhibiting aroF. This plasmid was constructed to obtain a standard curve by measuring the fluorescence expression intensity of RFP by gradient addition of Tyr in M9 medium.
[7]	BBa_K2981015	ParoF-RBS -RFP-terminator– - PtyrP-RBS -GFP-terminator - Plac- RBS -TyrR-terminator	The biosynthesis of Phe and Tyr in E. coli is controlled by the DNA binding protein TyrR. In the presence of Tyr and ATP, the TyrR dimer self-associates to form a hexamer, thereby inhibiting aroF. In the presence of Phe and ATP, TyrR strongly induced the expression of tyrP. This construct integrates ParoF, PtyrP and TyrR, and exhibits a Phe / Tyr-dependent dose-response relationship by the ratio of fluorescence expression intensity of GFP and RFP.