Team:NWU-China/Basic Part
Basic Parts
Number |
Part Number |
Part Name |
Long Description |
[1] |
arof promotor |
The aroF promoter is present in E. coli, which initiates 3-deoxy-D-arabinose-heptanoic acid heptaphosphate synthase, which is sensitive to phenylalanine, which is two of the three isozymes that control carbon. Flow through the oxalate pathway. The tyrR gene product complexed with phenylalanine inhibits the transcription of aroF. | |
[2] |
tyrP promotor |
The tyrP promoter ,which codes for a component of the tyrosine-specific transport system, has been localized on the Escherichia coli K-12 chromosome at min 42. The tyrR gene product complexed with phenylalanine promotes transcription of tyrP. | |
[3] |
TyrR |
The biosynthesis of Phe and Tyr in E. coli is controlled by the DNA binding protein TyrR. The TyrR protein binds to the consensus DNA sequence TGTAAAN6TTTACA (referred to as the TyrR box), which is classified as strong or weak. In the presence of Phe, binding of the TyrR dimer to the strong cassette in vitro strongly induces gene expression by promoting binding of the RNA polymerase to the promoter or promoting binding of the second dimer to the adjacent weak box. In the presence of Tyr and ATP, the TyrR dimer self-associates to form a hexamer, and the hexamer binds weakly to the weak consensus of the consensus sequence, due to weak-frame-bound RNA polymerase forming an open complex or leaving the initiation Impaired ability of the child leads to inhibition of gene expression. Thereby regulating the repression of aroF, tyrP. | |
[4] |
Kp-Sp |
The part is a signal peptide from actinomycetes, and E.coli can also be secreted normally. The signal peptide efficiently enabled extracellular protein secretion and also contributed to the active expression of the intracellular recombinant proteins. |